##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545667_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1665938
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55866304748436	33.0	31.0	34.0	31.0	34.0
2	32.9632963531656	34.0	31.0	34.0	31.0	34.0
3	33.04337676432136	34.0	33.0	34.0	31.0	34.0
4	36.418843918561194	37.0	37.0	37.0	35.0	37.0
5	36.398583260601534	37.0	37.0	37.0	35.0	37.0
6	36.395408472584215	37.0	37.0	37.0	35.0	37.0
7	36.46940822527609	37.0	37.0	37.0	35.0	37.0
8	36.50030613384172	37.0	37.0	37.0	35.0	37.0
9	38.29963239928497	39.0	39.0	39.0	37.0	39.0
10	38.0970108131275	39.0	39.0	39.0	35.0	39.0
11	37.94160526982397	39.0	38.0	39.0	35.0	39.0
12	37.55998962746513	39.0	37.0	39.0	35.0	39.0
13	37.48628880546575	39.0	37.0	39.0	35.0	39.0
14	38.72276459267992	41.0	38.0	41.0	35.0	41.0
15	38.81442646725148	41.0	38.0	41.0	35.0	41.0
16	38.86361677325327	41.0	38.0	41.0	35.0	41.0
17	38.82357926885634	41.0	38.0	41.0	35.0	41.0
18	38.807291747952206	40.0	38.0	41.0	35.0	41.0
19	38.76739890680205	40.0	38.0	41.0	35.0	41.0
20	38.69269564653666	40.0	38.0	41.0	35.0	41.0
21	38.64825521718095	40.0	38.0	41.0	35.0	41.0
22	38.76260761204799	40.0	38.0	41.0	35.0	41.0
23	38.78353035947316	40.0	38.0	41.0	35.0	41.0
24	38.724432721986055	40.0	38.0	41.0	35.0	41.0
25	38.64492976329251	40.0	37.0	41.0	35.0	41.0
26	38.539276971892114	40.0	37.0	41.0	35.0	41.0
27	38.45645396167204	40.0	37.0	41.0	35.0	41.0
28	38.386875141812	40.0	37.0	41.0	35.0	41.0
29	38.330264391592	40.0	36.0	41.0	35.0	41.0
30	38.227436435209476	40.0	36.0	41.0	34.0	41.0
31	38.19007730179634	40.0	36.0	41.0	34.0	41.0
32	38.13249352616964	40.0	36.0	41.0	34.0	41.0
33	38.058354512592906	40.0	36.0	41.0	34.0	41.0
34	37.93880624609079	40.0	35.0	41.0	34.0	41.0
35	37.831997349241085	40.0	35.0	41.0	34.0	41.0
36	37.78639060997468	40.0	35.0	41.0	34.0	41.0
37	37.70547583403464	40.0	35.0	41.0	33.0	41.0
38	37.61189552072166	40.0	35.0	41.0	33.0	41.0
39	37.50243286364799	40.0	35.0	41.0	33.0	41.0
40	37.373999512586906	39.0	35.0	41.0	33.0	41.0
41	37.250445694857795	39.0	35.0	41.0	33.0	41.0
42	37.21357517506654	39.0	35.0	41.0	33.0	41.0
43	37.11330673770573	39.0	35.0	41.0	33.0	41.0
44	36.988251063364906	39.0	35.0	41.0	33.0	41.0
45	36.91360062619377	39.0	35.0	41.0	33.0	41.0
46	36.90530499934572	39.0	35.0	41.0	33.0	41.0
47	36.860816548995224	38.0	35.0	41.0	33.0	41.0
48	36.76894578309637	38.0	35.0	41.0	33.0	41.0
49	36.69124541249434	38.0	35.0	40.0	33.0	41.0
50	36.57958099281006	38.0	35.0	40.0	32.0	41.0
51	36.498186607184664	38.0	35.0	40.0	32.0	41.0
52	36.16946248900019	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	5.0
12	6.0
13	27.0
14	29.0
15	55.0
16	102.0
17	148.0
18	297.0
19	572.0
20	937.0
21	1372.0
22	1959.0
23	2731.0
24	3544.0
25	4768.0
26	6064.0
27	7733.0
28	9957.0
29	12562.0
30	16531.0
31	21405.0
32	28210.0
33	40799.0
34	96443.0
35	163482.0
36	106907.0
37	156728.0
38	261223.0
39	720425.0
40	906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.87292324204142	13.319223164367461	45.84528355797154	11.962570035619573
2	26.3389754000449	12.45832678046842	48.87282720005186	12.329870619434818
3	26.88701500295929	14.094221993855713	43.97810722848029	15.0406557747047
4	24.511176286272356	13.975910267969155	45.670126979515445	15.84278646624304
5	24.72649042161233	16.621206791609293	42.971407099183764	15.680895687594617
6	25.6447118680287	18.571519468311546	43.51026268684669	12.273505976813063
7	85.2347446303524	2.9471685020691045	7.06106709853548	4.757019769043025
8	86.2544704544827	3.0523344806349337	7.373143538354969	3.3200515265273975
9	78.69524556135943	5.27246512175123	9.111083365647461	6.921205951241884
10	44.08795525403706	20.666615444272235	15.802328778141803	19.443100523548896
11	35.23588512897839	22.06174539508673	23.698000765934868	19.004368710000012
12	28.174758004199436	23.60718105955924	27.138825094331242	21.079235841910084
13	24.505053609438047	23.47110156560448	33.432036486351834	18.591808338605638
14	18.979037635254134	30.194400992113753	31.586829761971934	19.23973161066018
15	19.360624465016105	23.052898727323587	36.69104132326653	20.895435484393776
16	23.43124413993798	22.365598239550334	32.707939911329234	21.495217709182455
17	24.204982418313286	21.8114959860451	27.873846445666047	26.10967514997557
18	25.565237121669593	21.625114500059425	29.68369771263997	23.125950665631017
19	26.410826813482856	24.161043208090575	27.75061256781465	21.67751741061192
20	26.753336558743484	22.842866901409295	25.34818222526889	25.055614314578335
21	26.17276273186637	24.2819360624465	28.313058469162716	21.23224273652441
22	29.32798219381514	19.428574172628274	26.923570985234747	24.319872648321848
23	27.330128732281757	23.49859358511541	23.878499680060123	25.29277800254271
24	27.12826047547988	20.787208167410792	27.56399097685508	24.520540380254246
25	29.774997628963384	22.060664922704206	24.16488488767289	23.99945256065952
26	23.44961217044092	25.408208468742533	24.62858761850681	26.51359174230974
27	22.48306959802826	24.408051199984634	27.149689844399973	25.959189357587135
28	23.761568557773458	22.46992385070753	29.511662498844494	24.256845092674517
29	25.278251651622092	23.19306000583455	27.20401359474362	24.324674747799737
30	24.48932673364795	24.74395805846316	28.316840122501556	22.44987508538733
31	27.537879560944045	22.550719174423058	26.52613722719573	23.385264037437167
32	28.594641577297597	22.564825341639363	24.504693451977204	24.335839629085836
33	26.898720120436654	21.97068558373721	28.17775931637312	22.952834979453016
34	24.65289824711364	23.4371267117984	27.753493827501387	24.15648121358658
35	22.963339572060907	23.940866947029242	29.496715964219554	23.599077516690297
36	27.07015507179739	22.45233616136975	28.191745431102476	22.285763335730383
37	25.528621113150667	24.245740237631892	28.623454174164948	21.602184475052493
38	25.378915661927394	23.3560312568655	28.23028227941256	23.034770801794544
39	24.128809115345227	24.675528140903204	25.102074627026937	26.093588116724632
40	26.443961299880307	21.26765822017386	28.514626594747224	23.77375388519861
41	22.64814176758079	21.916481885880508	29.348030959135336	26.08734538740337
42	22.638297463651107	24.103898224303666	27.389014477129404	25.868789834915823
43	22.83470333229688	20.741648248614293	29.33230408334524	27.09134433574359
44	27.2049740146392	22.28528312578259	25.93241765299789	24.577325206580316
45	22.640518434659633	22.600000720314924	27.8037958195323	26.955685025493146
46	22.86711750377265	23.012080881761506	27.132282233792616	26.98851938067323
47	23.858450914739922	22.426704955406503	30.40593347411488	23.30891065573869
48	22.850130076869608	21.468265925862788	32.698635843590814	22.98296815367679
49	23.769431995668505	20.857558924761907	28.090301079632013	27.28270799993757
50	21.626855261120163	22.27970068513954	30.69513991517091	25.398304138569383
51	24.251862914466205	23.408734298635363	27.937114106287268	24.402288680611164
52	22.233960687612626	21.45632070341153	29.197245035529534	27.112473573446312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	230.0
1	174.5
2	119.0
3	104.5
4	90.0
5	146.0
6	202.0
7	256.5
8	311.0
9	498.5
10	686.0
11	882.0
12	1078.0
13	977.5
14	1183.0
15	1489.0
16	1475.0
17	1461.0
18	1678.5
19	1896.0
20	2674.0
21	3452.0
22	3872.0
23	4292.0
24	5075.0
25	5858.0
26	7520.0
27	9182.0
28	11277.0
29	13372.0
30	15420.5
31	17469.0
32	19689.0
33	21909.0
34	25119.5
35	28330.0
36	30868.0
37	33406.0
38	40288.5
39	56304.0
40	65437.0
41	75807.0
42	86177.0
43	98487.0
44	110797.0
45	132682.0
46	154567.0
47	162296.5
48	170026.0
49	153021.5
50	136017.0
51	124041.0
52	112065.0
53	106292.0
54	100519.0
55	97774.5
56	95030.0
57	93005.0
58	90980.0
59	85409.0
60	79838.0
61	76575.5
62	73313.0
63	67913.0
64	53371.5
65	44230.0
66	38559.0
67	32888.0
68	27016.5
69	21145.0
70	18016.5
71	14888.0
72	12022.0
73	9156.0
74	7785.5
75	6415.0
76	5082.5
77	3750.0
78	2814.5
79	1879.0
80	1170.0
81	461.0
82	461.0
83	461.0
84	382.0
85	303.0
86	207.5
87	112.0
88	70.0
89	25.0
90	22.0
91	14.5
92	7.0
93	8.0
94	9.0
95	9.5
96	10.0
97	6.5
98	3.0
99	7.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1665938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.152260647043487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.92894047370308	11.822871531295005
2	12.25229060492112	4.203089639578583
3	5.157501668048227	2.653884386937743
4	2.7767713316561027	1.9051162255121405
5	1.6619987992379515	1.4253518299801324
6	1.0692691846111304	1.1004230253781062
7	0.7400779956503656	0.8885807476375611
8	0.5646056441052469	0.7747410536388055
9	0.43417579628201847	0.6702386782020153
>10	3.4078495195854734	12.966881794257784
>50	1.3634133971226932	17.32730212767715
>100	1.632240339100208	40.66301952845758
>500	0.00560786889104477	0.6554585736421432
>1k	0.0038554098625932795	1.6321237105210076
>5k	0.0014019672227611926	1.3109171472842864
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT	6026	0.36171814317219486	TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	5580	0.3349464385829485	TruSeq Adapter, Index 19 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	5196	0.31189636108906815	TruSeq Adapter, Index 19 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	5004	0.30037132234212793	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	4885	0.293228199368764	TruSeq Adapter, Index 19 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	3740	0.2244981505914386	TruSeq Adapter, Index 13 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	3584	0.2151340566095497	TruSeq Adapter, Index 13 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	3337	0.20030757447155895	TruSeq Adapter, Index 19 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2507	0.1504857923884322	TruSeq Adapter, Index 13 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1870	0.1122490752957193	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1743	0.10462574237456616	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3737834181103979	0.0
2	0.0	0.0	0.0	0.9126990320167977	0.0
3	0.0	0.0	0.0	3.182951586433589	0.0
4	0.0	0.0	0.0	3.693594839663901	0.0
5	0.0	0.0	0.0	4.4822196264206715	0.0
6	0.0	0.0	0.0	6.953980280178494	0.0
7	6.002624347364668E-5	0.0	0.0	7.285325144153024	0.0
8	6.002624347364668E-5	0.0	0.0	7.864158209969399	0.0
9	6.002624347364668E-5	0.0	0.0	8.232719344897589	0.0
10	6.002624347364668E-5	0.0	0.0	9.477963765758389	0.0
11	6.002624347364668E-5	0.0	0.0	10.50033074460154	0.0
12	6.002624347364668E-5	0.0	0.0	10.95298864663631	0.0
13	6.002624347364668E-5	0.0	0.0	11.181868713001323	0.0
14	6.002624347364668E-5	0.0	0.0	11.329293166972601	0.0
15	6.002624347364668E-5	0.0	0.0	11.57059866573666	0.0
16	6.002624347364668E-5	0.0	0.0	12.062093547298879	0.0
17	6.002624347364668E-5	0.0	0.0	12.731986424464775	0.0
18	6.002624347364668E-5	0.0	0.0	13.459804626582741	0.0
19	6.002624347364668E-5	0.0	0.0	13.913482974756564	0.0
20	6.002624347364668E-5	0.0	0.0	14.412361084266042	0.0
21	6.002624347364668E-5	0.0	0.0	15.140719522575269	0.0
22	6.002624347364668E-5	0.0	0.0	15.76919429174435	0.0
23	6.002624347364668E-5	0.0	0.0	16.390045727992277	0.0
24	1.2005248694729336E-4	0.0	0.0	16.883941659293445	0.0
25	1.2005248694729336E-4	0.0	0.0	17.33239772428506	0.0
26	1.2005248694729336E-4	0.0	0.0	17.739975917471117	0.0
27	1.2005248694729336E-4	0.0	0.0	18.172465001698743	0.0
28	1.2005248694729336E-4	0.0	0.0	18.559154062155976	0.0
29	1.8007873042094003E-4	0.0	0.0	18.986841046905706	0.0
30	1.8007873042094003E-4	0.0	0.0	19.430555038662902	0.0
31	1.8007873042094003E-4	0.0	0.0	19.85734162976053	0.0
32	1.8007873042094003E-4	0.0	0.0	20.297213941935414	0.0
33	1.8007873042094003E-4	0.0	0.0	20.73450512564093	0.0
34	1.8007873042094003E-4	0.0	0.0	21.18770326386696	0.0
35	1.8007873042094003E-4	0.0	0.0	21.686221215915598	0.0
36	1.8007873042094003E-4	0.0	0.0	22.102923398109652	0.0
37	1.8007873042094003E-4	0.0	0.0	22.538293742024013	0.0
38	1.8007873042094003E-4	0.0	0.0	23.001756367884038	0.0
39	1.8007873042094003E-4	0.0	0.0	23.687916357031295	0.0
40	1.8007873042094003E-4	0.0	0.0	24.120165336285023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCATA	35	1.01994374E-7	46.000004	37
ATACACG	35	1.01994374E-7	46.000004	18
CTTAACG	20	6.3122757E-4	46.0	1
GTCGATC	30	1.8619121E-6	46.0	44
CAACGTA	20	6.3122757E-4	46.0	11
CAACGAA	20	6.3122757E-4	46.0	29
CCAATCG	20	6.3122757E-4	46.0	13
TAAGTCG	25	3.4178218E-5	46.0	41
TGACGTA	20	6.3122757E-4	46.0	19
CGTTCAC	20	6.3122757E-4	46.0	41
ATCGTAG	25	3.4178218E-5	46.0	1
CGTTAGC	20	6.3122757E-4	46.0	20
CCCGTTT	25	3.4178218E-5	46.0	23
AATGCGC	20	6.3122757E-4	46.0	37
GTATGCG	75	0.0	46.0	1
CGTAGAT	20	6.3122757E-4	46.0	22
TACGAAC	25	3.4178218E-5	46.0	33
TCTAACG	25	3.4178218E-5	46.0	35
ACTACGT	30	1.8619121E-6	46.0	44
CGGTGAT	25	3.4178218E-5	46.0	38
>>END_MODULE