FastQCFastQC Report
Thu 26 May 2016
SRR1545659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545659_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1915844
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT58340.30451331110466195Illumina Single End Adapter 1 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC54640.2852006739588401No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT53700.2802942201974691Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC47910.25007255288008834No Hit
GCCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC43910.22919402623595655No Hit
TTCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC39270.2049749353287637No Hit
GGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC39010.20361783109689516No Hit
ATCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC37960.19813721785281058No Hit
AGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC19770.1031921179386213No Hit
ACCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC19450.10152183580709076No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC19210.10026912420844286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC206.3125126E-446.00000412
TAGACGT206.3125126E-446.00000443
CGACATA206.3125126E-446.00000410
ATGTCGC206.3125126E-446.00000411
ACGATTG405.6152203E-946.00000421
TATTACG206.3125126E-446.00000416
TTACGCA206.3125126E-446.00000427
ATCGAGT206.3125126E-446.00000438
AACGTAT301.8620576E-646.07
CCGTATG253.4180157E-546.035
CCCGTAT253.4180157E-546.034
TACGCCG253.4180157E-546.09
TCTAACG551.8189894E-1246.017
CGTACGA501.6370905E-1146.017
TTCGATA301.8620576E-646.039
CGATAGA253.4180157E-546.039
TAAGGCG453.110472E-1045.9999961
GCGATTA700.042.71428723
ATAAGCG554.7293724E-1141.8181833
CGTTATG950.041.1578941
Produced by FastQC (version 0.11.5)