FastQCFastQC Report
Thu 26 May 2016
SRR1545667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545667_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1665938
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT60260.36171814317219486TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT55800.3349464385829485TruSeq Adapter, Index 19 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC51960.31189636108906815TruSeq Adapter, Index 19 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC50040.30037132234212793TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC48850.293228199368764TruSeq Adapter, Index 19 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC37400.2244981505914386TruSeq Adapter, Index 13 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC35840.2151340566095497TruSeq Adapter, Index 13 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC33370.20030757447155895TruSeq Adapter, Index 19 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC25070.1504857923884322TruSeq Adapter, Index 13 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC18700.1122490752957193TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC17430.10462574237456616TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCATA351.01994374E-746.00000437
ATACACG351.01994374E-746.00000418
CTTAACG206.3122757E-446.01
GTCGATC301.8619121E-646.044
CAACGTA206.3122757E-446.011
CAACGAA206.3122757E-446.029
CCAATCG206.3122757E-446.013
TAAGTCG253.4178218E-546.041
TGACGTA206.3122757E-446.019
CGTTCAC206.3122757E-446.041
ATCGTAG253.4178218E-546.01
CGTTAGC206.3122757E-446.020
CCCGTTT253.4178218E-546.023
AATGCGC206.3122757E-446.037
GTATGCG750.046.01
CGTAGAT206.3122757E-446.022
TACGAAC253.4178218E-546.033
TCTAACG253.4178218E-546.035
ACTACGT301.8619121E-646.044
CGGTGAT253.4178218E-546.038
Produced by FastQC (version 0.11.5)