FastQCFastQC Report
Thu 26 May 2016
SRR1545663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545663_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1296939
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT76360.5887709445085698TruSeq Adapter, Index 15 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC55810.43032093259590465TruSeq Adapter, Index 15 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC53300.4109676708002458TruSeq Adapter, Index 15 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC51230.3950070126659774TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT46550.3589220464493704TruSeq Adapter, Index 14 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC40330.31096296741789703TruSeq Adapter, Index 15 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC38410.2961588787136481TruSeq Adapter, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC37210.28690632327349247TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC26770.20640909094413848TruSeq Adapter, Index 15 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC21950.16924465992618004TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC20750.1599921044860244TruSeq Adapter, Index 15 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC20180.1555971406519505TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT18450.14225803989239277TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT13080.10085285429769633TruSeq Adapter, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGT551.8189894E-1246.00000443
CCGGGTA351.0197073E-746.0000045
AAATCGA351.0197073E-746.00000446
CTCAAGT351.0197073E-746.00000417
GGTAACC351.0197073E-746.0000048
CACGGGC1550.046.0000044
ACCGAAA351.0197073E-746.00000424
CGCGACA351.0197073E-746.00000438
ACGGGTC351.0197073E-746.0000045
GCCGCGA351.0197073E-746.00000436
CTTATCG700.046.0000041
CGGATGA351.0197073E-746.0000046
TGCGACA551.8189894E-1246.00000436
CATCGCG351.0197073E-746.0000041
CAATGAG700.046.00000417
CCGGTCA351.0197073E-746.00000434
TAGGCCA351.0197073E-746.00000419
CGACTTG351.0197073E-746.0000041
CGGTAAG551.8189894E-1246.00000422
ATGCCCG551.8189894E-1246.00000443
Produced by FastQC (version 0.11.5)