FastQCFastQC Report
Thu 26 May 2016
SRR1545668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545668_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2065514
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC79710.385908785900265TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49580.24003710456574007No Hit
ATGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT38170.18479661721005036No Hit
ATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG36780.17806705740072448No Hit
ATGCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC32770.15865300356230944No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT29950.14500022754626693TruSeq Adapter, Index 13 (95% over 22bp)
ATTGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC28300.13701190115390163No Hit
ATGTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC25710.12447264942285552No Hit
ATATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC24210.11721053452070525No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC21440.10379982900140111TruSeq Adapter, Index 19 (95% over 21bp)
ATGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC21130.1022989919216234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC302.1663454E-645.00000420
TGGCACG302.1663454E-645.00000434
CGTATAT207.034595E-445.020
GATCGAC253.8918908E-545.042
CTATCGG253.8918908E-545.017
ATCGTAC351.2125565E-745.01
GTTACGT253.8918908E-545.024
CCCGTAG351.2125565E-745.041
CCGTAAA253.8918908E-545.027
CTACGAT453.8562575E-1045.040
CGTAGAC253.8918908E-545.030
TCTCGAC253.8918908E-545.026
AACGATC207.034595E-445.045
ACCGTAT351.2125565E-745.07
CGCACAT207.034595E-445.029
GCGTAGT207.034595E-445.011
ATCGAAT750.045.01
TACGTAC253.8918908E-545.027
TAATCGC207.034595E-445.039
ATTCGAT1400.043.3928571