FastQCFastQC Report
Thu 26 May 2016
SRR1545661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545661_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1170764
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT85480.7301215274812003Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC68090.5815860412516954No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT64780.5533139044248029No Hit
TTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC62490.5337540272847474No Hit
GTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC62480.5336686129740922No Hit
ATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC53960.4608956202958069No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC47550.4061450471657823No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC37860.3233785801408311No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC32390.27665695221240144No Hit
CTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC27330.23343731102083765No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC24380.20824008937753466No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT23340.19935700106938717No Hit
TCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC21970.18765524050961593No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT16500.1409336125811863No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT15570.1329900816902467No Hit
TACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC13280.11343020455019115No Hit
GACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC12740.10881783177480688No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC12530.10702413125104632No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA551.8189894E-1246.00000427
AGCGTAT351.0195981E-746.00000418
CGTATAG351.0195981E-746.00000420
CGCGGCC351.0195981E-746.00000412
CGGCCGC351.0195981E-746.00000421
TAGGTTA551.8189894E-1246.00000417
GTCCTCG351.0195981E-746.00000440
TAGCGCG551.8189894E-1246.0000041
TAGCGAA351.0195981E-746.00000416
GGCCGGT551.8189894E-1246.0000049
TGGCGAC551.8189894E-1246.00000429
GGCCGAC551.8189894E-1246.00000415
ATTCGAT351.0195981E-746.00000429
CGATTAG551.8189894E-1246.0000041
CCGCTGC351.0195981E-746.00000440
ACGATCA351.0195981E-746.00000439
TGCGCTA351.0195981E-746.00000427
CGCTCCA351.0195981E-746.00000421
ACCGTAA351.0195981E-746.00000419
AGTAGCG551.8189894E-1246.0000041
Produced by FastQC (version 0.11.5)