FastQCFastQC Report
Thu 26 May 2016
SRR1545664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545664_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1569551
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC94340.6010636162826184TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50800.3236594414580985No Hit
ATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTG43040.2742185504007197No Hit
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT36440.23216830800655727TruSeq Adapter, Index 15 (95% over 22bp)
ATGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCT31820.2027331383306436No Hit
ATTGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC30010.19120117791648694No Hit
ATATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC27630.17603760565919807No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27250.17361653109710995No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC25580.16297654552161733TruSeq Adapter, Index 14 (95% over 21bp)
ATGTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC25460.16221199565990527No Hit
ATGCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC24940.1588989462591531No Hit
ATGGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC23070.1469847109141404No Hit
ATTTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC20580.13112030128361551No Hit
ATAGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTC19080.12156342801221495No Hit
ATAGCATTCCCACGAATAAATAATATAAGTTTTTGACTCCTACCACCATCA17670.11257996713709845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGA406.8175723E-945.00000418
CTTAACG406.8175723E-945.0000043
CTAGACG207.0340745E-445.00000428
TTAGGCG351.2123019E-745.0000046
AGATCGC351.2123019E-745.00000422
CGAACCG207.0340745E-445.0000043
GATCGCC351.2123019E-745.00000423
CGCGGTC207.0340745E-445.00000443
CGAGTAG750.045.0000043
CGTGCAT207.0340745E-445.00000443
GCAACGC351.2123019E-745.00000411
GACGTGC207.0340745E-445.00000441
GACGTAC351.2123019E-745.00000429
CGGCACG207.0340745E-445.00000433
CCGGATA750.045.00000422
CGCGCAC207.0340745E-445.00000427
GCCGGAC207.0340745E-445.00000425
CGCGATG351.2123019E-745.0000043
CACGACA207.0340745E-445.00000425
ACGGGTC406.8175723E-945.0000047