##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545665_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1490439
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.490218653698676	33.0	31.0	34.0	31.0	34.0
2	32.916817796635755	34.0	31.0	34.0	31.0	34.0
3	33.014656084549586	34.0	33.0	34.0	31.0	34.0
4	36.39882880144709	37.0	37.0	37.0	35.0	37.0
5	36.37647565583026	37.0	37.0	37.0	35.0	37.0
6	36.39043597222027	37.0	37.0	37.0	35.0	37.0
7	36.451276436003084	37.0	37.0	37.0	35.0	37.0
8	36.47469571045846	37.0	37.0	37.0	35.0	37.0
9	38.27121472264212	39.0	39.0	39.0	37.0	39.0
10	38.06203071712428	39.0	39.0	39.0	35.0	39.0
11	37.89073487744215	39.0	38.0	39.0	35.0	39.0
12	37.6174489529595	39.0	37.0	39.0	35.0	39.0
13	37.574393853086235	39.0	37.0	39.0	35.0	39.0
14	38.86189639428383	41.0	38.0	41.0	35.0	41.0
15	38.92980725812999	41.0	38.0	41.0	35.0	41.0
16	38.98292784877476	41.0	38.0	41.0	35.0	41.0
17	38.93920717318857	41.0	38.0	41.0	35.0	41.0
18	38.915282678459164	41.0	38.0	41.0	35.0	41.0
19	38.90310908396788	40.0	38.0	41.0	35.0	41.0
20	38.77523132446212	40.0	38.0	41.0	35.0	41.0
21	38.733293345115094	40.0	38.0	41.0	35.0	41.0
22	38.85833167274877	40.0	38.0	41.0	35.0	41.0
23	38.860604828510255	40.0	38.0	41.0	35.0	41.0
24	38.800968707877345	41.0	38.0	41.0	35.0	41.0
25	38.71657209721431	40.0	38.0	41.0	35.0	41.0
26	38.57978421122904	40.0	38.0	41.0	35.0	41.0
27	38.488546663097246	40.0	38.0	41.0	35.0	41.0
28	38.38002159095407	40.0	37.0	41.0	34.0	41.0
29	38.34486953172857	40.0	37.0	41.0	34.0	41.0
30	38.25914982095879	40.0	37.0	41.0	34.0	41.0
31	38.16898779487118	40.0	37.0	41.0	34.0	41.0
32	38.135926394840716	40.0	36.0	41.0	34.0	41.0
33	38.06393082843377	40.0	36.0	41.0	34.0	41.0
34	37.937341950928555	40.0	36.0	41.0	34.0	41.0
35	37.853030550059415	40.0	36.0	41.0	33.0	41.0
36	37.76200099433791	40.0	35.0	41.0	33.0	41.0
37	37.67387595198462	40.0	35.0	41.0	33.0	41.0
38	37.57356993476419	40.0	35.0	41.0	33.0	41.0
39	37.46184110855929	40.0	35.0	41.0	33.0	41.0
40	37.335271017465324	40.0	35.0	41.0	33.0	41.0
41	37.178475603496686	39.0	35.0	41.0	33.0	41.0
42	37.08965613487033	39.0	35.0	41.0	33.0	41.0
43	37.001580071374946	39.0	35.0	41.0	32.0	41.0
44	36.84702225317507	39.0	35.0	41.0	32.0	41.0
45	36.74530255850793	39.0	35.0	41.0	32.0	41.0
46	36.67996811677633	39.0	35.0	41.0	32.0	41.0
47	36.65141411355983	38.0	35.0	41.0	32.0	41.0
48	36.53498667171216	38.0	35.0	40.0	32.0	41.0
49	36.44658184601986	38.0	35.0	40.0	31.0	41.0
50	36.32496599995035	38.0	35.0	40.0	31.0	41.0
51	36.203847322835756	37.0	35.0	40.0	31.0	41.0
52	35.846763269077094	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	4.0
12	8.0
13	24.0
14	37.0
15	44.0
16	93.0
17	174.0
18	332.0
19	573.0
20	913.0
21	1481.0
22	2170.0
23	2920.0
24	3806.0
25	4762.0
26	6073.0
27	7838.0
28	9661.0
29	12608.0
30	15919.0
31	20129.0
32	26135.0
33	37348.0
34	81186.0
35	125217.0
36	96344.0
37	145646.0
38	252025.0
39	636235.0
40	727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.19119802957384	13.836326075740102	45.301686281692845	11.670789612993218
2	26.692940804689087	13.256295628335007	47.296534779350246	12.75422878762566
3	27.272166120183382	14.349262197245242	43.671629633953486	14.706942048617892
4	24.784778176094427	14.472380285271655	45.12610043081266	15.616741107821252
5	23.859144855978677	17.16158796166767	42.68232379855868	16.296943383794975
6	26.097210285023404	19.94734437303372	42.58698276145484	11.368462580488032
7	86.48277453824006	3.042861868214667	6.355845492502545	4.118518101042714
8	86.4494286582678	3.2315311126453348	6.935070807996839	3.383969421090028
9	79.11581755442523	5.6495435237537395	8.665366378630726	6.569272543190295
10	43.855937747200656	22.65419785714142	15.04892182772995	18.440942567927973
11	33.690409335772884	23.531321979631503	24.067137266268528	18.711131418327085
12	27.070279293550424	23.930264841432624	27.49196713183163	21.507488733185323
13	24.090955751963012	23.46489859699055	32.29753113008986	20.146614520956575
14	19.78470772705223	29.096393747077204	31.032400520920344	20.086498004950222
15	19.961568370124507	22.85152226961318	35.154742998539355	22.032166361722954
16	24.32122347845165	22.01304447884147	31.344590419332828	22.321141623374054
17	24.23648334484001	22.145757055471577	27.210909000636725	26.40685059905169
18	25.84647878913528	21.906029029031043	28.495094398361825	23.75239778347185
19	27.42869718250797	23.915705372712335	26.542045665740094	22.113551779039597
20	27.513168938816012	22.34086735518864	26.06124772634103	24.084715979654316
21	27.50431248779722	23.345135225259135	27.737800741929057	21.41275154501459
22	30.536439263867894	19.80416508156322	25.455989812397554	24.203405842171332
23	28.984681694453784	23.032140194935856	23.459128484963156	24.52404962564721
24	27.25760665146309	20.71134746205648	28.139762848395673	23.89128303808475
25	29.744659123922553	22.42990152565788	23.46717980407115	24.358259546348425
26	25.188417640708543	24.326121364242347	25.284563809723178	25.200897185325932
27	25.409023784267585	22.369516632347917	26.14766521810017	26.07379436528432
28	24.63676809315913	22.60998269637335	28.67202213576	24.08122707470752
29	26.127000165722986	21.44046150161127	26.673483450178104	25.759054882487643
30	27.633066499199227	22.974237791684196	26.57290905565407	22.819786653462504
31	25.71993889048797	24.1051797490538	25.00498175369807	25.169899606760154
32	28.197799440299132	24.45615016783646	22.913919992700137	24.432130399164272
33	27.922645609783427	24.56410493820948	23.548229749758292	23.9650197022488
34	23.936169142111822	23.318230400573253	25.202641637799335	27.542958819515594
35	24.97834530631579	25.5375765126919	26.13713140893388	23.346946772058434
36	26.32982631291854	25.994623060722375	23.675910251945904	23.999640374413175
37	27.28712815485907	25.128166936050384	23.60754113385385	23.977163775236694
38	25.60238963151125	23.855924328335476	26.753661169628547	23.788024870524723
39	24.956673839050104	26.085602966642714	23.177533599161052	25.78018959514613
40	26.326605785275348	24.02701485938036	26.30339114851396	23.342988206830338
41	24.657433145536313	23.429741170218975	26.320097635663046	25.59272804858166
42	24.118732802885592	23.43369973544707	25.188082169079046	27.25948529258829
43	24.215415726507423	21.638255574364333	27.43185061582527	26.71447808330297
44	27.097251212562206	22.26840548321669	25.20613054274613	25.428212761474978
45	23.212758120258528	23.316754325403455	26.42738146277707	27.043106091560944
46	23.54863231571369	24.05043077911944	25.427273440912373	26.973663464254493
47	24.130205932614484	23.36539771168092	28.37338529117931	24.131011064525286
48	23.976694114955393	22.961020209481905	29.17053297719665	23.89175269836605
49	23.512736851357218	22.69505830161449	26.37504788857511	27.41715695845318
50	23.286025124141275	23.819223732068203	27.90667716021924	24.988073983571283
51	24.599866213914158	23.69422700291659	26.47763511287614	25.22827167029312
52	23.533133526430802	22.972627527862596	26.46951669944225	27.024722246264353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	82.0
2	68.0
3	83.5
4	99.0
5	119.0
6	139.0
7	251.0
8	363.0
9	481.0
10	599.0
11	746.0
12	893.0
13	934.5
14	967.5
15	959.0
16	1025.5
17	1092.0
18	1292.0
19	1492.0
20	2242.0
21	2992.0
22	3579.5
23	4167.0
24	4283.5
25	4400.0
26	6991.0
27	9582.0
28	10888.0
29	12194.0
30	14321.0
31	16448.0
32	20292.5
33	24137.0
34	27394.5
35	30652.0
36	35053.5
37	39455.0
38	41302.5
39	47604.0
40	52058.0
41	58209.0
42	64360.0
43	67920.5
44	71481.0
45	77068.5
46	82656.0
47	90170.5
48	97685.0
49	112687.5
50	127690.0
51	131990.0
52	136290.0
53	126718.5
54	117147.0
55	108365.0
56	99583.0
57	95102.5
58	90622.0
59	87726.5
60	84831.0
61	78860.5
62	72890.0
63	65477.5
64	50464.5
65	42864.0
66	37817.5
67	32771.0
68	27840.0
69	22909.0
70	19305.5
71	15702.0
72	13470.0
73	11238.0
74	8950.5
75	6663.0
76	5519.0
77	4375.0
78	3303.5
79	2232.0
80	1589.5
81	947.0
82	848.5
83	750.0
84	496.5
85	243.0
86	180.5
87	118.0
88	111.0
89	63.0
90	22.0
91	29.0
92	36.0
93	35.0
94	34.0
95	63.0
96	92.0
97	53.5
98	15.0
99	14.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1490439.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.806707291409722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38947091453267	14.337970613841664
2	10.712021904847301	4.243397647369594
3	4.06609523437916	2.416078743790321
4	1.9785792400274183	1.5675655944033189
5	1.1730204455791664	1.161683630621278
6	0.7698827960572241	0.9149305914118515
7	0.534569065603695	0.7411637106618352
8	0.4074610524373332	0.6456369438620807
9	0.33351355471374433	0.5945224820339426
>10	4.719203080003196	24.10533520154346
>50	2.3640142727950635	33.66503744104673
>100	0.5464025523757495	13.422507053349722
>500	0.002035018817038864	0.28369425680279226
>1k	0.0037308678312379176	1.9004760892614239
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCTT	4113	0.2759589624265066	RNA PCR Primer, Index 34 (96% over 27bp)
TGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	3313	0.22228350170654418	TruSeq Adapter, Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT	3300	0.22141127546984482	TruSeq Adapter, Index 18 (95% over 24bp)
GTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	3262	0.21886169108564657	RNA PCR Primer, Index 34 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2984	0.20020946848545965	TruSeq Adapter, Index 18 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2493	0.16726615446858276	RNA PCR Primer, Index 34 (96% over 25bp)
TTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2420	0.16236826867788617	TruSeq Adapter, Index 3 (95% over 24bp)
ATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	2105	0.141233556019401	RNA PCR Primer, Index 34 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1669	0.11198042992702151	RNA PCR Primer, Index 34 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1527	0.10245303564922817	TruSeq Adapter, Index 3 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.292464166597895	0.0
2	0.0	0.0	0.0	0.651955564769843	0.0
3	0.0	0.0	0.0	2.347898840542954	0.0
4	0.0	0.0	0.0	2.7464391363886747	0.0
5	0.0	0.0	0.0	3.3344538085758626	0.0
6	0.0	0.0	0.0	5.261268659770712	0.0
7	0.0	0.0	0.0	5.511262118073937	0.0
8	0.0	0.0	0.0	5.96307530868422	0.0
9	0.0	0.0	0.0	6.252050570335317	0.0
10	0.0	0.0	0.0	7.164533402574678	0.0
11	0.0	0.0	0.0	8.000729986265792	0.0
12	0.0	0.0	0.0	8.34458840650305	0.0
13	0.0	0.0	0.0	8.534264065822217	0.0
14	0.0	0.0	0.0	8.667580491385424	0.0
15	0.0	0.0	0.0	8.87483486409038	0.0
16	0.0	0.0	0.0	9.295985947764384	0.0
17	0.0	0.0	0.0	9.8450859109296	0.0
18	0.0	0.0	0.0	10.402304287528708	0.0
19	0.0	0.0	0.0	10.775080362228847	0.0
20	6.709432589995297E-5	0.0	0.0	11.18187326016026	0.0
21	6.709432589995297E-5	0.0	0.0	11.772974271338848	0.0
22	4.696602812996708E-4	0.0	0.0	12.315767367869467	0.0
23	4.696602812996708E-4	0.0	0.0	12.830112470218506	0.0
24	4.696602812996708E-4	0.0	0.0	13.272264077899196	0.0
25	4.696602812996708E-4	0.0	0.0	13.640679021415838	0.0
26	4.696602812996708E-4	0.0	0.0	13.987690874970395	0.0
27	4.696602812996708E-4	0.0	0.0	14.34953057454884	0.0
28	4.696602812996708E-4	0.0	0.0	14.686411184892505	0.0
29	4.696602812996708E-4	0.0	0.0	15.061803938302742	0.0
30	4.696602812996708E-4	0.0	0.0	15.467053666738458	0.0
31	4.696602812996708E-4	0.0	0.0	15.832650648567302	0.0
32	4.696602812996708E-4	0.0	0.0	16.208043401977537	0.0
33	4.696602812996708E-4	0.0	0.0	16.580081439092776	0.0
34	4.696602812996708E-4	0.0	0.0	16.964666115151307	0.0
35	4.696602812996708E-4	0.0	0.0	17.38883644349081	0.0
36	4.696602812996708E-4	0.0	0.0	17.742893201264863	0.0
37	4.696602812996708E-4	0.0	0.0	18.111710710736904	0.0
38	4.696602812996708E-4	0.0	0.0	18.509311686020023	0.0
39	4.696602812996708E-4	0.0	0.0	19.03110425854396	0.0
40	4.696602812996708E-4	0.0	0.0	19.420050065785986	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAA	55	1.8189894E-12	46.000004	17
TTACGTC	20	6.312059E-4	46.000004	17
CGTAAGT	20	6.312059E-4	46.000004	20
TAATACG	30	1.8617775E-6	46.0	1
TTATACG	50	1.6370905E-11	46.0	1
CCCGTAA	25	3.417648E-5	46.0	17
CGTAAGC	25	3.417648E-5	46.0	22
TCGAATT	30	1.8617775E-6	46.0	41
GCGTAAG	60	0.0	46.0	1
GGTTACG	50	8.731149E-10	41.399998	1
AATCGTT	250	0.0	40.48	22
GAATCGT	35	5.374184E-6	39.428574	42
TCGAAAT	70	0.0	39.428574	19
AATCGTC	35	5.374184E-6	39.428574	43
TCGTTTG	265	0.0	39.056602	1
AACACGT	355	0.0	38.87324	41
TTGGGAC	2175	0.0	38.174713	5
TAGGGCG	1080	0.0	37.694447	5
CGTAGTG	110	0.0	37.636364	1
TTTGGGC	3005	0.0	37.50416	4
>>END_MODULE