FastQCFastQC Report
Thu 26 May 2016
SRR1545657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545657_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2690180
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT76080.28280635496509526Illumina Single End Adapter 2 (95% over 21bp)
GTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC74170.27570645830390533No Hit
GCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC72110.26804897813529205No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCTT71530.26589298857325533Illumina Single End Adapter 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC66530.24730687165914547No Hit
GGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC56550.21020898229858223No Hit
ATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC47140.17522991026622753No Hit
AGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC44020.163632173311823No Hit
TTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC42080.15642075994914836No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC31970.11883963154881828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.020253E-746.00000425
TAATACG206.3129707E-446.010
TAACGTG206.3129707E-446.036
CGCTATC453.110472E-1046.037
TCGTACC206.3129707E-446.028
TCGGTAC253.418386E-546.024
CGTCAAC650.042.46153643
GCGATAC508.731149E-1041.49
TAAGACG355.3757994E-639.4285741
ATACCCG355.3757994E-639.42857412
AATGCGT657.2759576E-1238.92307341
CGTAGCA301.0019234E-438.33333225
GTCGTTA301.0019234E-438.3333321
CTACCGT301.0019234E-438.33333229
CGTAAGG850.037.882352
CACGCGA552.219167E-937.6363619
GGACTAA15450.037.3656968
AGGGCGA27650.036.9330946
AGCGTAC250.001892062136.80000318
CGTATAT250.001892062136.80000324
Produced by FastQC (version 0.11.5)