##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545663_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1296939
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.593483579412755	33.0	31.0	34.0	31.0	34.0
2	32.98356360630685	34.0	33.0	34.0	31.0	34.0
3	33.0447160583497	34.0	33.0	34.0	31.0	34.0
4	36.40414082697799	37.0	37.0	37.0	35.0	37.0
5	36.376718565792224	37.0	37.0	37.0	35.0	37.0
6	36.39503939661002	37.0	37.0	37.0	35.0	37.0
7	36.499926365079624	37.0	37.0	37.0	35.0	37.0
8	36.563938627799764	37.0	37.0	37.0	35.0	37.0
9	38.38734820990039	39.0	39.0	39.0	37.0	39.0
10	38.108749910365866	39.0	39.0	39.0	35.0	39.0
11	37.933630648781474	39.0	38.0	39.0	35.0	39.0
12	37.641074098319194	39.0	37.0	39.0	35.0	39.0
13	37.56770750204905	39.0	37.0	39.0	35.0	39.0
14	38.959813838584544	41.0	38.0	41.0	35.0	41.0
15	39.004091942643406	41.0	38.0	41.0	35.0	41.0
16	39.065974575519746	41.0	38.0	41.0	35.0	41.0
17	39.038922416551586	41.0	38.0	41.0	35.0	41.0
18	39.04765759993338	41.0	38.0	41.0	35.0	41.0
19	39.010244892011116	41.0	38.0	41.0	35.0	41.0
20	38.95993489285155	41.0	38.0	41.0	35.0	41.0
21	38.93239928786165	41.0	38.0	41.0	35.0	41.0
22	38.99996838710224	41.0	38.0	41.0	35.0	41.0
23	39.01360819591361	41.0	38.0	41.0	35.0	41.0
24	38.97924651814773	41.0	38.0	41.0	35.0	41.0
25	38.90327841170633	41.0	38.0	41.0	35.0	41.0
26	38.8136859173793	41.0	38.0	41.0	35.0	41.0
27	38.75981214228271	41.0	38.0	41.0	35.0	41.0
28	38.74597417457567	41.0	38.0	41.0	35.0	41.0
29	38.74602120839916	40.0	38.0	41.0	35.0	41.0
30	38.72606190422217	40.0	38.0	41.0	35.0	41.0
31	38.68270211629074	40.0	38.0	41.0	35.0	41.0
32	38.64828106796079	40.0	38.0	41.0	35.0	41.0
33	38.553560344781054	40.0	37.0	41.0	35.0	41.0
34	38.518198619981355	40.0	37.0	41.0	35.0	41.0
35	38.43213828869361	40.0	37.0	41.0	35.0	41.0
36	38.29720827270982	40.0	37.0	41.0	35.0	41.0
37	38.29233294703914	40.0	37.0	41.0	34.0	41.0
38	38.228854248349386	40.0	37.0	41.0	34.0	41.0
39	38.21158666675919	40.0	36.0	41.0	34.0	41.0
40	38.087554618991334	40.0	36.0	41.0	34.0	41.0
41	38.01217790505182	40.0	36.0	41.0	34.0	41.0
42	37.95108328148047	40.0	36.0	41.0	34.0	41.0
43	37.91940484479224	40.0	36.0	41.0	34.0	41.0
44	37.8299634755374	40.0	35.0	41.0	34.0	41.0
45	37.75914210305959	40.0	35.0	41.0	34.0	41.0
46	37.7655895921088	40.0	35.0	41.0	34.0	41.0
47	37.77560162814134	40.0	35.0	41.0	34.0	41.0
48	37.72952852832709	40.0	35.0	41.0	34.0	41.0
49	37.71649321980448	40.0	35.0	41.0	34.0	41.0
50	37.637428591475775	40.0	35.0	41.0	34.0	41.0
51	37.514219250095806	40.0	35.0	41.0	34.0	41.0
52	37.16582661173733	39.0	35.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	8.0
14	14.0
15	12.0
16	21.0
17	46.0
18	81.0
19	182.0
20	336.0
21	556.0
22	895.0
23	1399.0
24	2028.0
25	2891.0
26	3726.0
27	4720.0
28	6273.0
29	7888.0
30	10541.0
31	13553.0
32	18373.0
33	24857.0
34	52997.0
35	136818.0
36	60585.0
37	82619.0
38	161967.0
39	702296.0
40	1249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.989502975853146	13.800571962135457	47.53893590986161	12.670989152149792
2	24.682039787530485	13.855624667004385	50.07159164771821	11.390743897746926
3	23.963810171488404	13.008630321086805	46.867971431192984	16.159588076231802
4	21.062825622484944	14.685578889986346	49.51821172776823	14.733383759760482
5	21.834642955451258	16.81289559493546	46.71322244145638	14.639239008156899
6	22.266043352848513	19.04384092081432	45.91449559308495	12.775620133252218
7	84.7126194832602	2.3650302751324466	7.426023891640239	5.496326349967115
8	87.62378184324783	2.0747313481975636	7.053454325916639	3.248032482637965
9	79.3573174991268	4.718649065222034	8.344108705189681	7.579924730461494
10	36.34079937452725	31.960331210642906	13.947533384376598	17.751336030453242
11	28.17765523282128	22.881646708133534	31.742741948541912	17.19795611050327
12	28.473274379134256	25.347606942192346	27.852736327614487	18.326382351058918
13	19.681110676755036	32.705855865233445	32.81781178605933	14.795221671952188
14	14.05085358679167	39.35597587858797	27.88673946885705	18.70643106576331
15	12.594424255882505	26.51204104433593	39.090735955970175	21.802798743811387
16	15.434958776010282	25.24875880824002	36.75616200916157	22.560120406588126
17	15.550615719012228	24.559751846463097	29.78682883312168	30.102803601402993
18	18.282818235861516	24.70578801316022	33.64182895263386	23.36956479834441
19	19.096966009966547	30.98819605239722	29.467769879693652	20.447068057942587
20	24.5698525528186	23.087361857419666	28.298786604458652	24.043998985303087
21	18.489535745320325	29.625680159205636	29.990847680577115	21.893936414896924
22	24.935020074190074	23.212117146604427	25.59364781227182	26.25921496693368
23	20.821256820868214	28.84684630503054	23.788936873669464	26.542960000431787
24	19.521735409298355	22.541692400336487	36.25205194693042	21.684520243434733
25	23.198623836587533	28.458932918202013	26.577579978703703	21.76486326650675
26	18.372336709745024	31.778749810129852	26.412653177982925	23.4362603021422
27	16.543800440884265	31.8628709600066	25.330181296113384	26.263147302995748
28	18.017809627129726	25.845008901729376	32.54409035428806	23.593091116852836
29	18.232237599455335	21.803415580840735	31.412502823957023	28.551843995746907
30	16.153188392052364	32.54825400423613	29.104838392553546	22.193719211157966
31	19.012150918431782	29.53662431309414	30.84108042089875	20.61014434757533
32	26.835417856969375	25.47652588132518	24.47547648732901	23.212579774376437
33	20.826422830988967	27.88697078274306	27.868003044090738	23.418603342177235
34	19.523971443529728	25.31059672043172	29.904721810354996	25.26071002568355
35	19.438462410336953	25.959663484558643	32.933468729061275	21.66840537604313
36	21.377566716707573	26.07825040344997	30.24891687272879	22.295266007113675
37	24.740716409946806	27.8517339674418	27.00520224929623	20.402347373315166
38	16.609647793766708	25.56342279783398	36.60742718046107	21.219502227938243
39	19.83323810911693	30.477146573585962	27.20490323754625	22.48471207975086
40	24.173149238321926	25.67992789175127	30.96421651288149	19.18270635704532
41	20.008882453222547	25.693421201768164	31.068539075469236	23.22915726954005
42	18.949233541438726	28.586772392533494	25.63528431175252	26.82870975427526
43	19.70894544770417	23.334867715443824	30.098023114425583	26.85816372242642
44	24.20082980001372	24.752590522761672	26.420363640849725	24.626216036374878
45	16.975277942910193	29.22797448453628	27.715490088585508	26.081257483968017
46	18.446125839380265	28.301639475719366	28.27712020380295	24.975114481097414
47	19.9384859272487	26.697400571653716	31.657772647749816	21.706340853347765
48	18.5078866469433	22.188553201037212	36.29507632972715	23.008483822292337
49	18.864881077675975	22.539070842961774	30.068183623131077	28.52786445623117
50	18.089516931790932	24.373081540457957	34.76532049695476	22.77208103079636
51	20.046895035155856	23.615374354537877	31.436482363472763	24.901248246833504
52	19.294199650099195	21.567552521745434	32.353796130735525	26.784451697419847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	526.0
1	413.5
2	301.0
3	229.0
4	157.0
5	172.5
6	188.0
7	382.5
8	577.0
9	642.5
10	708.0
11	933.0
12	1158.0
13	1204.5
14	1288.0
15	1325.0
16	1281.0
17	1237.0
18	2039.0
19	2841.0
20	2526.5
21	2212.0
22	2528.5
23	2845.0
24	5239.5
25	7634.0
26	9693.5
27	11753.0
28	13628.0
29	15503.0
30	19707.5
31	23912.0
32	30268.5
33	36625.0
34	44027.0
35	51429.0
36	56093.0
37	60757.0
38	63052.5
39	73073.0
40	80798.0
41	89254.5
42	97711.0
43	104654.5
44	111598.0
45	120438.5
46	129279.0
47	142781.0
48	156283.0
49	154443.5
50	152604.0
51	125521.0
52	98438.0
53	78766.5
54	59095.0
55	49719.5
56	40344.0
57	32953.5
58	25563.0
59	22336.5
60	19110.0
61	16454.0
62	13798.0
63	11327.5
64	8033.5
65	7210.0
66	5086.5
67	2963.0
68	2473.0
69	1983.0
70	1447.0
71	911.0
72	827.5
73	744.0
74	673.0
75	602.0
76	509.0
77	416.0
78	280.5
79	145.0
80	118.5
81	92.0
82	73.5
83	55.0
84	44.0
85	33.0
86	22.0
87	11.0
88	7.0
89	2.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1296939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.04162706602235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.33104501001552	10.319746328181669
2	12.986890936265368	4.166617222933497
3	6.1044779966490195	2.9377727836494834
4	3.6325370560962975	2.33087219029614
5	2.3715364726466928	1.902165183383418
6	1.632290272388675	1.5710755087893096
7	1.163005893064212	1.3059554768485648
8	0.8638203457219192	1.108566707049082
9	0.6669725765458138	0.9629392802590789
>10	3.8095607088824313	11.84375548123224
>50	0.7857097509669992	9.22131736320403
>100	1.6290862724742672	46.35782008884621
>500	0.013455579831645864	1.4158178439849896
>1k	0.007688902760940495	2.730880274592631
>5k	0.0019222256902351237	1.8246982667496845
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCTT	7636	0.5887709445085698	TruSeq Adapter, Index 15 (95% over 23bp)
TTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	5581	0.43032093259590465	TruSeq Adapter, Index 15 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	5330	0.4109676708002458	TruSeq Adapter, Index 15 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	5123	0.3950070126659774	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	4655	0.3589220464493704	TruSeq Adapter, Index 14 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	4033	0.31096296741789703	TruSeq Adapter, Index 15 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	3841	0.2961588787136481	TruSeq Adapter, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	3721	0.28690632327349247	TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2677	0.20640909094413848	TruSeq Adapter, Index 15 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2195	0.16924465992618004	TruSeq Adapter, Index 15 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2075	0.1599921044860244	TruSeq Adapter, Index 15 (95% over 21bp)
CTCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	2018	0.1555971406519505	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1845	0.14225803989239277	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGCT	1308	0.10085285429769633	TruSeq Adapter, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6146780997410055	0.0
2	0.0	0.0	0.0	1.2856425784096246	0.0
3	0.0	0.0	0.0	4.6107025850868855	0.0
4	0.0	0.0	0.0	5.389382230004649	0.0
5	0.0	0.0	0.0	6.453426105622547	0.0
6	0.0	0.0	0.0	9.873170596304067	0.0
7	0.0	0.0	0.0	10.288610335567055	0.0
8	0.0	0.0	0.0	11.136067309256642	0.0
9	7.710462866796357E-5	0.0	0.0	11.680040464509124	0.0
10	7.710462866796357E-5	0.0	0.0	13.46508972280115	0.0
11	7.710462866796357E-5	0.0	0.0	15.070716510182823	0.0
12	7.710462866796357E-5	0.0	0.0	15.729961085293912	0.0
13	7.710462866796357E-5	0.0	0.0	16.03028361395563	0.0
14	7.710462866796357E-5	0.0	0.0	16.174700583450726	0.0
15	7.710462866796357E-5	0.0	0.0	16.423363010904907	0.0
16	7.710462866796357E-5	0.0	0.0	17.10072717375297	0.0
17	7.710462866796357E-5	0.0	0.0	18.089362722533597	0.0
18	7.710462866796357E-5	0.0	0.0	19.154408958324176	0.0
19	7.710462866796357E-5	0.0	0.0	19.774792800586614	0.0
20	7.710462866796357E-5	0.0	0.0	20.384690413350203	0.0
21	1.5420925733592713E-4	0.0	0.0	21.46477205173104	0.0
22	1.5420925733592713E-4	0.0	0.0	22.34268535374447	0.0
23	1.5420925733592713E-4	0.0	0.0	23.29006992618774	0.0
24	0.002621557374710761	0.0	0.0	24.02726728088214	0.0
25	0.002621557374710761	0.0	0.0	24.643333263939166	0.0
26	0.002621557374710761	0.0	0.0	25.180906735012208	0.0
27	0.002621557374710761	0.0	0.0	25.686096262044707	0.0
28	0.002621557374710761	0.0	0.0	26.15967289132334	0.0
29	0.002621557374710761	0.0	0.0	26.706421813207868	0.0
30	0.002621557374710761	0.0	0.0	27.383863080684595	0.0
31	0.002621557374710761	0.0	0.0	27.927682026679744	0.0
32	0.002621557374710761	0.0	0.0	28.436572575888302	0.0
33	0.002621557374710761	0.0	0.0	28.92125227169512	0.0
34	0.002698662003378725	0.0	0.0	29.37802009192414	0.0
35	0.002698662003378725	0.0	0.0	30.07689644617056	0.0
36	0.002698662003378725	0.0	0.0	30.58385937966242	0.0
37	0.0027757666320466882	0.0	0.0	31.07378219021866	0.0
38	0.0027757666320466882	0.0	0.0	31.564784465576253	0.0
39	0.0027757666320466882	0.0	0.0	32.03219272456145	0.0
40	0.0027757666320466882	0.0	0.0	32.547251644063444	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGT	55	1.8189894E-12	46.000004	43
CCGGGTA	35	1.0197073E-7	46.000004	5
AAATCGA	35	1.0197073E-7	46.000004	46
CTCAAGT	35	1.0197073E-7	46.000004	17
GGTAACC	35	1.0197073E-7	46.000004	8
CACGGGC	155	0.0	46.000004	4
ACCGAAA	35	1.0197073E-7	46.000004	24
CGCGACA	35	1.0197073E-7	46.000004	38
ACGGGTC	35	1.0197073E-7	46.000004	5
GCCGCGA	35	1.0197073E-7	46.000004	36
CTTATCG	70	0.0	46.000004	1
CGGATGA	35	1.0197073E-7	46.000004	6
TGCGACA	55	1.8189894E-12	46.000004	36
CATCGCG	35	1.0197073E-7	46.000004	1
CAATGAG	70	0.0	46.000004	17
CCGGTCA	35	1.0197073E-7	46.000004	34
TAGGCCA	35	1.0197073E-7	46.000004	19
CGACTTG	35	1.0197073E-7	46.000004	1
CGGTAAG	55	1.8189894E-12	46.000004	22
ATGCCCG	55	1.8189894E-12	46.000004	43
>>END_MODULE