##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545669_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1954008
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51535408248073	33.0	31.0	34.0	31.0	34.0
2	32.93232985740079	34.0	31.0	34.0	31.0	34.0
3	33.01987555833958	34.0	33.0	34.0	31.0	34.0
4	36.41012933416854	37.0	37.0	37.0	35.0	37.0
5	36.38538685614388	37.0	37.0	37.0	35.0	37.0
6	36.39308641520403	37.0	37.0	37.0	35.0	37.0
7	36.458154726080956	37.0	37.0	37.0	35.0	37.0
8	36.493024081784725	37.0	37.0	37.0	35.0	37.0
9	38.312338025228144	39.0	39.0	39.0	37.0	39.0
10	38.05929453717692	39.0	39.0	39.0	35.0	39.0
11	37.88479576337456	39.0	38.0	39.0	35.0	39.0
12	37.49309419408723	39.0	37.0	39.0	35.0	39.0
13	37.40628185759731	39.0	37.0	39.0	35.0	39.0
14	38.61738795337583	41.0	38.0	41.0	35.0	41.0
15	38.69287996773811	41.0	38.0	41.0	35.0	41.0
16	38.79893429300187	41.0	38.0	41.0	35.0	41.0
17	38.75870057850326	40.0	38.0	41.0	35.0	41.0
18	38.74446573401951	40.0	38.0	41.0	35.0	41.0
19	38.70539526962018	40.0	38.0	41.0	35.0	41.0
20	38.589753470814856	40.0	38.0	41.0	34.0	41.0
21	38.55232322487932	40.0	37.0	41.0	35.0	41.0
22	38.69320596435634	40.0	38.0	41.0	35.0	41.0
23	38.70318801151275	40.0	38.0	41.0	35.0	41.0
24	38.65076447998166	40.0	37.0	41.0	35.0	41.0
25	38.57109489828087	40.0	37.0	41.0	35.0	41.0
26	38.45617674032041	40.0	37.0	41.0	35.0	41.0
27	38.384815722351185	40.0	36.0	41.0	35.0	41.0
28	38.30021422634913	40.0	36.0	41.0	34.0	41.0
29	38.20937990018464	40.0	36.0	41.0	34.0	41.0
30	38.159173350364995	40.0	36.0	41.0	34.0	41.0
31	38.10325239200658	40.0	36.0	41.0	34.0	41.0
32	38.03389494822949	40.0	35.0	41.0	34.0	41.0
33	37.94963633721049	40.0	35.0	41.0	34.0	41.0
34	37.81392655506016	40.0	35.0	41.0	34.0	41.0
35	37.738037920008516	40.0	35.0	41.0	33.0	41.0
36	37.63697845658769	40.0	35.0	41.0	33.0	41.0
37	37.532043881089535	40.0	35.0	41.0	33.0	41.0
38	37.43521930309395	40.0	35.0	41.0	33.0	41.0
39	37.33904057711125	39.0	35.0	41.0	33.0	41.0
40	37.175218320498175	39.0	35.0	41.0	33.0	41.0
41	37.00221493463691	39.0	35.0	41.0	33.0	41.0
42	36.96038245493365	39.0	35.0	41.0	33.0	41.0
43	36.88352299478815	39.0	35.0	41.0	33.0	41.0
44	36.73054818608726	38.0	35.0	41.0	32.0	41.0
45	36.641925212179274	38.0	35.0	40.0	32.0	41.0
46	36.62617195016602	38.0	35.0	41.0	32.0	41.0
47	36.57018036773647	38.0	35.0	40.0	32.0	41.0
48	36.46631334160352	38.0	35.0	40.0	32.0	41.0
49	36.39438835460244	37.0	35.0	40.0	32.0	41.0
50	36.27877828545226	37.0	35.0	40.0	32.0	41.0
51	36.1723693045269	37.0	35.0	40.0	32.0	41.0
52	35.8101062022264	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	4.0
11	6.0
12	14.0
13	25.0
14	39.0
15	55.0
16	94.0
17	226.0
18	396.0
19	677.0
20	1221.0
21	1931.0
22	2638.0
23	3645.0
24	4845.0
25	6326.0
26	8094.0
27	10110.0
28	12812.0
29	16119.0
30	20799.0
31	26949.0
32	35166.0
33	50040.0
34	122473.0
35	194803.0
36	129724.0
37	190625.0
38	312916.0
39	800289.0
40	940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.976395183643056	13.965091238111615	45.89924913306394	12.159264445181392
2	26.36539870870539	13.223538491142309	47.70640652443593	12.704656275716374
3	25.990169948127136	13.663659514188273	44.7737163819186	15.572454155765994
4	23.594171569410154	14.317648648316691	46.27621790698912	15.81196187528403
5	23.61592173624673	16.881148900106858	44.283237325538074	15.21969203810834
6	25.60639465140368	18.873822420379035	43.5963414684075	11.923441459809785
7	86.25102865494921	2.54717483244695	6.535848369095726	4.665948143508112
8	87.20604009809581	2.8915439445488453	6.726431007447258	3.1759849499080866
9	80.22244535334553	4.4984974472980666	8.508153497836242	6.770903701520157
10	46.332614810174775	20.533385738441194	14.98970321513525	18.144296236248778
11	35.783579186983886	22.006818805245423	23.466741180179408	18.74286082759129
12	29.741843431551967	22.361525643702585	26.60178463957159	21.294846285173858
13	24.992937592886005	23.03194255090051	31.79613389505058	20.1789859611629
14	20.31225051279217	29.117536878047584	30.199057526888325	20.371155082271926
15	19.1715182332928	21.73348317918862	35.305689638937	23.789308948581585
16	24.505529148294173	21.070026325378404	31.122697552927114	23.301746973400313
17	24.86888487662282	21.073711059524832	26.836891148859166	27.220512914993183
18	26.263249689868207	21.068695726936635	28.218871161223497	24.44918342197166
19	26.87194730011341	24.535723497549654	26.000814735661265	22.591514466675676
20	28.024654965588674	21.90528390876598	24.650717909036196	25.419343216609143
21	26.047078619944237	24.67446397353542	27.563295544337585	21.715161862182754
22	30.175260285525958	19.592652640112014	25.2179110832709	25.014175991091133
23	28.303620046591416	22.746682715730948	23.03291491130026	25.916782326377373
24	27.073072372272783	20.71700832340502	27.418260314185	24.791658990137194
25	30.01395081289329	21.752111557373357	23.159219409541823	25.07471822019152
26	24.34810911726052	24.248723649033167	23.334295458360458	28.068871775345855
27	22.55886362798924	23.93946186504866	24.81269268088974	28.688981826072357
28	23.70906362717041	22.587983263118677	25.6751763554704	28.027776754240513
29	23.620169415887755	22.16623473394172	25.18848438696259	29.02511146320793
30	24.023801335511422	27.08320539117547	25.27594564607719	23.61704762723592
31	25.00071647608403	23.807118496956	24.739509766592562	26.452655260367408
32	27.786119606470393	24.557217780070502	21.82176326811354	25.83489934534557
33	27.714318467478126	26.082237124924767	21.813626146873503	24.3898182607236
34	22.428464980696088	26.265501471846587	26.46672889773225	24.839304649725076
35	23.831785744991834	26.056392809036605	26.146054673266434	23.965766772705127
36	26.86821138910383	24.924565303724446	22.254258938550915	25.95296436862081
37	24.717094300535106	28.353005719526227	23.145504010218996	23.784395969719675
38	25.13904753716464	23.856043578122506	27.61355122394586	23.391357660767
39	24.020884254312165	26.58100683313477	23.23879943173211	26.15930948082096
40	28.40213550814531	22.261577229980634	26.098460190541694	23.237827071332358
41	24.282142140666775	23.215974550769495	26.124253329566717	26.377629978997014
42	22.578720250889454	24.788895439527373	23.772625291196352	28.85975901838682
43	22.350317910673855	20.89402909302316	27.201679829355868	29.553973166947117
44	26.886993297878004	21.986245706261183	24.64191548857528	26.484845507285538
45	22.324678302238272	23.35404972753438	25.30214820000737	29.019123770219977
46	22.225702248916075	23.4792795116499	25.029273165718873	29.265745073715156
47	23.32226889552141	24.091303617999518	27.72342794911792	24.86299953736116
48	22.074372264596665	23.143866350598362	29.891433402524452	24.89032798228052
49	23.285779792099113	22.34688906084315	25.627581872745658	28.739749274312082
50	21.716953052392824	23.16735653078186	28.784170791521834	26.33151962530348
51	24.009676521283435	23.247550675329887	26.454497627440627	26.28827517594606
52	22.19387024003996	22.39857769261948	26.94026841241182	28.467283654928742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	197.0
2	154.0
3	132.5
4	111.0
5	178.5
6	246.0
7	356.5
8	467.0
9	610.0
10	753.0
11	1008.5
12	1264.0
13	1160.5
14	1201.0
15	1345.0
16	1461.0
17	1577.0
18	1588.5
19	1600.0
20	1987.0
21	2374.0
22	2951.5
23	3529.0
24	4147.5
25	4766.0
26	5480.0
27	6194.0
28	8229.5
29	10265.0
30	12512.5
31	14760.0
32	20457.0
33	26154.0
34	29974.5
35	33795.0
36	37488.0
37	41181.0
38	43980.5
39	55666.5
40	64553.0
41	76663.5
42	88774.0
43	103989.5
44	119205.0
45	142042.0
46	164879.0
47	172709.0
48	180539.0
49	166275.5
50	152012.0
51	138204.5
52	124397.0
53	124428.5
54	124460.0
55	123138.5
56	121817.0
57	121670.0
58	121523.0
59	116024.0
60	110525.0
61	100973.5
62	91422.0
63	87727.5
64	73078.0
65	62123.0
66	52763.0
67	43403.0
68	39720.5
69	36038.0
70	30501.0
71	24964.0
72	20587.5
73	16211.0
74	14227.0
75	12243.0
76	9048.5
77	5854.0
78	4600.5
79	3347.0
80	2340.0
81	1333.0
82	1207.0
83	1081.0
84	666.0
85	251.0
86	205.0
87	159.0
88	150.0
89	99.5
90	58.0
91	38.0
92	18.0
93	16.0
94	14.0
95	12.0
96	10.0
97	8.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1954008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.620821035918834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.6178232841013	12.267232049993721
2	11.982418901937267	4.222801180968952
3	5.02348397415949	2.6555373625641208
4	2.579299656812183	1.8179751060277736
5	1.5288078152500824	1.3469424455417882
6	1.023737391643129	1.082345601955315
7	0.7272056172967297	0.896977202709039
8	0.5147649023576271	0.7256464176012782
9	0.41002577776686955	0.6502491765129091
>10	3.5354106823521176	14.157817924046388
>50	1.5905282366312306	20.644109085214694
>100	1.4563279285229456	35.97750021778928
>500	0.005228141744118816	0.624857637576294
>1k	0.0034854278294125444	1.3920470869389485
>5k	0.00145226159558856	1.5379615045595574
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCTT	7049	0.36074570830825664	Illumina Single End Adapter 2 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	6132	0.31381652480440203	Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	5770	0.2952905003459556	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGCT	5697	0.2915545893363794	Illumina Single End Adapter 2 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	5402	0.2764574147086399	Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	4222	0.21606871619768186	Illumina Single End Adapter 2 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	4130	0.21136044478835295	Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	3807	0.19483031799255685	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	3185	0.1629983091164417	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCATAGATCGTATGCCGTCTTCTGC	2514	0.12865863394622745	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3772246582409079	0.0
2	0.0	0.0	0.0	0.8475912074054968	0.0
3	0.0	0.0	0.0	3.1355040511604866	0.0
4	0.0	0.0	0.0	3.631203147581791	0.0
5	0.0	0.0	0.0	4.392356633135586	0.0
6	0.0	0.0	0.0	6.794649766019382	0.0
7	0.0	0.0	0.0	7.093983238553783	0.0
8	5.117686314487965E-5	0.0	0.0	7.672332968954068	0.0
9	1.023537262897593E-4	0.0	0.0	8.041727567133808	0.0
10	1.023537262897593E-4	0.0	0.0	9.282305906628837	0.0
11	1.023537262897593E-4	0.0	0.0	10.348934088294419	0.0
12	1.023537262897593E-4	0.0	0.0	10.7858821458254	0.0
13	1.023537262897593E-4	0.0	0.0	11.011111520526017	0.0
14	1.023537262897593E-4	0.0	0.0	11.155072036552562	0.0
15	1.023537262897593E-4	0.0	0.0	11.37958493516915	0.0
16	1.023537262897593E-4	0.0	0.0	11.877484636705685	0.0
17	1.023537262897593E-4	0.0	0.0	12.496519973306148	0.0
18	1.5353058943463896E-4	0.0	0.0	13.171031029555662	0.0
19	1.5353058943463896E-4	0.0	0.0	13.596106054837032	0.0
20	1.5353058943463896E-4	0.0	0.0	14.065807304780737	0.0
21	1.5353058943463896E-4	0.0	0.0	14.766265030644705	0.0
22	1.5353058943463896E-4	0.0	0.0	15.36672316592358	0.0
23	1.5353058943463896E-4	0.0	0.0	15.986526155471216	0.0
24	1.5353058943463896E-4	0.0	0.0	16.461549799181988	0.0
25	2.047074525795186E-4	0.0	0.0	16.895785482966293	0.0
26	2.047074525795186E-4	0.0	0.0	17.303972143409855	0.0
27	2.047074525795186E-4	0.0	0.0	17.715331769368397	0.0
28	2.047074525795186E-4	0.0	0.0	18.088308747968277	0.0
29	2.047074525795186E-4	0.0	0.0	18.506628427314524	0.0
30	2.047074525795186E-4	0.0	0.0	18.96194897871452	0.0
31	2.5588431572439824E-4	0.0	0.0	19.37310389722048	0.0
32	2.5588431572439824E-4	0.0	0.0	19.802426602142877	0.0
33	2.5588431572439824E-4	0.0	0.0	20.23297755178075	0.0
34	2.5588431572439824E-4	0.0	0.0	20.66265849474516	0.0
35	2.5588431572439824E-4	0.0	0.0	21.165215290827877	0.0
36	2.5588431572439824E-4	0.0	0.0	21.584916745479035	0.0
37	2.5588431572439824E-4	0.0	0.0	22.009224117813233	0.0
38	2.5588431572439824E-4	0.0	0.0	22.49392018865839	0.0
39	2.5588431572439824E-4	0.0	0.0	23.2229346041572	0.0
40	2.5588431572439824E-4	0.0	0.0	23.67927869282009	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	20	6.312545E-4	46.0	35
GTCGACA	20	6.312545E-4	46.0	12
CTCCGAT	20	6.312545E-4	46.0	26
GGTCGAA	20	6.312545E-4	46.0	32
CGACGTA	20	6.312545E-4	46.0	42
CTATCGA	40	5.6152203E-9	46.0	31
CCAATCG	25	3.41804E-5	46.0	43
ACGATAA	25	3.41804E-5	46.0	39
CCCGTAG	25	3.41804E-5	46.0	26
TACGAAC	20	6.312545E-4	46.0	37
CGAATTA	20	6.312545E-4	46.0	35
TCCCGTA	25	3.41804E-5	46.0	25
AATTACG	20	6.312545E-4	46.0	31
CATCGAA	20	6.312545E-4	46.0	36
CGACTAT	25	3.41804E-5	46.0	23
TTCGACA	20	6.312545E-4	46.0	35
CCGTTTA	20	6.312545E-4	46.0	40
CGATAAG	30	1.8620776E-6	46.0	26
TCGAATT	20	6.312545E-4	46.0	34
CCGTTAG	20	6.312545E-4	46.0	34
>>END_MODULE