SRR1545666_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545666_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1800649
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	4625	0.2568518350883487	TruSeq Adapter, Index 3 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4263	0.23674797253656876	No Hit
ATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG	2189	0.12156727935316657	RNA PCR Primer, Index 34 (95% over 24bp)
ATGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	2164	0.12017889105539169	RNA PCR Primer, Index 34 (95% over 23bp)
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA	2093	0.11623586828971111	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	1894	0.10518429743942323	TruSeq Adapter, Index 3 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	1893	0.10512876190751222	RNA PCR Primer, Index 34 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCACG	30	2.166189E-6	45.000004	1
ATCGACG	30	2.166189E-6	45.000004	1
GGTACGA	20	7.0343545E-4	45.0	10
TCGGACA	35	1.2124474E-7	45.0	38
ACTACCG	40	6.8193913E-9	45.0	43
AATCGAC	20	7.0343545E-4	45.0	19
TCGAAAT	70	0.0	41.785713	21
ATGCGTT	555	0.0	40.94595	1
CCGATGT	45	1.929402E-8	40.0	44
TCGACGT	90	0.0	40.0	28
ATTCGAC	40	3.4596815E-7	39.375	1
TAACACG	40	3.4596815E-7	39.375	29
CAAGTCG	110	0.0	38.863636	20
ATGCGAA	145	0.0	38.793106	1
ATCGGTT	215	0.0	38.720932	1
CGCGATC	35	6.2502622E-6	38.571426	42
CGATTAC	35	6.2502622E-6	38.571426	40
CCGTATG	35	6.2502622E-6	38.571426	31
TTAACGG	35	6.2502622E-6	38.571426	4
GCGTAAG	70	0.0	38.571426	3