##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545658_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3368596
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.786896083709653	31.0	31.0	34.0	31.0	34.0
2	32.43449021491446	33.0	31.0	34.0	31.0	34.0
3	32.44423997416134	34.0	31.0	34.0	31.0	34.0
4	35.951282967740866	37.0	35.0	37.0	35.0	37.0
5	35.733831839733824	37.0	35.0	37.0	35.0	37.0
6	35.743603863449344	37.0	35.0	37.0	35.0	37.0
7	35.72629427809093	37.0	35.0	37.0	33.0	37.0
8	35.680505765606796	37.0	35.0	37.0	33.0	37.0
9	37.79743459886552	39.0	37.0	39.0	35.0	39.0
10	37.66880267031131	39.0	38.0	39.0	35.0	39.0
11	37.51383068791865	39.0	37.0	39.0	35.0	39.0
12	37.14510466675137	39.0	37.0	39.0	33.0	39.0
13	36.962788057695256	39.0	37.0	39.0	33.0	39.0
14	37.853766673118415	40.0	36.0	41.0	33.0	41.0
15	37.830260440848356	40.0	36.0	41.0	33.0	41.0
16	37.73429108150695	40.0	36.0	41.0	32.0	41.0
17	37.801716798333786	40.0	36.0	41.0	33.0	41.0
18	37.849241048793026	40.0	36.0	41.0	33.0	41.0
19	37.840245312884065	40.0	36.0	41.0	33.0	41.0
20	37.76651103308322	40.0	36.0	41.0	33.0	41.0
21	37.74762245160892	40.0	36.0	41.0	33.0	41.0
22	37.67583378950756	40.0	36.0	41.0	32.0	41.0
23	37.608797255592535	40.0	36.0	41.0	32.0	41.0
24	37.60243792963003	40.0	35.0	41.0	32.0	41.0
25	37.55232476675743	40.0	35.0	41.0	32.0	41.0
26	37.4469695980165	40.0	35.0	41.0	32.0	41.0
27	37.349514456467915	40.0	35.0	41.0	32.0	41.0
28	37.27620795132453	39.0	35.0	41.0	32.0	41.0
29	37.22234396763518	39.0	35.0	41.0	31.0	41.0
30	37.13650642582251	39.0	35.0	41.0	31.0	41.0
31	37.04200563083255	39.0	35.0	41.0	31.0	41.0
32	36.93199184467357	39.0	35.0	41.0	31.0	41.0
33	36.82544537843066	39.0	35.0	41.0	31.0	41.0
34	36.766183893824014	39.0	35.0	41.0	30.0	41.0
35	36.68616539353487	39.0	35.0	41.0	30.0	41.0
36	36.546862253591705	39.0	35.0	41.0	30.0	41.0
37	36.45385881833262	39.0	35.0	41.0	30.0	41.0
38	36.379674202546106	39.0	35.0	41.0	30.0	41.0
39	36.31834182549644	39.0	35.0	41.0	30.0	41.0
40	36.25303123319033	39.0	35.0	41.0	30.0	41.0
41	36.17002751294605	38.0	35.0	41.0	30.0	41.0
42	36.041524718309944	38.0	35.0	40.0	29.0	41.0
43	35.928576772043904	38.0	35.0	40.0	29.0	41.0
44	35.847590509517914	38.0	35.0	40.0	29.0	41.0
45	35.83207276859558	38.0	35.0	40.0	29.0	41.0
46	35.790689652306185	38.0	35.0	40.0	29.0	41.0
47	35.72945464519937	38.0	35.0	40.0	29.0	41.0
48	35.65655869685768	38.0	35.0	40.0	29.0	41.0
49	35.608904125042	38.0	34.0	40.0	29.0	41.0
50	35.50819035586339	37.0	34.0	40.0	28.0	41.0
51	35.303082946129486	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	14.0
10	27.0
11	47.0
12	63.0
13	78.0
14	101.0
15	156.0
16	283.0
17	573.0
18	1061.0
19	2070.0
20	3425.0
21	5337.0
22	8239.0
23	11956.0
24	17080.0
25	23730.0
26	31517.0
27	38621.0
28	45733.0
29	54430.0
30	66164.0
31	81690.0
32	101627.0
33	131194.0
34	206138.0
35	302343.0
36	257478.0
37	359808.0
38	549050.0
39	1068123.0
40	432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.7519773816747395	89.44420761646693	3.82628252245149	3.9775324794068507
2	3.957910061046204	3.581165565713431	88.51248413285535	3.9484402403850147
3	30.960554486201374	17.162610179433806	38.97804901507928	12.898786319285543
4	28.70759806162567	16.460002921098287	39.90790228332516	14.924496733950882
5	26.111056357010458	15.560073098703436	42.51655585888008	15.812314685406026
6	24.838508387470625	15.129893878636677	43.75739922507775	16.274198508814948
7	24.55284041185111	18.137348616456233	40.84476143770283	16.465049533989827
8	26.842785540326	19.831793423729057	41.435749493260694	11.889671542684251
9	78.76254677022713	5.40575361367169	10.42472887814389	5.406970737957297
10	78.03399398443743	6.212944502694891	10.983448297154066	4.769613215713609
11	70.44427411301325	9.0210580312985	12.599344059067933	7.935323796620312
12	39.20956980296836	23.254554716564407	19.287620124229797	18.248255356237436
13	31.07938737681812	25.61256974715876	25.14180982225236	18.16623305377077
14	26.200618892856255	23.956033908488877	28.767029349913138	21.07631784874173
15	23.020866853727785	24.048357238445927	32.96165524153089	19.969120666295396
16	19.677634242871513	28.367189179112014	32.62442275654308	19.330753821473397
17	19.82235328902605	24.220031134632944	36.25439203751355	19.70322353882745
18	24.409665035522217	23.026893103239452	32.6330019984587	19.93043986277963
19	25.086742369818165	23.02003564689859	28.11278051746187	23.78044146582137
20	24.427595354266288	23.237841522105946	30.3864280548929	21.948135068734867
21	26.061480806840592	24.729323433264184	28.02787867705121	21.18131708284401
22	25.932109401067983	24.606275136585094	27.543077293923048	21.918538168423876
23	26.51294485892639	23.39081326463607	29.377253906375238	20.718987970062305
24	27.229148286110888	22.126814851053673	27.667936434051455	22.97610042878398
25	27.34602190348739	23.97535946726767	25.79585085299632	22.88276777624862
26	26.285609791141475	23.041943884039522	27.984982467473095	22.68746385734591
27	27.666719309765845	23.813956912612852	26.222141212540773	22.297182565080526
28	23.835538604213742	25.784540502927626	25.851452652677853	24.528468240180775
29	22.3882293988356	26.01089593409242	27.70771561802009	23.89315904905189
30	22.530425138544366	25.066674662084736	29.15650912130751	23.246391078063382
31	24.192749739060424	24.7223175471324	27.841154000064122	23.243778713743055
32	23.238702414893325	25.647836665483187	28.14424763313855	22.969213286484933
33	24.41690840931949	24.892507145410136	27.75295701829486	22.937627426975514
34	27.1210913983155	24.452501873183962	26.161106882511287	22.265299845989247
35	26.09621337791768	24.0658719537754	26.417356073568932	23.420558594737987
36	22.943564618612623	25.15772149583981	28.38028068667184	23.518433198875734
37	23.63652394053784	26.066022758442987	28.78617679294282	21.511276508076364
38	26.72698061744418	25.5210776240309	25.290863018301984	22.461078740222927
39	24.314877771035768	26.573326097875793	27.14688849597874	21.9649076351097
40	23.766221891850492	25.649231905517905	27.378290540035078	23.206255662596522
41	23.101434544243357	25.763552530490447	26.065132179697414	25.069880745568778
42	24.536394391016316	24.493735669103685	27.870216553127776	23.099653386752227
43	23.36813319258231	24.625333521740213	28.14534601359142	23.861187272086056
44	22.30721641894724	25.751173485927076	27.339431620770192	24.602178474355487
45	21.37291025697353	24.14994258735687	28.293805490477336	26.183341665192266
46	23.45440058706951	24.692305043406808	27.22834676523988	24.624947604283804
47	21.530720810687896	25.37264783310317	27.706320377985367	25.390310978223567
48	21.72240304269197	25.477795496996375	27.032745986755312	25.767055473556344
49	22.27509621219048	25.217034040294532	29.356384677770798	23.15148506974419
50	22.374306684446577	24.643263840484284	29.64347757938322	23.338951895685916
51	22.36109643305401	24.376090216814365	27.89996188322969	25.362851466901937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	850.0
1	2072.0
2	3294.0
3	8028.5
4	12763.0
5	9155.5
6	5548.0
7	5747.0
8	5946.0
9	6133.0
10	6320.0
11	6796.5
12	7273.0
13	7260.5
14	7248.0
15	7923.5
16	8599.0
17	9843.0
18	11087.0
19	11071.0
20	11055.0
21	14172.0
22	17289.0
23	18452.5
24	19616.0
25	23744.0
26	34708.5
27	41545.0
28	45836.5
29	50128.0
30	58291.0
31	66454.0
32	77191.5
33	87929.0
34	100470.0
35	113011.0
36	118075.5
37	123140.0
38	136616.0
39	150092.0
40	166249.5
41	182407.0
42	196258.5
43	210110.0
44	233157.0
45	256204.0
46	277534.5
47	298865.0
48	291957.0
49	285049.0
50	256501.5
51	227954.0
52	216114.5
53	204275.0
54	205769.0
55	207263.0
56	197817.0
57	188371.0
58	172922.0
59	157473.0
60	140405.5
61	123338.0
62	103672.5
63	84007.0
64	73150.5
65	62294.0
66	50273.5
67	38253.0
68	32822.5
69	27392.0
70	21855.0
71	16318.0
72	13161.0
73	10004.0
74	8372.0
75	4725.5
76	2711.0
77	1865.5
78	1020.0
79	924.5
80	829.0
81	646.5
82	464.0
83	286.0
84	108.0
85	81.5
86	55.0
87	40.0
88	25.0
89	15.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3368596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.38850095724779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66353854230677	12.072349719148537
2	10.188934918127327	3.339627393181302
3	3.9533946244289195	1.943706347604948
4	2.1276164699976174	1.3947377822084848
5	1.3212261952152378	1.082645838251289
6	0.8951704670300032	0.8802301233492695
7	0.650722908544298	0.7465061106706906
8	0.5080831465458471	0.6661376906822468
9	0.3786383788353309	0.5584783890594215
>10	3.197047517981956	11.599057258581237
>50	1.3578382384421546	16.476531275078024
>100	1.7464727989353435	46.389313543067864
>500	0.00711799920581733	0.7682370005963192
>1k	0.003650256002983246	1.437498131586143
>5k	5.475384004474869E-4	0.6449433969341938
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	9448	0.2804729329370456	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7017	0.2083063685879815	No Hit
ATGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	5097	0.15130932887173174	No Hit
ATGCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	4781	0.14192856608509896	No Hit
ATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	4036	0.11981252723686664	No Hit
ATGTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	3521	0.10452425877131007	No Hit
ATTGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	3432	0.10188220849279642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.937191637109348E-5	0.0	0.0	0.10185252253461086	0.0
2	8.905787455664021E-5	0.0	0.0	0.49240098842366375	0.0
3	8.905787455664021E-5	0.0	0.0	0.6714963741570672	0.0
4	8.905787455664021E-5	0.0	0.0	0.9394418327398121	0.0
5	8.905787455664021E-5	0.0	0.0	1.9965588037271313	0.0
6	8.905787455664021E-5	0.0	0.0	2.2584780127982103	0.0
7	8.905787455664021E-5	0.0	0.0	2.6868760753738354	0.0
8	8.905787455664021E-5	0.0	0.0	3.883517049833224	0.0
9	1.1874383274218696E-4	0.0	0.0	4.042277554209528	0.0
10	1.4842979092773368E-4	0.0	0.0	4.314883708227404	0.0
11	1.7811574911328042E-4	0.0	0.0	4.530967797859999	0.0
12	1.7811574911328042E-4	0.0	0.0	5.168533121810986	0.0
13	2.0780170729882717E-4	0.0	0.0	5.6953995076880695	0.0
14	2.0780170729882717E-4	0.0	0.0	5.91097893603151	0.0
15	2.3748766548437392E-4	0.0	0.0	6.046940624521314	0.0
16	2.6717362366992066E-4	0.0	0.0	6.166218804510841	0.0
17	2.6717362366992066E-4	0.0	0.0	6.320288927493828	0.0
18	2.9685958185546736E-4	0.0	0.0	6.614803318652638	0.0
19	5.046612891542946E-4	0.0	0.0	6.9946054676785225	0.0
20	5.046612891542946E-4	0.0	0.0	7.380047948759661	0.0
21	5.046612891542946E-4	0.0	0.0	7.649002729920714	0.0
22	5.937191637109347E-4	0.0	0.0	7.94194376529569	0.0
23	6.234051218964815E-4	0.0	0.0	8.362890652366742	0.0
24	6.530910800820282E-4	0.0	0.0	8.751123613517324	0.0
25	6.530910800820282E-4	0.0	0.0	9.112698584217283	0.0
26	6.530910800820282E-4	0.0	0.0	9.407391091125204	0.0
27	6.530910800820282E-4	0.0	0.0	9.68219400604881	0.0
28	7.421489546386684E-4	0.0	0.0	9.946191232192877	0.0
29	7.718349128242152E-4	0.0	0.0	10.220311370078216	0.0
30	7.718349128242152E-4	0.0	0.0	10.495411144583677	0.0
31	7.718349128242152E-4	0.0	0.0	10.801473373476664	0.0
32	7.718349128242152E-4	0.0	0.0	11.152183283480714	0.0
33	7.718349128242152E-4	0.0	0.0	11.459284520910195	0.0
34	8.608927873808554E-4	0.0	0.0	11.775380603669896	0.0
35	8.905787455664022E-4	0.0	0.0	12.079810104862679	0.0
36	9.202647037519489E-4	0.0	0.0	12.409888273927773	0.0
37	9.499506619374957E-4	0.0	0.0	12.772027277833258	0.0
38	0.001039008536494136	0.0	0.0	13.085303194565332	0.0
39	0.001039008536494136	0.0	0.0	13.418587447114465	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGAT	35	1.2128658E-7	45.0	34
CGTAAGT	20	7.0352294E-4	45.0	28
ACGTATA	25	3.892416E-5	45.0	13
TACGCAT	115	0.0	41.08696	2
ATCGGTT	365	0.0	40.068493	1
GCGATAC	90	0.0	40.0	11
ACTACCG	40	3.460882E-7	39.375	12
ATCGATT	355	0.0	38.66197	1
TACGCTA	105	0.0	38.57143	2
AACCGTA	35	6.251883E-6	38.571426	40
CCCGTAT	35	6.251883E-6	38.571426	20
CGAACCC	250	0.0	37.800003	19
TAATACG	30	1.1403901E-4	37.499996	12
CGCGATA	30	1.1403901E-4	37.499996	16
CGAATCG	30	1.1403901E-4	37.499996	45
ATCCGAT	115	0.0	37.173916	1
CGCAATA	55	2.752131E-9	36.81818	22
ATCGCGC	165	0.0	36.81818	1
CGTTTTT	4405	0.0	36.010216	1
CGCATTA	25	0.0021076852	36.0	42
>>END_MODULE