##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545661_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1170764
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.495625933151345	33.0	31.0	34.0	31.0	34.0
2	32.862089199872905	34.0	31.0	34.0	31.0	34.0
3	32.925343621771766	34.0	31.0	34.0	31.0	34.0
4	36.33047992592871	37.0	37.0	37.0	35.0	37.0
5	36.2950620278724	37.0	37.0	37.0	35.0	37.0
6	36.29843162242775	37.0	37.0	37.0	35.0	37.0
7	36.444531092517366	37.0	37.0	37.0	35.0	37.0
8	36.513422858919476	37.0	37.0	37.0	35.0	37.0
9	38.30622994899058	39.0	39.0	39.0	37.0	39.0
10	37.989271108438594	39.0	38.0	39.0	35.0	39.0
11	37.805690130547234	39.0	38.0	39.0	35.0	39.0
12	37.400429121496735	39.0	37.0	39.0	35.0	39.0
13	37.29312910202227	39.0	37.0	39.0	35.0	39.0
14	38.5836718587179	40.0	37.0	41.0	35.0	41.0
15	38.644772131702034	40.0	37.0	41.0	35.0	41.0
16	38.72501802241955	40.0	37.0	41.0	35.0	41.0
17	38.68070251562228	40.0	37.0	41.0	35.0	41.0
18	38.723894824234435	40.0	37.0	41.0	35.0	41.0
19	38.70196042925816	40.0	37.0	41.0	35.0	41.0
20	38.6145653607388	40.0	37.0	41.0	35.0	41.0
21	38.58909737573072	40.0	37.0	41.0	35.0	41.0
22	38.65100225152123	40.0	37.0	41.0	35.0	41.0
23	38.62215527638362	40.0	37.0	41.0	35.0	41.0
24	38.60761178170836	40.0	37.0	41.0	35.0	41.0
25	38.50438089999351	40.0	37.0	41.0	35.0	41.0
26	38.41260834805307	40.0	36.0	41.0	35.0	41.0
27	38.393465292749006	40.0	36.0	41.0	35.0	41.0
28	38.386191410053605	40.0	36.0	41.0	35.0	41.0
29	38.40594432353574	40.0	36.0	41.0	35.0	41.0
30	38.35205472665712	40.0	36.0	41.0	35.0	41.0
31	38.29145412739032	40.0	36.0	41.0	35.0	41.0
32	38.22229586833897	40.0	36.0	41.0	34.0	41.0
33	38.182359553248986	40.0	36.0	41.0	34.0	41.0
34	38.13683799638527	40.0	36.0	41.0	34.0	41.0
35	38.07510736578849	40.0	35.0	41.0	34.0	41.0
36	37.98544881803677	40.0	35.0	41.0	34.0	41.0
37	37.90485529107489	40.0	35.0	41.0	34.0	41.0
38	37.80648277535011	40.0	35.0	41.0	34.0	41.0
39	37.799808501115514	40.0	35.0	41.0	34.0	41.0
40	37.69274678756778	40.0	35.0	41.0	33.0	41.0
41	37.59787113372123	40.0	35.0	41.0	33.0	41.0
42	37.58529473062035	40.0	35.0	41.0	33.0	41.0
43	37.51342969206433	40.0	35.0	41.0	33.0	41.0
44	37.42929317949647	40.0	35.0	41.0	33.0	41.0
45	37.28915050343195	39.0	35.0	41.0	33.0	41.0
46	37.33907260558063	39.0	35.0	41.0	33.0	41.0
47	37.348975540758	39.0	35.0	41.0	33.0	41.0
48	37.27873166581822	39.0	35.0	41.0	33.0	41.0
49	37.29855632732131	39.0	35.0	41.0	33.0	41.0
50	37.2021090501587	39.0	35.0	41.0	33.0	41.0
51	37.07806270093717	39.0	35.0	41.0	33.0	41.0
52	36.708403230710886	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	0.0
12	1.0
13	1.0
14	2.0
15	7.0
16	23.0
17	39.0
18	92.0
19	183.0
20	352.0
21	592.0
22	1022.0
23	1509.0
24	2275.0
25	3177.0
26	4276.0
27	5522.0
28	7043.0
29	9133.0
30	11572.0
31	15475.0
32	19862.0
33	27457.0
34	58230.0
35	146081.0
36	61464.0
37	81081.0
38	148375.0
39	564917.0
40	995.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.807421478624214	14.16459679320512	47.32960699167381	12.69837473649685
2	23.496024817982104	14.267350208923405	50.566894779818995	11.669730193275502
3	23.660105708750866	13.05839605590879	46.66192332528161	16.61957491005873
4	20.02965584865951	14.580564486096259	50.803663248955374	14.58611641628885
5	22.809208346003125	16.993433347796824	46.30753935037292	13.889818955827135
6	20.82341103757888	18.134397709529846	47.00118896720432	14.041002285686954
7	83.05986518205206	2.258525202346502	8.316535185571132	6.3650744300303055
8	86.10035839844751	1.6853951778496776	8.42099688750252	3.793249536200293
9	77.27526640723494	3.7185120143769366	10.691565507651415	8.314656070736715
10	37.75321072393753	29.534987409930608	14.399742390439062	18.312059475692795
11	30.914941012877062	20.900710988721894	33.3792292895921	14.805118708808948
12	29.085195650019983	26.30188492300754	25.994478818959244	18.618440608013227
13	20.034780707298825	32.21238439173053	34.30537666002713	13.447458240943522
14	14.9463085643221	39.94255033465327	28.58424071802686	16.52690038299777
15	12.856818282762367	27.52459078003765	39.81733295523265	19.80125798196733
16	15.852554400374455	26.727931504556	37.85519541085991	19.564318684209628
17	15.53395902163032	24.20368238176097	27.657837104659865	32.604521491948844
18	17.580400490619798	25.39717654454698	33.855926557359126	23.166496407474092
19	20.449894257083407	28.645653607387995	30.27834815556338	20.62610397996522
20	22.320723903365668	24.104430952779552	27.949441561236938	25.625403582617846
21	18.183938009709898	30.25451756289056	31.31023844258963	20.25130598480992
22	25.649575832533284	22.21344352918265	26.43641246228958	25.70056817599448
23	20.510367589027336	28.906679740750484	22.269646145593818	28.313306524628363
24	20.146417211325254	22.271696089049545	34.36644789214564	23.21543880747956
25	24.072315171973173	25.890187945649167	27.778442111305097	22.259054771072563
26	17.04792767799488	31.67692207823268	25.73806505837214	25.537085185400304
27	16.216761021008505	28.565962055546635	24.905104700862	30.312172222582863
28	17.081410087771744	25.972783584052806	31.719287576317683	25.226518751857764
29	15.343228865937114	22.17671537560089	33.53263339152895	28.947422366933047
30	17.504381754136613	32.10459153168358	25.05662968796444	25.33439702621536
31	19.956114127185327	27.245115155573625	22.030144418516457	30.768626298724595
32	24.898869456184165	25.586112999716427	26.69820732444797	22.81681021965144
33	20.583823896190864	30.150226689580478	21.503821436258715	27.762127977969943
34	15.401566840114661	27.83071566942612	24.2504040096894	32.51731348076982
35	19.287832560618536	24.192749349997094	28.710055997622064	27.809362091762303
36	21.302926977597533	24.47564154688733	24.590780037650628	29.630651437864508
37	20.66812782080761	32.979746558657425	27.16585067528554	19.186274945249426
38	16.259553590646792	26.71588808675361	32.144480014759594	24.88007830784001
39	20.039649323006174	29.576584179219722	25.37548130964054	25.008285188133563
40	24.778264449538934	23.382338370499948	26.90952232900909	24.92987485095203
41	18.803106347649912	24.87461179195807	28.136755144503933	28.185526715888088
42	18.455299274661673	26.215958126488342	24.850439542042633	30.47830305680735
43	18.291303798203568	22.961245818969495	29.355959014797172	29.391491368029765
44	25.086353868072468	24.384333648796854	24.237506448780454	26.291806034350216
45	16.76930619663741	27.943291730869756	26.54770730907339	28.739694763419443
46	18.832403456204666	26.69880522462255	24.481535134322545	29.987256184850235
47	19.548175379495785	24.646726411129826	30.4694199684992	25.335678240875186
48	17.486700991830975	21.745202278170495	35.708648369782466	25.059448360216063
49	18.29779528581337	21.17275556815891	27.86274603592184	32.66670311010588
50	17.31399325568603	23.986644618385945	31.939656497808265	26.759705628119757
51	20.797872158692957	24.062919597801095	26.637819406814696	28.501388836691255
52	18.29121838389291	21.551482621604354	29.9745294525626	30.182769541940136
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	495.0
1	369.0
2	243.0
3	214.5
4	186.0
5	269.0
6	352.0
7	458.0
8	564.0
9	955.5
10	1347.0
11	1487.5
12	1628.0
13	1675.5
14	1861.5
15	2000.0
16	2318.5
17	2637.0
18	2274.5
19	1912.0
20	2214.0
21	2516.0
22	3092.0
23	3668.0
24	4330.0
25	4992.0
26	6138.0
27	7284.0
28	7990.0
29	8696.0
30	11876.5
31	15057.0
32	18124.5
33	21192.0
34	23880.0
35	26568.0
36	29874.0
37	33180.0
38	35985.5
39	51400.5
40	64010.0
41	74964.0
42	85918.0
43	87724.0
44	89530.0
45	95608.5
46	101687.0
47	114129.5
48	126572.0
49	145451.5
50	164331.0
51	158829.0
52	153327.0
53	121388.0
54	89449.0
55	67242.0
56	45035.0
57	37025.0
58	29015.0
59	24199.0
60	19383.0
61	15242.5
62	11102.0
63	9384.5
64	5721.5
65	3776.0
66	3063.0
67	2350.0
68	1675.5
69	1001.0
70	880.0
71	759.0
72	583.5
73	408.0
74	307.5
75	207.0
76	154.0
77	101.0
78	79.5
79	58.0
80	40.0
81	22.0
82	18.0
83	14.0
84	9.0
85	4.0
86	3.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1170764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.242488621300364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.13763832356092	10.714075217484766
2	12.937231463540291	4.4614013260244505
3	6.520557038371659	3.3729189161599
4	3.9799341893260407	2.744958798919142
5	2.7259236269098484	2.3500853559763444
6	1.9490245854057004	2.016362054189547
7	1.319140002393345	1.5921679536818574
8	1.0371461777329904	1.4306384934549004
9	0.8323580138651837	1.291673122462674
>10	4.574656417204018	14.845925722813167
>50	0.804557331231402	9.999348909127697
>100	1.1531163226384802	36.97710224189497
>500	0.01881426374420878	2.1968175997900987
>1k	0.006931570853129551	2.614958220929177
>5k	0.0029706732227698074	3.391566067091324
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCTT	8548	0.7301215274812003	Illumina Single End Adapter 2 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	6809	0.5815860412516954	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	6478	0.5533139044248029	No Hit
TTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	6249	0.5337540272847474	No Hit
GTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	6248	0.5336686129740922	No Hit
ATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	5396	0.4608956202958069	No Hit
GCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	4755	0.4061450471657823	No Hit
GGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	3786	0.3233785801408311	No Hit
AGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	3239	0.27665695221240144	No Hit
CTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2733	0.23343731102083765	No Hit
ACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2438	0.20824008937753466	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	2334	0.19935700106938717	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	2197	0.18765524050961593	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	1650	0.1409336125811863	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	1557	0.1329900816902467	No Hit
TACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1328	0.11343020455019115	No Hit
GACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1274	0.10881783177480688	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	1253	0.10702413125104632	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7556604063671244	0.0
2	0.0	0.0	0.0	1.7409144797755995	0.0
3	0.0	0.0	0.0	6.10165669596947	0.0
4	0.0	0.0	0.0	7.096220929239369	0.0
5	0.0	0.0	0.0	8.606175113003133	0.0
6	0.0	0.0	0.0	13.15781831351152	0.0
7	0.0	0.0	0.0	13.667485505191483	0.0
8	0.0	0.0	0.0	14.772917513691914	0.0
9	0.0	0.0	0.0	15.459306914117619	0.0
10	0.0	0.0	0.0	17.943582139525986	0.0
11	0.0	0.0	0.0	20.0579279854864	0.0
12	0.0	0.0	0.0	20.910619048757905	0.0
13	0.0	0.0	0.0	21.31744741040893	0.0
14	0.0	0.0	0.0	21.51133789559638	0.0
15	0.0	0.0	0.0	21.829762445719204	0.0
16	0.0	0.0	0.0	22.650166899563022	0.0
17	8.541431065526443E-5	0.0	0.0	23.984082189066285	0.0
18	8.541431065526443E-5	0.0	0.0	25.36036297665456	0.0
19	8.541431065526443E-5	0.0	0.0	26.172994728228748	0.0
20	8.541431065526443E-5	0.0	0.0	27.030127335654324	0.0
21	8.541431065526443E-5	0.0	0.0	28.331328944176622	0.0
22	1.7082862131052886E-4	0.0	0.0	29.419678090546004	0.0
23	1.7082862131052886E-4	0.0	0.0	30.593697790502613	0.0
24	2.562429319657933E-4	0.0	0.0	31.493793796187788	0.0
25	2.562429319657933E-4	0.0	0.0	32.25970391983355	0.0
26	2.562429319657933E-4	0.0	0.0	32.96351783963292	0.0
27	2.562429319657933E-4	0.0	0.0	33.58072164842786	0.0
28	2.562429319657933E-4	0.0	0.0	34.21389793331534	0.0
29	2.562429319657933E-4	0.0	0.0	34.86595078085763	0.0
30	2.562429319657933E-4	0.0	0.0	35.65919348391307	0.0
31	2.562429319657933E-4	0.0	0.0	36.36719270493455	0.0
32	2.562429319657933E-4	0.0	0.0	37.04709061775046	0.0
33	2.562429319657933E-4	0.0	0.0	37.66053619687657	0.0
34	2.562429319657933E-4	0.0	0.0	38.260657143540456	0.0
35	2.562429319657933E-4	0.0	0.0	39.02272362320673	0.0
36	2.562429319657933E-4	0.0	0.0	39.69595921979152	0.0
37	2.562429319657933E-4	0.0	0.0	40.32939174761096	0.0
38	2.562429319657933E-4	0.0	0.0	40.986911111035184	0.0
39	2.562429319657933E-4	0.0	0.0	41.587544543563006	0.0
40	2.562429319657933E-4	0.0	0.0	42.21363144066609	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	55	1.8189894E-12	46.000004	27
AGCGTAT	35	1.0195981E-7	46.000004	18
CGTATAG	35	1.0195981E-7	46.000004	20
CGCGGCC	35	1.0195981E-7	46.000004	12
CGGCCGC	35	1.0195981E-7	46.000004	21
TAGGTTA	55	1.8189894E-12	46.000004	17
GTCCTCG	35	1.0195981E-7	46.000004	40
TAGCGCG	55	1.8189894E-12	46.000004	1
TAGCGAA	35	1.0195981E-7	46.000004	16
GGCCGGT	55	1.8189894E-12	46.000004	9
TGGCGAC	55	1.8189894E-12	46.000004	29
GGCCGAC	55	1.8189894E-12	46.000004	15
ATTCGAT	35	1.0195981E-7	46.000004	29
CGATTAG	55	1.8189894E-12	46.000004	1
CCGCTGC	35	1.0195981E-7	46.000004	40
ACGATCA	35	1.0195981E-7	46.000004	39
TGCGCTA	35	1.0195981E-7	46.000004	27
CGCTCCA	35	1.0195981E-7	46.000004	21
ACCGTAA	35	1.0195981E-7	46.000004	19
AGTAGCG	55	1.8189894E-12	46.000004	1
>>END_MODULE