FastQCFastQC Report
Thu 26 May 2016
SRR1545660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545660_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2344742
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC77530.3306547159559559No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.21524756241838122No Hit
ATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT36930.15750133703409586No Hit
ATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG34760.14824658747103092No Hit
ATGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC30010.12798849510948326No Hit
ATGTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC28940.12342509325119778No Hit
ATTGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC26540.11318942553167896No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT25730.10973488767634136Illumina Single End Adapter 1 (95% over 21bp)
ATATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC25620.10926575290586343No Hit
ATGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC23910.10197283965570625No Hit
ATGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA23600.10065073257526842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA351.2126475E-745.0000041
TTCACGA351.2126475E-745.0000042
TAAGTCG351.2126475E-745.00000436
CCGTACT351.2126475E-745.00000444
GAACGTA207.034789E-445.00000418
TCCGTTA302.166471E-645.00000438
CGCACCA207.034789E-445.00000432
TATACGA207.034789E-445.00000440
ACGACTA207.034789E-445.00000421
ACACGTA750.045.01
CGACGAA253.892051E-545.043
ATTCGGT551.8189894E-1245.01
TCGATGT850.045.02
ATCGTAC1100.045.01
TAACACG253.892051E-545.035
CGTACGA253.892051E-545.019
CGTAAAC253.892051E-545.034
TCGGACA1350.043.3333322
GTCGATA850.042.3529411
ATAGCCG800.042.1875041