##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545668_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2065514
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.870730481613776	31.0	31.0	34.0	31.0	34.0
2	32.536007986389826	34.0	31.0	34.0	31.0	34.0
3	32.5304515002077	34.0	31.0	34.0	31.0	34.0
4	36.0041190715725	37.0	35.0	37.0	35.0	37.0
5	35.78252822299921	37.0	35.0	37.0	35.0	37.0
6	35.7879394668833	37.0	35.0	37.0	35.0	37.0
7	35.77386403577996	37.0	35.0	37.0	35.0	37.0
8	35.74004533496263	37.0	35.0	37.0	33.0	37.0
9	37.887887470140605	39.0	38.0	39.0	35.0	39.0
10	37.810320336729745	39.0	38.0	39.0	35.0	39.0
11	37.648187327706324	39.0	37.0	39.0	35.0	39.0
12	37.223353605930534	39.0	37.0	39.0	33.0	39.0
13	37.01922427056897	39.0	37.0	39.0	33.0	39.0
14	37.75764724906246	40.0	36.0	41.0	33.0	41.0
15	37.68149429149354	40.0	36.0	41.0	33.0	41.0
16	37.62097037347605	40.0	36.0	41.0	32.0	41.0
17	37.728453547155816	40.0	36.0	41.0	33.0	41.0
18	37.76136642017435	40.0	36.0	41.0	33.0	41.0
19	37.73425791352661	40.0	36.0	41.0	33.0	41.0
20	37.68503675114282	40.0	36.0	41.0	33.0	41.0
21	37.65162085563206	40.0	36.0	41.0	33.0	41.0
22	37.603472065548814	40.0	35.0	41.0	33.0	41.0
23	37.5191259899473	40.0	35.0	41.0	32.0	41.0
24	37.49690198178274	40.0	35.0	41.0	32.0	41.0
25	37.44188710413001	39.0	35.0	41.0	32.0	41.0
26	37.32681840936445	39.0	35.0	41.0	32.0	41.0
27	37.258615531049415	39.0	35.0	41.0	32.0	41.0
28	37.177064401403236	39.0	35.0	41.0	32.0	41.0
29	37.09560961581476	39.0	35.0	41.0	31.0	41.0
30	37.01782849208478	39.0	35.0	41.0	31.0	41.0
31	36.924078461825964	39.0	35.0	41.0	31.0	41.0
32	36.78133481545029	39.0	35.0	41.0	31.0	41.0
33	36.69008827826875	39.0	35.0	41.0	31.0	41.0
34	36.622578689856375	39.0	35.0	41.0	31.0	41.0
35	36.53423215722576	39.0	35.0	41.0	30.0	41.0
36	36.41953140961523	39.0	35.0	41.0	30.0	41.0
37	36.272887523396115	38.0	35.0	41.0	30.0	41.0
38	36.19648765392053	38.0	35.0	41.0	30.0	41.0
39	36.14287242787994	38.0	35.0	41.0	30.0	41.0
40	36.073263604119845	38.0	35.0	40.0	30.0	41.0
41	35.9870414821686	38.0	35.0	40.0	30.0	41.0
42	35.8721688645054	38.0	35.0	40.0	29.0	41.0
43	35.759151475129194	38.0	35.0	40.0	29.0	41.0
44	35.70831909151911	38.0	35.0	40.0	29.0	41.0
45	35.675556302208555	38.0	35.0	40.0	29.0	41.0
46	35.603633284499644	37.0	34.0	40.0	29.0	41.0
47	35.51216065347415	37.0	34.0	40.0	29.0	41.0
48	35.448182389468194	37.0	34.0	40.0	29.0	41.0
49	35.39389033431872	37.0	34.0	40.0	29.0	41.0
50	35.292940643345915	37.0	34.0	40.0	28.0	41.0
51	35.1119043492322	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	16.0
11	28.0
12	39.0
13	39.0
14	54.0
15	86.0
16	139.0
17	317.0
18	610.0
19	1126.0
20	1953.0
21	3166.0
22	4821.0
23	7226.0
24	10023.0
25	13916.0
26	19153.0
27	23652.0
28	27937.0
29	33484.0
30	40614.0
31	50388.0
32	63172.0
33	82307.0
34	133568.0
35	220189.0
36	161052.0
37	223637.0
38	330760.0
39	611798.0
40	237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.413442852481271	90.75121253111817	3.087512357698859	3.7478322587017083
2	3.307844923830097	3.1415909066702037	89.99028813167087	3.5602760378288405
3	29.112559876137368	15.509844038820361	42.10554854626984	13.27204753877243
4	28.2045050287725	14.92224211503771	42.86579514832628	14.007457707863516
5	25.497721148343704	14.503024428786249	45.150650152940145	14.848604269929904
6	24.247911173683644	14.367900677506906	45.52910316754086	15.855084981268586
7	23.95776547629307	17.145369142983295	43.3543418248436	15.542523555880038
8	25.852935395257546	19.377355951109507	42.5776828431083	12.192025810524644
9	80.57156717407871	4.637586576513159	10.020072485589543	4.770773763818594
10	81.4114065554627	4.758186097988201	9.838761683532525	3.9916456630165666
11	74.6931272312848	7.610357518758043	10.953641563310633	6.7428736866465195
12	42.30651547266201	21.48796861217111	17.980754427227318	18.224761487939563
13	34.06992157884188	23.730848592650545	23.72847630178251	18.470753526725066
14	27.333777452004682	23.882675208204834	28.19530634989644	20.58824098989404
15	23.832469787181303	24.816583184621358	32.5453615903838	18.80558543781354
16	18.963318573488245	29.827732951701126	31.736265162085566	19.472683312725064
17	19.510543138414942	23.54450272426137	37.09599644446854	19.848957692855144
18	23.31700487142668	21.90704105612453	33.90255403739699	20.87340003505181
19	23.762462999524573	21.715175980409718	29.473293330376844	25.049067689688865
20	25.35572259495699	21.608035578553327	31.29405077864396	21.74219104784572
21	26.613569310108765	23.564110434497177	28.493682444176123	21.328637811217934
22	26.5921702781971	23.437555978802372	26.650944994805165	23.319328748195364
23	25.97125945406325	23.815137539614835	28.890532816528964	21.323070189792954
24	29.089272694351138	20.16263264252869	27.47388785551684	23.27420680760334
25	26.87708725285813	23.64292858823518	25.715003626216042	23.764980532690654
26	26.378954584669966	22.11313987704755	28.988135640813862	22.51976989746862
27	27.88695695115114	22.33787812621943	27.034917216731525	22.740247705897904
28	23.899668557075866	25.18690263053167	25.893651652808934	25.019777159583523
29	22.046183177649727	24.76487692651805	29.081235953859423	24.107703941972797
30	23.581733166659728	23.094687327222182	29.841288899518474	23.482290606599616
31	23.776309431938007	24.099860857878475	28.741804703332924	23.382025006850597
32	24.00259693228901	24.59005361377362	29.44952200759714	21.957827446340232
33	26.80553121402227	23.10451538938976	27.719105268712774	22.370848127875192
34	27.300081238858702	23.187932882565793	26.53136216941643	22.980623709159076
35	26.66750261678207	23.07004455065422	27.460525564096876	22.80192726846683
36	24.447474091194735	23.865197718340326	27.900367656670443	23.786960533794492
37	23.418722894156126	25.075889100727473	29.34523803760226	22.16014996751414
38	26.61826547774549	24.174660641370625	27.78678817960082	21.42028570128307
39	24.618182205494612	25.70212547578956	28.25185401793452	21.427838300781307
40	25.278889419292245	25.09065540102851	26.94515747654095	22.6852977031383
41	23.4286477845224	25.814639842673543	25.484019958228316	25.272692414575744
42	25.55659269315047	22.754094138311334	27.830264040815024	23.85904912772317
43	22.558888489741534	23.067188118792707	28.83766462004131	25.536258771424446
44	22.626377744232187	24.59625061849012	27.660621036700796	25.116750600576903
45	22.43465791081542	22.310040019094522	28.48002966816008	26.77527240192998
46	24.71094361984475	24.006421646137476	27.5058411610863	23.776793572931485
47	22.328001649952505	24.44214854026649	28.10457832771891	25.125271482062097
48	22.388858172832524	24.153358437657648	27.72273632616385	25.735047063345977
49	23.157528828175455	23.88809758733177	29.961113795403953	22.993259789088818
50	23.223468831486983	22.998488511818366	30.97979485977824	22.79824779691641
51	23.33588637017227	22.68655646972134	28.647155139108232	25.33040202099816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	672.0
1	1374.5
2	2077.0
3	5298.5
4	8520.0
5	5955.5
6	3391.0
7	3415.5
8	3440.0
9	3789.0
10	4138.0
11	4271.5
12	4405.0
13	4334.5
14	4264.0
15	4915.5
16	5567.0
17	5847.5
18	6128.0
19	6685.0
20	7242.0
21	7975.5
22	8709.0
23	10249.5
24	11790.0
25	13466.0
26	19311.0
27	23480.0
28	25276.0
29	27072.0
30	29981.5
31	32891.0
32	36463.5
33	40036.0
34	47059.0
35	54082.0
36	62841.5
37	71601.0
38	77739.0
39	83877.0
40	104330.5
41	124784.0
42	140107.0
43	155430.0
44	170833.0
45	186236.0
46	187601.5
47	188967.0
48	165771.5
49	142576.0
50	137101.0
51	131626.0
52	124247.0
53	116868.0
54	118860.0
55	120852.0
56	117437.0
57	114022.0
58	106598.0
59	99174.0
60	92205.5
61	85237.0
62	72553.0
63	59869.0
64	51770.5
65	43672.0
66	37139.0
67	30606.0
68	24912.0
69	19218.0
70	15827.5
71	12437.0
72	9697.0
73	6957.0
74	6090.0
75	3322.0
76	1421.0
77	1206.0
78	991.0
79	800.0
80	609.0
81	350.5
82	92.0
83	64.5
84	37.0
85	47.5
86	58.0
87	34.0
88	10.0
89	10.5
90	11.0
91	8.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2065514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.546961224948735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.75245618302134	12.03832071480236
2	10.958680068161936	3.6266570832898823
3	4.466417081886168	2.217168908052573
4	2.343704837266809	1.5512477225991472
5	1.3855005856427265	1.1462912233886982
6	0.9188507536867103	0.9122512675661351
7	0.6478516836778135	0.7503983682534174
8	0.48969441606076186	0.6482363611705072
9	0.34782623153422176	0.5179920449595128
>10	2.804692505672659	10.313415814753075
>50	1.094137283320147	13.419289749415352
>100	1.7781520100867156	49.681657475261126
>500	0.006458534624503818	0.7248155315393965
>1k	0.005284255601866759	2.065051475187406
>5k	2.935697556592644E-4	0.38720625976144557
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	7971	0.385908785900265	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4958	0.24003710456574007	No Hit
ATGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	3817	0.18479661721005036	No Hit
ATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	3678	0.17806705740072448	No Hit
ATGCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	3277	0.15865300356230944	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	2995	0.14500022754626693	TruSeq Adapter, Index 13 (95% over 22bp)
ATTGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	2830	0.13701190115390163	No Hit
ATGTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	2571	0.12447264942285552	No Hit
ATATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	2421	0.11721053452070525	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2144	0.10379982900140111	TruSeq Adapter, Index 19 (95% over 21bp)
ATGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	2113	0.1022989919216234	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8414099347668426E-5	0.0	0.0	0.15419890642232392	0.0
2	9.682819869533685E-5	0.0	0.0	0.6900945721016657	0.0
3	9.682819869533685E-5	0.0	0.0	0.9523053341686379	0.0
4	1.4524229804300528E-4	0.0	0.0	1.292511210284704	0.0
5	1.4524229804300528E-4	0.0	0.0	2.554618366179072	0.0
6	1.4524229804300528E-4	0.0	0.0	2.8575453857974336	0.0
7	1.4524229804300528E-4	0.0	0.0	3.308377478922922	0.0
8	1.4524229804300528E-4	0.0	0.0	4.694085830451887	0.0
9	1.4524229804300528E-4	0.0	0.0	4.8870644304516935	0.0
10	1.4524229804300528E-4	0.0	0.0	5.214585812538671	0.0
11	1.4524229804300528E-4	0.0	0.0	5.468905076411973	0.0
12	2.4207049673834213E-4	0.0	0.0	6.169699164469473	0.0
13	2.4207049673834213E-4	0.0	0.0	6.783638358297257	0.0
14	2.4207049673834213E-4	0.0	0.0	7.051465155888558	0.0
15	2.4207049673834213E-4	0.0	0.0	7.212538864418251	0.0
16	2.4207049673834213E-4	0.0	0.0	7.323261909626369	0.0
17	2.4207049673834213E-4	0.0	0.0	7.4875793628123555	0.0
18	2.9048459608601055E-4	0.0	0.0	7.797623255034824	0.0
19	3.38898695433679E-4	0.0	0.0	8.210062967377612	0.0
20	3.38898695433679E-4	0.0	0.0	8.651066998335523	0.0
21	3.38898695433679E-4	0.0	0.0	8.950798687396938	0.0
22	3.38898695433679E-4	0.0	0.0	9.256872623472898	0.0
23	3.38898695433679E-4	0.0	0.0	9.719227272243131	0.0
24	4.3572689412901583E-4	0.0	0.0	10.137767161103726	0.0
25	5.325550928243527E-4	0.0	0.0	10.534811189853954	0.0
26	6.293832915196895E-4	0.0	0.0	10.860105523370938	0.0
27	6.77797390867358E-4	0.0	0.0	11.160515009823222	0.0
28	7.746255895626948E-4	0.0	0.0	11.439477050264486	0.0
29	7.746255895626948E-4	0.0	0.0	11.738579356034382	0.0
30	8.230396889103632E-4	0.0	0.0	12.018073951568471	0.0
31	8.230396889103632E-4	0.0	0.0	12.344191324774366	0.0
32	8.230396889103632E-4	0.0	0.0	12.673552442636554	0.0
33	8.714537882580317E-4	0.0	0.0	12.987517876906184	0.0
34	8.714537882580317E-4	0.0	0.0	13.307438245395577	0.0
35	8.714537882580317E-4	0.0	0.0	13.652630773744454	0.0
36	8.714537882580317E-4	0.0	0.0	13.988769865515314	0.0
37	9.198678876057001E-4	0.0	0.0	14.366254598129085	0.0
38	9.682819869533685E-4	0.0	0.0	14.677992983828723	0.0
39	0.001016696086301037	0.0	0.0	15.02439586466129	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAC	30	2.1663454E-6	45.000004	20
TGGCACG	30	2.1663454E-6	45.000004	34
CGTATAT	20	7.034595E-4	45.0	20
GATCGAC	25	3.8918908E-5	45.0	42
CTATCGG	25	3.8918908E-5	45.0	17
ATCGTAC	35	1.2125565E-7	45.0	1
GTTACGT	25	3.8918908E-5	45.0	24
CCCGTAG	35	1.2125565E-7	45.0	41
CCGTAAA	25	3.8918908E-5	45.0	27
CTACGAT	45	3.8562575E-10	45.0	40
CGTAGAC	25	3.8918908E-5	45.0	30
TCTCGAC	25	3.8918908E-5	45.0	26
AACGATC	20	7.034595E-4	45.0	45
ACCGTAT	35	1.2125565E-7	45.0	7
CGCACAT	20	7.034595E-4	45.0	29
GCGTAGT	20	7.034595E-4	45.0	11
ATCGAAT	75	0.0	45.0	1
TACGTAC	25	3.8918908E-5	45.0	27
TAATCGC	20	7.034595E-4	45.0	39
ATTCGAT	140	0.0	43.392857	1
>>END_MODULE