##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545662_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1296732
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88221775972213	31.0	31.0	34.0	31.0	34.0
2	32.570630631464326	34.0	31.0	34.0	31.0	34.0
3	32.473730886567154	34.0	31.0	34.0	31.0	34.0
4	35.93188029600565	37.0	35.0	37.0	35.0	37.0
5	35.680434353436176	37.0	35.0	37.0	33.0	37.0
6	35.66310155066737	37.0	35.0	37.0	33.0	37.0
7	35.64040912077438	37.0	35.0	37.0	33.0	37.0
8	35.603629739992535	37.0	35.0	37.0	33.0	37.0
9	37.87845059734779	39.0	38.0	39.0	35.0	39.0
10	37.86160286011296	39.0	38.0	39.0	35.0	39.0
11	37.64250515912309	39.0	37.0	39.0	35.0	39.0
12	37.03893248566396	39.0	37.0	39.0	33.0	39.0
13	36.74047528710636	39.0	35.0	39.0	32.0	39.0
14	37.27825873040844	39.0	35.0	41.0	32.0	41.0
15	37.061587899427174	39.0	35.0	41.0	31.0	41.0
16	37.13252931214777	39.0	35.0	41.0	32.0	41.0
17	37.30040363004846	39.0	35.0	41.0	32.0	41.0
18	37.408058102985045	39.0	35.0	41.0	32.0	41.0
19	37.38269202888492	39.0	35.0	41.0	32.0	41.0
20	37.35484741642838	39.0	35.0	41.0	32.0	41.0
21	37.337018751754414	39.0	35.0	41.0	32.0	41.0
22	37.258565378196884	39.0	35.0	41.0	32.0	41.0
23	37.206947156390065	39.0	35.0	41.0	32.0	41.0
24	37.13852823868	39.0	35.0	41.0	32.0	41.0
25	37.08524274869441	39.0	35.0	41.0	32.0	41.0
26	36.993418069423754	39.0	35.0	41.0	32.0	41.0
27	36.88504872248082	39.0	35.0	41.0	31.0	41.0
28	36.81154779861991	38.0	35.0	41.0	31.0	41.0
29	36.779214980427724	38.0	35.0	41.0	31.0	41.0
30	36.750047812500966	38.0	35.0	41.0	31.0	41.0
31	36.74102898671429	38.0	35.0	41.0	31.0	41.0
32	36.629230249581255	38.0	35.0	41.0	31.0	41.0
33	36.50616704145498	38.0	35.0	41.0	31.0	41.0
34	36.401459977852014	38.0	35.0	41.0	30.0	41.0
35	36.34488236582424	38.0	35.0	41.0	30.0	41.0
36	36.27305565066644	38.0	35.0	41.0	30.0	41.0
37	36.13474025473267	38.0	35.0	41.0	30.0	41.0
38	36.04072931029696	38.0	35.0	40.0	30.0	41.0
39	35.987142293087544	38.0	35.0	40.0	30.0	41.0
40	35.91535722107575	38.0	35.0	40.0	30.0	41.0
41	35.91917682296727	38.0	35.0	40.0	30.0	41.0
42	35.778939672962494	38.0	35.0	40.0	29.0	41.0
43	35.695171400104265	38.0	35.0	40.0	29.0	41.0
44	35.62814675661586	38.0	35.0	40.0	29.0	41.0
45	35.60288247687263	38.0	34.0	40.0	29.0	41.0
46	35.55183800507738	38.0	34.0	40.0	29.0	41.0
47	35.394696822473726	37.0	34.0	40.0	28.0	41.0
48	35.39017699879389	37.0	34.0	40.0	28.0	41.0
49	35.380878238525774	37.0	34.0	40.0	28.0	41.0
50	35.28370627084085	37.0	34.0	40.0	28.0	41.0
51	35.179011545947816	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	9.0
12	9.0
13	9.0
14	7.0
15	20.0
16	49.0
17	123.0
18	280.0
19	529.0
20	1026.0
21	1830.0
22	2877.0
23	4388.0
24	6585.0
25	9900.0
26	13383.0
27	16253.0
28	18909.0
29	22656.0
30	28109.0
31	34440.0
32	43039.0
33	55597.0
34	86936.0
35	184002.0
36	91409.0
37	117892.0
38	176987.0
39	379281.0
40	196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.2761310741155458	92.13052504295413	3.179299963292338	3.414043919637982
2	3.4652495658316442	1.9694123380929907	91.79190457241742	2.7734335236579337
3	25.56973993084153	15.656126323712224	45.558141543510914	13.215992201935325
4	25.092000505887107	16.633043682117815	45.63309920631248	12.641856605682591
5	22.86170156979237	13.121369720188905	49.12495411542246	14.891974594596261
6	19.790365318354137	14.75300987405262	50.31324899825099	15.143375809342254
7	22.017888044715484	17.185200951314535	47.402393092790184	13.394517911179795
8	22.543362853696834	18.610630415536903	45.639422795149656	13.206583935616612
9	82.11234086920042	3.2259557102007204	10.06908135219922	4.592622068399638
10	83.93291751880882	3.357748555599769	9.282488594405013	3.4268453311863976
11	77.16243603150073	6.269838332053192	10.368372184846214	6.199353451599868
12	39.27087478368699	30.101054034295444	15.255195368048295	15.37287581396927
13	33.03242304500853	23.016629496303015	29.884741025902034	14.066206432786421
14	30.55542702732716	26.22253480287369	25.910828143363467	17.311210026435685
15	20.098833066508732	35.844106569437635	30.26670121505446	13.790359148999176
16	15.240003331451678	39.77884404796057	28.44219160165709	16.538961018930664
17	13.352874765178926	27.519564566926707	41.73630326081257	17.391257407081802
18	16.327814845318848	24.51801914350845	40.36223367665794	18.791932334514765
19	16.32380476459284	22.76021568064951	29.35194010790202	31.564039446855634
20	18.679650074186494	24.432033758710357	35.768454854202716	21.11986131290043
21	22.790059935283466	26.965402257366982	29.568175999358388	20.676361807991164
22	23.535395131761998	25.11189667564308	28.89972638910739	22.45298180348754
23	19.098163691495234	28.874663384569825	30.586967854575963	21.44020506935897
24	26.03791685560316	22.52470055493348	26.43476061360405	25.002621975859313
25	20.69317330026559	28.09130953813124	24.331396155874923	26.884121005728247
26	20.33396260753957	22.999895120965625	35.40461714525438	21.26152512624043
27	21.309106276393273	25.735849813222778	30.94818358766499	22.006860322718957
28	17.84162031938751	31.913224937766632	27.255516174506372	22.989638568339487
29	16.159699922574593	32.42304500852914	25.88568802188887	25.531567047007396
30	16.318406579000133	27.41376012930968	33.22729754490519	23.040535746784997
31	16.516905574937613	23.823349774664308	33.70388021580404	25.95586443459404
32	17.85457596481	32.11365185713008	26.818340258434283	23.213431919625645
33	21.723532696038966	29.166473874324073	23.888976288084198	25.221017141552764
34	24.221581637531887	26.035603347492003	28.406177992060037	21.33663702291607
35	21.93722372857306	30.239247585468704	22.797617395113253	25.025911290844988
36	17.332648534932428	27.53413966802701	25.93920717619369	29.19400462084687
37	19.02713899248264	26.1555201845871	30.1298957687479	24.687445054182362
38	23.101303893171448	27.32546123640043	25.032157762745115	24.54107710768301
39	19.899562901200866	32.84795933161208	29.076478408799964	18.175999358387084
40	18.41390510915131	28.149147240910228	30.31235444178134	23.124593208157123
41	19.682710074248185	29.86908628768319	26.5216714016466	23.92653223642202
42	23.838927395946115	25.215310488211905	26.43738258946336	24.508379526378622
43	18.730084551009767	25.680788320177182	28.828470339283673	26.76065678952937
44	18.808589592915112	25.638836706428158	26.940570603640534	28.612003097016192
45	18.519786663705375	24.844146670244893	28.893711268018375	27.74235539803136
46	21.513311925671612	26.819882597175056	27.6326951135624	24.034110363590937
47	16.850590561503843	31.471807590157415	26.085652239630086	25.591949608708664
48	18.81044039940404	29.185753108583732	25.47665978783588	26.527146704176346
49	18.959353204825668	26.755798422495936	30.098123590687976	24.186724781990417
50	18.99135673369671	23.819108343127184	33.42286609723521	23.766668825940904
51	18.56482295493595	23.759265599985195	29.100616010093066	28.575295434985794
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	725.0
1	792.5
2	860.0
3	3037.5
4	5215.0
5	3641.5
6	2068.0
7	2256.0
8	2444.0
9	3046.5
10	3649.0
11	3887.5
12	4126.0
13	4122.0
14	4118.0
15	4636.0
16	5154.0
17	5032.0
18	4910.0
19	4563.0
20	4216.0
21	4551.5
22	4887.0
23	6109.5
24	7332.0
25	8875.0
26	12330.0
27	14242.0
28	15960.5
29	17679.0
30	23810.0
31	29941.0
32	31751.5
33	33562.0
34	38593.0
35	43624.0
36	49597.5
37	55571.0
38	70879.0
39	86187.0
40	102207.0
41	118227.0
42	120117.5
43	122008.0
44	128721.5
45	135435.0
46	140259.5
47	145084.0
48	151130.5
49	157177.0
50	135368.0
51	113559.0
52	87952.5
53	62346.0
54	48599.5
55	34853.0
56	30254.5
57	25656.0
58	21164.0
59	16672.0
60	14087.5
61	11503.0
62	9023.5
63	6544.0
64	4642.0
65	2740.0
66	2198.0
67	1656.0
68	1395.5
69	1135.0
70	866.5
71	598.0
72	463.5
73	329.0
74	188.5
75	38.0
76	28.0
77	94.0
78	160.0
79	91.0
80	22.0
81	14.0
82	6.0
83	6.5
84	7.0
85	5.0
86	3.0
87	3.5
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1296732.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.44485811278456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.44372137142174	11.591012918098434
2	12.152702143560692	4.24004329162698
3	5.985047017190437	3.1322488803969484
4	3.640240784232217	2.5401393590921044
5	2.4030665671714004	2.096057764994067
6	1.6054131926184965	1.6803723214573278
7	1.126842436485468	1.3760324493967402
8	0.8126225217327019	1.1340867672704136
9	0.5841329800973539	0.917110526111671
>10	3.403643714646397	11.194097949200751
>50	0.6323971547683133	7.955259792624957
>100	1.1750862851867887	44.6200322242349
>500	0.025757749259574355	2.9327200590478153
>1k	0.008881982503301501	3.7805012136155143
>5k	0.0	0.0
>10k+	4.440991251650751E-4	0.8102844828313591
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	10459	0.8065660444872187	No Hit
ATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTG	4504	0.3473346844220703	No Hit
ATGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	3936	0.3035322641841182	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	3836	0.29582057048025345	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3805	0.29342994543205536	No Hit
ATTGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	3460	0.26682460215372183	No Hit
ATATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	3298	0.25433165835346083	No Hit
ATGTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	2916	0.22487298840469733	No Hit
ATGCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	2850	0.2197832705601466	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2470	0.19047883448546038	No Hit
ATTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	2170	0.16734375337386598	No Hit
ATGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	2143	0.1652615960738225	No Hit
ATAGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	2091	0.16125151534781282	No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1822	0.14050705928441654	No Hit
ATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	1763	0.1359571599991363	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1535	0.11837449835432456	No Hit
ATCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	1421	0.10958316753191871	No Hit
ATCTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	1394	0.10750101023187522	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3268215791697899	0.0
2	7.711693703864793E-5	0.0	0.0	1.4405443838819432	0.0
3	7.711693703864793E-5	0.0	0.0	1.9466628416665896	0.0
4	7.711693703864793E-5	0.0	0.0	2.579407310068696	0.0
5	7.711693703864793E-5	0.0	0.0	4.944198184358834	0.0
6	7.711693703864793E-5	0.0	0.0	5.512472893396631	0.0
7	7.711693703864793E-5	0.0	0.0	6.383662931122236	0.0
8	7.711693703864793E-5	0.0	0.0	8.99461106843974	0.0
9	7.711693703864793E-5	0.0	0.0	9.273620146645568	0.0
10	1.5423387407729585E-4	0.0	0.0	9.895645360799302	0.0
11	2.3135081111594376E-4	0.0	0.0	10.332898393808435	0.0
12	3.084677481545917E-4	0.0	0.0	11.713831385359503	0.0
13	3.855846851932396E-4	0.0	0.0	12.96543927349676	0.0
14	3.855846851932396E-4	0.0	0.0	13.466930715059087	0.0
15	3.855846851932396E-4	0.0	0.0	13.725041103327442	0.0
16	3.855846851932396E-4	0.0	0.0	13.861846549634002	0.0
17	3.855846851932396E-4	0.0	0.0	14.071064799819855	0.0
18	3.855846851932396E-4	0.0	0.0	14.603017431512448	0.0
19	4.6270162223188753E-4	0.0	0.0	15.413130855103445	0.0
20	6.169354963091834E-4	0.0	0.0	16.276763432999264	0.0
21	6.169354963091834E-4	0.0	0.0	16.776712535820817	0.0
22	6.169354963091834E-4	0.0	0.0	17.334499341421356	0.0
23	6.940524333478313E-4	0.0	0.0	18.1921939151652	0.0
24	7.711693703864792E-4	0.0	0.0	18.94508657147352	0.0
25	8.482863074251272E-4	0.0	0.0	19.71517630474146	0.0
26	8.482863074251272E-4	0.0	0.0	20.32308911941712	0.0
27	8.482863074251272E-4	0.0	0.0	20.847561408216965	0.0
28	8.482863074251272E-4	0.0	0.0	21.3438860149977	0.0
29	8.482863074251272E-4	0.0	0.0	21.792166770003362	0.0
30	8.482863074251272E-4	0.0	0.0	22.26682151747624	0.0
31	8.482863074251272E-4	0.0	0.0	22.75566578136423	0.0
32	8.482863074251272E-4	0.0	0.0	23.34938907962478	0.0
33	8.482863074251272E-4	0.0	0.0	23.871008041754195	0.0
34	9.254032444637751E-4	0.0	0.0	24.39555744749108	0.0
35	9.254032444637751E-4	0.0	0.0	24.87776965479374	0.0
36	9.254032444637751E-4	0.0	0.0	25.358593757229713	0.0
37	0.001002520181502423	0.0	0.0	25.945607881967902	0.0
38	0.001002520181502423	0.0	0.0	26.49205849782376	0.0
39	0.001002520181502423	0.0	0.0	27.00812504048639	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	220	0.0	45.000004	8
ATCTCGC	50	2.1827873E-11	45.000004	1
GTCGCGG	25	3.8910828E-5	45.000004	3
GTACCGC	25	3.8910828E-5	45.000004	28
TACCCGG	25	3.8910828E-5	45.000004	27
CCGGCTA	50	2.1827873E-11	45.000004	42
CGTTGTG	25	3.8910828E-5	45.000004	3
CGCATAT	25	3.8910828E-5	45.000004	34
GGTCCCG	25	3.8910828E-5	45.000004	11
CCGTGAG	50	2.1827873E-11	45.000004	24
ATGTCGC	25	3.8910828E-5	45.000004	1
GACCCGT	25	3.8910828E-5	45.000004	32
TAGCCCG	50	2.1827873E-11	45.000004	2
CCGTCGG	25	3.8910828E-5	45.000004	42
CACCTAG	25	3.8910828E-5	45.000004	42
CGTTAAC	25	3.8910828E-5	45.000004	36
CCCGTAT	25	3.8910828E-5	45.000004	34
CCGTAGT	25	3.8910828E-5	45.000004	22
CGTAGCG	50	2.1827873E-11	45.000004	3
CTACGAT	25	3.8910828E-5	45.000004	36
>>END_MODULE