FastQCFastQC Report
Thu 26 May 2016
SRR1545665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545665_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1490439
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT41130.2759589624265066RNA PCR Primer, Index 34 (96% over 27bp)
TGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC33130.22228350170654418TruSeq Adapter, Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT33000.22141127546984482TruSeq Adapter, Index 18 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC32620.21886169108564657RNA PCR Primer, Index 34 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC29840.20020946848545965TruSeq Adapter, Index 18 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC24930.16726615446858276RNA PCR Primer, Index 34 (96% over 25bp)
TTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC24200.16236826867788617TruSeq Adapter, Index 3 (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC21050.141233556019401RNA PCR Primer, Index 34 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC16690.11198042992702151RNA PCR Primer, Index 34 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC15270.10245303564922817TruSeq Adapter, Index 3 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA551.8189894E-1246.00000417
TTACGTC206.312059E-446.00000417
CGTAAGT206.312059E-446.00000420
TAATACG301.8617775E-646.01
TTATACG501.6370905E-1146.01
CCCGTAA253.417648E-546.017
CGTAAGC253.417648E-546.022
TCGAATT301.8617775E-646.041
GCGTAAG600.046.01
GGTTACG508.731149E-1041.3999981
AATCGTT2500.040.4822
GAATCGT355.374184E-639.42857442
TCGAAAT700.039.42857419
AATCGTC355.374184E-639.42857443
TCGTTTG2650.039.0566021
AACACGT3550.038.8732441
TTGGGAC21750.038.1747135
TAGGGCG10800.037.6944475
CGTAGTG1100.037.6363641
TTTGGGC30050.037.504164
Produced by FastQC (version 0.11.5)