##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545660_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2344742
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.893654397797285	31.0	31.0	34.0	31.0	34.0
2	32.571763119353854	34.0	31.0	34.0	31.0	34.0
3	32.55190421803337	34.0	31.0	34.0	31.0	34.0
4	36.019743750058645	37.0	35.0	37.0	35.0	37.0
5	35.79671835963189	37.0	35.0	37.0	35.0	37.0
6	35.80284440676202	37.0	35.0	37.0	35.0	37.0
7	35.77523156065784	37.0	35.0	37.0	35.0	37.0
8	35.74059448758115	37.0	35.0	37.0	33.0	37.0
9	37.92369565606792	39.0	38.0	39.0	35.0	39.0
10	37.87344321891278	39.0	38.0	39.0	35.0	39.0
11	37.687621068757245	39.0	38.0	39.0	35.0	39.0
12	37.22275798360758	39.0	37.0	39.0	33.0	39.0
13	36.97767771464835	39.0	37.0	39.0	33.0	39.0
14	37.65928021078652	40.0	36.0	41.0	33.0	41.0
15	37.54588479244198	40.0	36.0	41.0	32.0	41.0
16	37.40981694361256	40.0	36.0	41.0	32.0	41.0
17	37.55141759733054	40.0	35.0	41.0	32.0	41.0
18	37.62862609191118	40.0	35.0	41.0	33.0	41.0
19	37.627803400118225	40.0	35.0	41.0	33.0	41.0
20	37.61141524312696	40.0	35.0	41.0	33.0	41.0
21	37.57726308480848	39.0	35.0	41.0	33.0	41.0
22	37.49535130091072	39.0	35.0	41.0	32.0	41.0
23	37.439284578004745	39.0	35.0	41.0	32.0	41.0
24	37.42842069617894	39.0	35.0	41.0	32.0	41.0
25	37.40545996105328	39.0	35.0	41.0	32.0	41.0
26	37.29221978366916	39.0	35.0	41.0	32.0	41.0
27	37.19549485615048	39.0	35.0	41.0	32.0	41.0
28	37.12241986538391	39.0	35.0	41.0	32.0	41.0
29	37.08194974116555	39.0	35.0	41.0	32.0	41.0
30	37.016676888118184	39.0	35.0	41.0	31.0	41.0
31	36.903129640702474	39.0	35.0	41.0	31.0	41.0
32	36.78230312759357	39.0	35.0	41.0	31.0	41.0
33	36.66445050244334	39.0	35.0	41.0	31.0	41.0
34	36.60693287363812	39.0	35.0	41.0	31.0	41.0
35	36.51062121120362	39.0	35.0	41.0	30.0	41.0
36	36.36762594775886	38.0	35.0	41.0	30.0	41.0
37	36.28610866355446	38.0	35.0	41.0	30.0	41.0
38	36.19144025227509	38.0	35.0	41.0	30.0	41.0
39	36.08936505594219	38.0	35.0	40.0	30.0	41.0
40	35.99686234135781	38.0	35.0	40.0	30.0	41.0
41	35.924105935749004	38.0	35.0	40.0	30.0	41.0
42	35.77505371593122	38.0	35.0	40.0	29.0	41.0
43	35.65977450824014	38.0	35.0	40.0	29.0	41.0
44	35.610185257056	38.0	34.0	40.0	29.0	41.0
45	35.59227411800531	37.0	34.0	40.0	29.0	41.0
46	35.54080150396078	37.0	34.0	40.0	29.0	41.0
47	35.471846369451306	37.0	34.0	40.0	29.0	41.0
48	35.42808590454728	37.0	34.0	40.0	29.0	41.0
49	35.38251116753997	37.0	34.0	40.0	29.0	41.0
50	35.25162384603509	36.0	34.0	40.0	28.0	41.0
51	35.08224529607095	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	18.0
11	25.0
12	30.0
13	22.0
14	56.0
15	69.0
16	126.0
17	297.0
18	674.0
19	1313.0
20	2274.0
21	3687.0
22	5682.0
23	7999.0
24	11572.0
25	16316.0
26	21957.0
27	26876.0
28	31793.0
29	38339.0
30	47240.0
31	59090.0
32	73243.0
33	95524.0
34	158459.0
35	261144.0
36	177290.0
37	244743.0
38	365046.0
39	693533.0
40	294.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.9536904273476567	91.61187030385433	3.3734201886604156	3.061019080137602
2	3.246924395093362	2.638072760243984	91.29251747100534	2.822485373657315
3	28.129619378166126	16.06829237502463	42.73020229944275	13.071885947366491
4	27.212887388036723	15.642787138201134	43.965263555649194	13.179061918112952
5	24.903933993590766	14.140660251746247	46.24086573277572	14.71454002188727
6	23.29906659240121	14.485303713585546	47.01898972253664	15.196639971476605
7	23.120667433773097	17.333378256541657	44.37797420782329	15.167980101861952
8	26.61909071445814	19.152640247839635	42.68068725684958	11.547581780852648
9	83.21132986059874	3.898509942671731	8.683087520929808	4.207072675799726
10	84.07833356505748	3.9358274812324767	8.324796502131152	3.6610424515788944
11	77.52784741348941	6.379763743729587	9.803978433448115	6.288410409332881
12	44.47713223885613	20.7147310876847	16.473582168102077	18.334554505357094
13	35.79105931484146	23.810807329761655	22.213659327977236	18.184474027419647
14	29.90934610289746	23.034261338774158	26.789642527834616	20.26675003049376
15	24.4544175862419	24.92346705948885	31.499414434509216	19.122700919760042
16	19.901635233215423	29.68237017121713	31.315215064173373	19.10077953139407
17	20.493598016327596	23.239657071012505	36.882735925743646	19.384008986916257
18	25.069112081414502	20.493171530172617	33.18893933746229	21.248777050950597
19	24.905469343748692	20.497095202798434	28.2237875211857	26.373647932267176
20	25.470648796328128	22.105374493227824	30.164128931882484	22.259847778561564
21	27.05760377900852	23.700560658699338	27.275197015279296	21.96663854701285
22	27.295071270101356	24.203217240958708	26.03979457014887	22.461916918791065
23	27.0793545729125	23.50484616217904	28.137296128955764	21.278503135952697
24	27.975274038678883	21.06722189477563	26.915413294938205	24.04209077160728
25	27.476413183198833	23.299706321633682	25.19377398451514	24.030106510652345
26	26.91426178231976	22.769541382378105	28.48206753664156	21.834129298660578
27	28.203273537131164	23.077379089042633	25.54536917068061	23.17397820314559
28	23.069659689637493	26.188851481314362	25.44877005657765	25.29271877247049
29	22.392314378298337	24.751422544569934	28.303369837704956	24.55289323942677
30	22.921370453550967	25.363686068659153	27.914329167132244	23.800614310657632
31	22.536637293143553	25.356904938794973	26.87340440867268	25.233053359388798
32	24.361870090611248	25.226656067064095	28.229587732893428	22.18188610943123
33	25.755029764468755	26.332961153082085	25.290628990311088	22.621380092138068
34	27.296436025797295	24.32741000928887	24.398803791632513	23.977350173281327
35	27.47116740349258	24.946838500781748	24.322420121275602	23.259573974450067
36	23.154317191401017	24.71261230446676	28.227114113194546	23.90595639093768
37	24.10704461301073	25.591472324033944	27.08170024676489	23.21978281619044
38	28.041379392700776	25.99680476572689	23.1567481624844	22.805067679087934
39	24.466060658272852	26.977381733256795	25.632329697681023	22.924227910789334
40	25.558121106714516	26.61729947260722	25.547800141764	22.27677927891427
41	24.092160246201928	25.788679522096675	24.210168965284883	25.90899126641652
42	26.976272869253847	22.767835437758187	26.759532605293035	23.496359087694938
43	22.83001711915426	23.58587853162523	27.16601655960443	26.418087789616084
44	21.934268247849868	25.145879589310894	27.064981989489674	25.854870173349564
45	22.20316776856473	23.60370565290339	27.58175526347888	26.611371315053
46	24.36357603523117	23.903525419854297	27.22947769946544	24.503420845449096
47	21.450163813332125	25.522125675234204	26.93243009252191	26.09528041891176
48	22.593615843448873	24.86090154055329	26.525093165900554	26.02038945009728
49	22.41316955127686	25.018914660973362	29.225049067232128	23.34286672051765
50	22.77640780947328	24.59353736999636	29.724677597791143	22.90537722273922
51	22.565083919680713	24.5032502509871	26.599685594406548	26.331980234925634
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	645.0
1	1189.0
2	1733.0
3	5125.5
4	8518.0
5	5879.0
6	3240.0
7	3243.5
8	3247.0
9	3359.0
10	3471.0
11	3802.0
12	4133.0
13	4148.5
14	4164.0
15	4255.5
16	4347.0
17	4641.5
18	4936.0
19	5439.5
20	5943.0
21	6334.5
22	6726.0
23	7503.0
24	8280.0
25	9609.0
26	14778.5
27	18619.0
28	23944.0
29	29269.0
30	32251.0
31	35233.0
32	38500.0
33	41767.0
34	49778.0
35	57789.0
36	67958.5
37	78128.0
38	89598.5
39	101069.0
40	119913.0
41	138757.0
42	157171.5
43	175586.0
44	194568.5
45	213551.0
46	214601.0
47	215651.0
48	195315.5
49	174980.0
50	166405.0
51	157830.0
52	151518.5
53	145207.0
54	144626.5
55	144046.0
56	141207.0
57	138368.0
58	126475.0
59	114582.0
60	103724.0
61	92866.0
62	82964.5
63	73063.0
64	60884.5
65	48706.0
66	40150.0
67	31594.0
68	25988.5
69	20383.0
70	16477.5
71	12572.0
72	9859.0
73	7146.0
74	5152.5
75	2651.5
76	2144.0
77	1814.5
78	1485.0
79	925.0
80	365.0
81	346.5
82	328.0
83	230.5
84	133.0
85	79.0
86	25.0
87	18.0
88	11.0
89	10.0
90	9.0
91	4.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2344742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.725570329056765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.43262084217207	11.390442731714797
2	10.801726431360969	3.3972661734319654
3	4.45018605375386	2.0994514129708177
4	2.41262662021925	1.5175971837604938
5	1.5498880439786782	1.2186436718875464
6	0.9832102111001576	0.9276924793741368
7	0.6812085174581523	0.7498674715028467
8	0.49764232183026325	0.6260567464525526
9	0.39164119865284724	0.5542903091854297
>10	2.943552012835375	10.051238291099969
>50	0.9910201221676271	11.57550773903249
>100	1.8494313188885367	52.68870863689536
>500	0.010345707359385049	1.1047653170100153
>1k	0.004356087309214758	1.550456176957088
>5k	5.445109136518447E-4	0.5480156587245465
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	7753	0.3306547159559559	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5047	0.21524756241838122	No Hit
ATGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCT	3693	0.15750133703409586	No Hit
ATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	3476	0.14824658747103092	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	3001	0.12798849510948326	No Hit
ATGTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	2894	0.12342509325119778	No Hit
ATTGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	2654	0.11318942553167896	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	2573	0.10973488767634136	Illumina Single End Adapter 1 (95% over 21bp)
ATATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	2562	0.10926575290586343	No Hit
ATGGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTC	2391	0.10197283965570625	No Hit
ATGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAA	2360	0.10065073257526842	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1172836926194865	0.0
2	4.264861549799509E-5	0.0	0.0	0.5637720482679971	0.0
3	4.264861549799509E-5	0.0	0.0	0.7831138777741858	0.0
4	4.264861549799509E-5	0.0	0.0	1.075640731474934	0.0
5	4.264861549799509E-5	0.0	0.0	2.180751656258983	0.0
6	4.264861549799509E-5	0.0	0.0	2.4370271867864353	0.0
7	4.264861549799509E-5	0.0	0.0	2.8498657848070277	0.0
8	4.264861549799509E-5	0.0	0.0	4.126040306353535	0.0
9	4.264861549799509E-5	0.0	0.0	4.285503479700538	0.0
10	4.264861549799509E-5	0.0	0.0	4.599738478689766	0.0
11	4.264861549799509E-5	0.0	0.0	4.83068073161141	0.0
12	4.264861549799509E-5	0.0	0.0	5.52350749037634	0.0
13	8.529723099599018E-5	0.0	0.0	6.122549943661179	0.0
14	1.2794584649398525E-4	0.0	0.0	6.369144238470587	0.0
15	1.2794584649398525E-4	0.0	0.0	6.511761208695882	0.0
16	1.2794584649398525E-4	0.0	0.0	6.61859599051836	0.0
17	2.1324307748997543E-4	0.0	0.0	6.757587828426326	0.0
18	2.1324307748997543E-4	0.0	0.0	7.051394140592014	0.0
19	2.1324307748997543E-4	0.0	0.0	7.417532504642302	0.0
20	2.558916929879705E-4	0.0	0.0	7.840180284227433	0.0
21	2.9854030848596564E-4	0.0	0.0	8.109463642481774	0.0
22	2.9854030848596564E-4	0.0	0.0	8.409539301125667	0.0
23	2.9854030848596564E-4	0.0	0.0	8.866433918955689	0.0
24	3.411889239839607E-4	0.0	0.0	9.244556543960913	0.0
25	3.838375394819558E-4	0.0	0.0	9.651125795503301	0.0
26	3.838375394819558E-4	0.0	0.0	9.968985926809857	0.0
27	3.838375394819558E-4	0.0	0.0	10.25822883711726	0.0
28	4.2648615497995087E-4	0.0	0.0	10.5275548439871	0.0
29	4.2648615497995087E-4	0.0	0.0	10.814110891518128	0.0
30	4.2648615497995087E-4	0.0	0.0	11.09384316056948	0.0
31	5.11783385975941E-4	0.0	0.0	11.424839065449419	0.0
32	5.11783385975941E-4	0.0	0.0	11.798057099672373	0.0
33	5.11783385975941E-4	0.0	0.0	12.122996901151598	0.0
34	5.544320014739362E-4	0.0	0.0	12.433564119207999	0.0
35	5.970806169719313E-4	0.0	0.0	12.757565651146267	0.0
36	5.970806169719313E-4	0.0	0.0	13.11125061947114	0.0
37	6.823778479679214E-4	0.0	0.0	13.493638106026165	0.0
38	7.250264634659165E-4	0.0	0.0	13.826169361064032	0.0
39	8.103236944619067E-4	0.0	0.0	14.176954223535041	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGA	35	1.2126475E-7	45.000004	1
TTCACGA	35	1.2126475E-7	45.000004	2
TAAGTCG	35	1.2126475E-7	45.000004	36
CCGTACT	35	1.2126475E-7	45.000004	44
GAACGTA	20	7.034789E-4	45.000004	18
TCCGTTA	30	2.166471E-6	45.000004	38
CGCACCA	20	7.034789E-4	45.000004	32
TATACGA	20	7.034789E-4	45.000004	40
ACGACTA	20	7.034789E-4	45.000004	21
ACACGTA	75	0.0	45.0	1
CGACGAA	25	3.892051E-5	45.0	43
ATTCGGT	55	1.8189894E-12	45.0	1
TCGATGT	85	0.0	45.0	2
ATCGTAC	110	0.0	45.0	1
TAACACG	25	3.892051E-5	45.0	35
CGTACGA	25	3.892051E-5	45.0	19
CGTAAAC	25	3.892051E-5	45.0	34
TCGGACA	135	0.0	43.333332	2
GTCGATA	85	0.0	42.35294	11
ATAGCCG	80	0.0	42.187504	1
>>END_MODULE