FastQCFastQC Report
Thu 26 May 2016
SRR1545658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545658_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3368596
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC94480.2804729329370456No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70170.2083063685879815No Hit
ATGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT50970.15130932887173174No Hit
ATGCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC47810.14192856608509896No Hit
ATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG40360.11981252723686664No Hit
ATGTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC35210.10452425877131007No Hit
ATTGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC34320.10188220849279642No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAT351.2128658E-745.034
CGTAAGT207.0352294E-445.028
ACGTATA253.892416E-545.013
TACGCAT1150.041.086962
ATCGGTT3650.040.0684931
GCGATAC900.040.011
ACTACCG403.460882E-739.37512
ATCGATT3550.038.661971
TACGCTA1050.038.571432
AACCGTA356.251883E-638.57142640
CCCGTAT356.251883E-638.57142620
CGAACCC2500.037.80000319
TAATACG301.1403901E-437.49999612
CGCGATA301.1403901E-437.49999616
CGAATCG301.1403901E-437.49999645
ATCCGAT1150.037.1739161
CGCAATA552.752131E-936.8181822
ATCGCGC1650.036.818181
CGTTTTT44050.036.0102161
CGCATTA250.002107685236.042