##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545670_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2406208
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84823755884778	31.0	31.0	34.0	31.0	34.0
2	32.50575885376493	33.0	31.0	34.0	31.0	34.0
3	32.4926589887491	34.0	31.0	34.0	31.0	34.0
4	35.98485874870335	37.0	35.0	37.0	35.0	37.0
5	35.760811201691624	37.0	35.0	37.0	35.0	37.0
6	35.76856157073703	37.0	35.0	37.0	35.0	37.0
7	35.75129290568396	37.0	35.0	37.0	33.0	37.0
8	35.713201435619865	37.0	35.0	37.0	33.0	37.0
9	37.872853469026786	39.0	38.0	39.0	35.0	39.0
10	37.79392346796287	39.0	38.0	39.0	35.0	39.0
11	37.62824452416416	39.0	37.0	39.0	35.0	39.0
12	37.15294687741043	39.0	37.0	39.0	33.0	39.0
13	36.91583395949145	39.0	37.0	39.0	33.0	39.0
14	37.62425692209485	40.0	36.0	41.0	33.0	41.0
15	37.52731102215602	40.0	36.0	41.0	32.0	41.0
16	37.46440041758651	40.0	36.0	41.0	32.0	41.0
17	37.5568076409022	40.0	36.0	41.0	32.0	41.0
18	37.654417240737295	40.0	36.0	41.0	33.0	41.0
19	37.62460934383063	40.0	35.0	41.0	33.0	41.0
20	37.58015516530574	40.0	35.0	41.0	32.0	41.0
21	37.553999487991064	40.0	35.0	41.0	32.0	41.0
22	37.46462068117137	39.0	35.0	41.0	32.0	41.0
23	37.39379679562199	39.0	35.0	41.0	32.0	41.0
24	37.39509053248929	39.0	35.0	41.0	32.0	41.0
25	37.31460663417294	39.0	35.0	41.0	32.0	41.0
26	37.2242977331968	39.0	35.0	41.0	32.0	41.0
27	37.13565577040721	39.0	35.0	41.0	32.0	41.0
28	37.04190369244886	39.0	35.0	41.0	31.0	41.0
29	36.98764071933931	39.0	35.0	41.0	31.0	41.0
30	36.89374775580499	39.0	35.0	41.0	31.0	41.0
31	36.75562835798069	39.0	35.0	41.0	31.0	41.0
32	36.65041343059286	39.0	35.0	41.0	31.0	41.0
33	36.551702928425144	39.0	35.0	41.0	30.0	41.0
34	36.47975652977631	39.0	35.0	41.0	30.0	41.0
35	36.37979966819161	38.0	35.0	41.0	30.0	41.0
36	36.248798940075005	38.0	35.0	41.0	30.0	41.0
37	36.1304031072958	38.0	35.0	41.0	30.0	41.0
38	36.02542506715962	38.0	35.0	40.0	30.0	41.0
39	35.952213191877014	38.0	35.0	40.0	29.0	41.0
40	35.87367634053249	38.0	35.0	40.0	29.0	41.0
41	35.79480576907732	38.0	35.0	40.0	29.0	41.0
42	35.64621553913876	38.0	34.0	40.0	29.0	41.0
43	35.5032303940474	37.0	34.0	40.0	28.0	41.0
44	35.43780753783547	37.0	34.0	40.0	28.0	41.0
45	35.415572136739634	37.0	34.0	40.0	28.0	41.0
46	35.34259008365029	37.0	34.0	40.0	28.0	41.0
47	35.23122481514483	37.0	34.0	40.0	28.0	41.0
48	35.16266715096949	36.0	34.0	40.0	28.0	41.0
49	35.1076689961965	36.0	34.0	40.0	28.0	41.0
50	34.98638480131393	36.0	34.0	40.0	28.0	41.0
51	34.806821355427296	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	12.0
11	34.0
12	23.0
13	35.0
14	59.0
15	89.0
16	191.0
17	369.0
18	786.0
19	1434.0
20	2611.0
21	4232.0
22	6547.0
23	9396.0
24	12777.0
25	18048.0
26	23813.0
27	29213.0
28	34433.0
29	41309.0
30	49880.0
31	61579.0
32	76499.0
33	100208.0
34	162790.0
35	260615.0
36	190275.0
37	262783.0
38	382579.0
39	673282.0
40	296.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.818792057876958	90.194613267016	3.4828244274809164	3.5037702476261403
2	3.786164787084076	3.0412167194191024	89.8612256297045	3.3113928637923236
3	28.661404167885735	16.22918716918903	41.78408516636966	13.325323496555578
4	28.043128441099025	16.0897977232226	41.44500392318536	14.422069912493019
5	24.78480663350799	14.139010426363804	45.29467111737638	15.781511822751815
6	23.17276810649786	14.839240830385403	46.379739407399526	15.608251655717211
7	23.787926895762958	17.240321701199562	43.73146461153816	15.240286791499322
8	26.319669787483047	19.056249501289997	42.705742811926484	11.918337899300475
9	81.38107761257547	3.9779187834135707	9.503085352554725	5.137918251456234
10	82.05770240976675	4.486644546107402	9.4147305636088	4.040922480517063
11	75.70708766656914	7.202910139106844	10.355921017634385	6.73408117668963
12	44.43830292310557	21.461153815463998	17.011455368779423	17.08908789265101
13	35.00777987605394	23.188311234938958	23.235356211931805	18.5685526770753
14	29.260230204537596	22.183119663802962	27.556221241056466	21.000428890602972
15	24.254968814001117	24.25455322233157	31.087337420538873	20.403140543128444
16	20.43701126419661	28.977461632577068	30.335365853658537	20.250161249567785
17	18.962658257307762	22.451591882331037	35.520329082107615	23.065420778253586
18	23.97988868792723	20.58920093358513	33.05994328004894	22.370967098438705
19	24.321920631965316	20.680880455887436	28.20811833390962	26.789080578237623
20	25.603065071681254	21.162592760060644	29.93926543341224	23.295076734845864
21	26.9460911109929	24.091599728701755	26.542593159028645	22.419716001276697
22	27.918076907731997	22.10145590073676	26.25342447535708	23.72704271617416
23	26.418622164002446	24.154977458307844	27.882502260818683	21.543898116871027
24	29.76425978136553	19.93684668989547	25.868170997685986	24.43072253105301
25	27.886034790009838	22.62796898688725	24.18710269436391	25.298893528738997
26	26.484909035295367	21.467263013006356	28.818123786472327	23.229704165225947
27	27.893889472564297	21.78706080272362	24.9956778466367	25.323371878075378
28	24.1381875548581	24.711330026331886	24.335344242891722	26.81513817591829
29	22.372546346782986	25.351424315769876	26.067654999069074	26.20837433837806
30	23.204560869218287	23.651238795648588	27.50439695986382	25.639803375269306
31	23.380688618772773	23.145629970476367	26.84551792696226	26.628163483788605
32	24.372082546479774	25.73514010426364	26.844104915285794	23.048672433970797
33	25.6190237917919	24.433797909407666	25.64645284198207	24.300725456818366
34	27.5309948267149	23.74915219299412	23.930017687581458	24.789835292709526
35	27.044295422507115	26.02829846796287	22.791379631353564	24.13602647817645
36	23.649742665638215	25.77819540122882	26.35661588690587	24.215446046227093
37	24.351552318004096	25.526970236986994	26.91475549910897	23.20672194589994
38	26.132653536186396	26.217392677607254	23.250899340372904	24.399054445833443
39	24.163871120036173	27.90893389100194	24.821877410431682	23.1053175785302
40	25.68107162805543	24.356456299704764	26.805413330850865	23.15705874138894
41	23.85662419873926	26.397136074686813	23.87042184216826	25.87581788440567
42	27.008388302258158	22.98841164188632	26.065826395723064	23.937373660132458
43	23.723343950315183	23.698117535973616	27.08365195361332	25.49488656009788
44	22.651699271218448	25.017496409287975	24.97917885735564	27.351625462137935
45	22.618368819320693	21.984840878261565	26.959764076921033	28.437026225496716
46	24.733356384817938	23.291959797324253	26.314142418278053	25.660541399579756
47	22.459114091549857	24.589561667154296	25.654307524536534	27.297016716759316
48	22.07190733303189	24.54546739101524	25.98690553767588	27.39571973827699
49	23.0862834800649	25.02323157432774	27.460842952895177	24.429641992712185
50	22.419674442109745	24.327198646168576	28.776107468681012	24.477019443040668
51	22.994853312764317	23.69633049179456	26.638387038859484	26.670429156581644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	912.0
1	1709.0
2	2506.0
3	6356.5
4	10207.0
5	6989.5
6	3772.0
7	3948.5
8	4125.0
9	4359.0
10	4593.0
11	4811.0
12	5029.0
13	4973.5
14	4918.0
15	4831.5
16	4745.0
17	4966.5
18	5188.0
19	5737.5
20	6287.0
21	6944.0
22	7601.0
23	7713.0
24	7825.0
25	10116.5
26	15815.5
27	19223.0
28	20210.0
29	21197.0
30	27528.0
31	33859.0
32	40481.5
33	47104.0
34	53899.5
35	60695.0
36	66682.0
37	72669.0
38	82730.5
39	92792.0
40	105901.0
41	119010.0
42	143734.5
43	168459.0
44	184636.5
45	200814.0
46	201456.5
47	202099.0
48	182739.0
49	163379.0
50	155384.0
51	147389.0
52	150877.5
53	154366.0
54	158130.0
55	161894.0
56	156190.5
57	150487.0
58	141356.0
59	132225.0
60	119266.0
61	106307.0
62	95556.0
63	84805.0
64	72572.5
65	60340.0
66	52508.5
67	44677.0
68	38950.5
69	33224.0
70	27779.0
71	22334.0
72	17677.0
73	13020.0
74	10062.0
75	5295.0
76	3486.0
77	2753.5
78	2021.0
79	1293.5
80	566.0
81	409.0
82	252.0
83	198.5
84	145.0
85	110.5
86	76.0
87	60.0
88	44.0
89	29.0
90	14.0
91	11.0
92	8.0
93	6.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2406208.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.542410181186604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7624744387091	12.036666979632313
2	10.4787220307304	3.466866360139579
3	4.39239733523203	2.179825151944777
4	2.3453103172763936	1.5518834108222
5	1.4063843569376187	1.163249345243322
6	0.9470406879116424	0.9399801310624512
7	0.6895222893648949	0.7984452377821453
8	0.4840633578100252	0.6406059694860748
9	0.3746551717735836	0.557792957518339
>10	3.0757104876594896	11.458165846256101
>50	1.3447665658557908	16.448845040996645
>100	1.684813955631955	45.00030425707121
>500	0.008331913723791821	0.9203698287059893
>1k	0.005049644681085952	1.91387305594457
>5k	7.574467021628929E-4	0.9231264273942537
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	9208	0.38267680931989256	Illumina Single End Adapter 2 (95% over 21bp)
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA	6529	0.27133980104795596	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6365	0.26452409766736706	No Hit
ATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTG	4203	0.17467317871106738	No Hit
ATGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCT	3857	0.16029370694470305	No Hit
ATTGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC	3610	0.15002859270686492	No Hit
ATGCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC	3562	0.14803375269303404	No Hit
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	3303	0.13726992845173816	Illumina Single End Adapter 2 (95% over 22bp)
ATGTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC	3031	0.12596583504002978	No Hit
ATATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC	2816	0.11703061414474558	No Hit
CCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	2630	0.10930060909115089	Illumina Single End Adapter 2 (95% over 21bp)
ATGGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTC	2475	0.10285893821315531	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1559166954810226E-5	0.0	0.0	0.14903117269994948	0.0
2	4.1559166954810226E-5	0.0	0.0	0.6983186823416762	0.0
3	8.311833390962045E-5	0.0	0.0	0.9623856291725403	0.0
4	8.311833390962045E-5	0.0	0.0	1.2754923930100805	0.0
5	8.311833390962045E-5	0.0	0.0	2.5680240444716333	0.0
6	1.2467750086443067E-4	0.0	0.0	2.8756865574380934	0.0
7	1.2467750086443067E-4	0.0	0.0	3.327351583902971	0.0
8	1.2467750086443067E-4	0.0	0.0	4.710731574327739	0.0
9	1.2467750086443067E-4	0.0	0.0	4.886402173045722	0.0
10	1.2467750086443067E-4	0.0	0.0	5.221659972870176	0.0
11	2.077958347740511E-4	0.0	0.0	5.471596802936404	0.0
12	2.077958347740511E-4	0.0	0.0	6.178227318669043	0.0
13	2.077958347740511E-4	0.0	0.0	6.826965914833631	0.0
14	2.4935500172886134E-4	0.0	0.0	7.097432973375535	0.0
15	2.9091416868367155E-4	0.0	0.0	7.2643345878660535	0.0
16	2.9091416868367155E-4	0.0	0.0	7.421137324786552	0.0
17	3.324733356384818E-4	0.0	0.0	7.581680386732984	0.0
18	3.324733356384818E-4	0.0	0.0	7.9030574247945315	0.0
19	3.324733356384818E-4	0.0	0.0	8.283947189935366	0.0
20	3.324733356384818E-4	0.0	0.0	8.705564938691916	0.0
21	3.324733356384818E-4	0.0	0.0	8.988333510652446	0.0
22	3.324733356384818E-4	0.0	0.0	9.29420897943985	0.0
23	3.324733356384818E-4	0.0	0.0	9.740429755033647	0.0
24	3.324733356384818E-4	0.0	0.0	10.131085924408863	0.0
25	3.74032502593292E-4	0.0	0.0	10.547799691464744	0.0
26	4.155916695481022E-4	0.0	0.0	10.870465063701891	0.0
27	4.571508365029125E-4	0.0	0.0	11.161545469053381	0.0
28	5.40269170412533E-4	0.0	0.0	11.437165864297683	0.0
29	5.40269170412533E-4	0.0	0.0	11.715570723727957	0.0
30	5.818283373673431E-4	0.0	0.0	11.98429229725776	0.0
31	5.818283373673431E-4	0.0	0.0	12.293741854403278	0.0
32	6.649466712769636E-4	0.0	0.0	12.645789557677475	0.0
33	6.649466712769636E-4	0.0	0.0	12.950916961459692	0.0
34	6.649466712769636E-4	0.0	0.0	13.27715642205495	0.0
35	6.649466712769636E-4	0.0	0.0	13.596372383434849	0.0
36	6.649466712769636E-4	0.0	0.0	13.929261310742879	0.0
37	7.065058382317738E-4	0.0	0.0	14.318088876772084	0.0
38	7.896241721413943E-4	0.0	0.0	14.647736189057637	0.0
39	8.311833390962044E-4	0.0	0.0	14.985820012235019	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAC	35	1.2126657E-7	45.000004	2
CCTTACG	20	7.034828E-4	45.0	37
CGTAGAT	25	3.892082E-5	45.0	31
ACGTATG	20	7.034828E-4	45.0	3
ACGTATC	20	7.034828E-4	45.0	12
ACGGGTA	165	0.0	40.90909	7
CGCGAAT	50	1.0822987E-9	40.5	4
CGGTAAG	50	1.0822987E-9	40.5	3
CGCCCGT	50	1.0822987E-9	40.5	25
TACACAA	2020	0.0	39.987625	32
ATACGTA	125	0.0	39.6	1
ATTACGT	245	0.0	39.489796	1
ATCGGTT	385	0.0	39.15584	1
ATTGTTT	4435	0.0	39.114994	1
TCGTTGC	185	0.0	38.91892	2
GGACTAA	2505	0.0	38.353294	10
GCATAGA	885	0.0	38.135593	40
ACTAATC	2460	0.0	37.957317	12
CACGTTA	30	1.1402929E-4	37.500004	12
CCGTTTA	30	1.1402929E-4	37.500004	42
>>END_MODULE