FastQCFastQC Report
Thu 26 May 2016
SRR1545662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545662_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1296732
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC104590.8065660444872187No Hit
ATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG45040.3473346844220703No Hit
ATGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT39360.3035322641841182No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT38360.29582057048025345No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38050.29342994543205536No Hit
ATTGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC34600.26682460215372183No Hit
ATATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC32980.25433165835346083No Hit
ATGTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC29160.22487298840469733No Hit
ATGCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC28500.2197832705601466No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC24700.19047883448546038No Hit
ATTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC21700.16734375337386598No Hit
ATGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC21430.1652615960738225No Hit
ATAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC20910.16125151534781282No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT18220.14050705928441654No Hit
ATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC17630.1359571599991363No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT15350.11837449835432456No Hit
ATCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT14210.10958316753191871No Hit
ATCTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC13940.10750101023187522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT2200.045.0000048
ATCTCGC502.1827873E-1145.0000041
GTCGCGG253.8910828E-545.0000043
GTACCGC253.8910828E-545.00000428
TACCCGG253.8910828E-545.00000427
CCGGCTA502.1827873E-1145.00000442
CGTTGTG253.8910828E-545.0000043
CGCATAT253.8910828E-545.00000434
GGTCCCG253.8910828E-545.00000411
CCGTGAG502.1827873E-1145.00000424
ATGTCGC253.8910828E-545.0000041
GACCCGT253.8910828E-545.00000432
TAGCCCG502.1827873E-1145.0000042
CCGTCGG253.8910828E-545.00000442
CACCTAG253.8910828E-545.00000442
CGTTAAC253.8910828E-545.00000436
CCCGTAT253.8910828E-545.00000434
CCGTAGT253.8910828E-545.00000422
CGTAGCG502.1827873E-1145.0000043
CTACGAT253.8910828E-545.00000436