FastQCFastQC Report
Thu 26 May 2016
SRR1545669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545669_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1954008
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT70490.36074570830825664Illumina Single End Adapter 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC61320.31381652480440203Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC57700.2952905003459556Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT56970.2915545893363794Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC54020.2764574147086399Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC42220.21606871619768186Illumina Single End Adapter 2 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC41300.21136044478835295Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC38070.19483031799255685Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC31850.1629983091164417Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC25140.12865863394622745Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTACG206.312545E-446.035
GTCGACA206.312545E-446.012
CTCCGAT206.312545E-446.026
GGTCGAA206.312545E-446.032
CGACGTA206.312545E-446.042
CTATCGA405.6152203E-946.031
CCAATCG253.41804E-546.043
ACGATAA253.41804E-546.039
CCCGTAG253.41804E-546.026
TACGAAC206.312545E-446.037
CGAATTA206.312545E-446.035
TCCCGTA253.41804E-546.025
AATTACG206.312545E-446.031
CATCGAA206.312545E-446.036
CGACTAT253.41804E-546.023
TTCGACA206.312545E-446.035
CCGTTTA206.312545E-446.040
CGATAAG301.8620776E-646.026
TCGAATT206.312545E-446.034
CCGTTAG206.312545E-446.034
Produced by FastQC (version 0.11.5)