SRR1545656_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545656_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1808130
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	10132	0.5603579388650152	Illumina PCR Primer Index 3 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6235	0.34483140039709537	No Hit
ATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTG	6012	0.33249821638930827	Illumina PCR Primer Index 3 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	3828	0.21171044117403062	TruSeq Adapter, Index 14 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	3808	0.21060432601638154	Illumina PCR Primer Index 3 (95% over 24bp)
ATGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	3638	0.20120234717636454	Illumina PCR Primer Index 3 (95% over 21bp)
ATATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	3614	0.19987500898718566	TruSeq Adapter, Index 14 (95% over 22bp)
ATTGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	3524	0.19489749077776486	TruSeq Adapter, Index 14 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	3236	0.17896943250761838	No Hit
ATGGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2644	0.1462284238412061	No Hit
ATGTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2586	0.14302068988402383	No Hit
ATTTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2514	0.1390386753164872	TruSeq Adapter, Index 14 (95% over 22bp)
ATAGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2369	0.1310193404235315	TruSeq Adapter, Index 14 (95% over 22bp)
ATACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTC	2104	0.11636331458468141	TruSeq Adapter, Index 14 (95% over 22bp)
ATCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCT	1983	0.10967131788090458	Illumina PCR Primer Index 3 (95% over 21bp)
ATCGTTATGGGCGATATTTAGGTGGGAGAGAAGACACACGGGGAAGAAAAC	1967	0.10878642575478532	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	75	0.0	45.000004	17
ATCCGGT	75	0.0	45.000004	13
ATGACCG	75	0.0	45.000004	1
TAGTGCG	75	0.0	45.000004	3
ATCGCGA	145	0.0	45.000004	1
TGATCGA	40	6.8193913E-9	45.0	16
ACGCGAG	70	0.0	45.0	45
ACAACGC	40	6.8193913E-9	45.0	13
CTTCGAC	25	3.8916958E-5	45.0	43
CGGAATA	20	7.0343615E-4	45.0	34
CGGGTCA	35	1.2124474E-7	45.0	8
TAATACG	45	3.8562575E-10	45.0	40
AACCGGT	20	7.0343615E-4	45.0	15
CGGAACG	40	6.8193913E-9	45.0	39
GCCCATA	20	7.0343615E-4	45.0	34
GAAGCGT	35	1.2124474E-7	45.0	24
CAAACGA	20	7.0343615E-4	45.0	3
ACCGGCC	35	1.2124474E-7	45.0	28
AGATCCG	50	2.1827873E-11	45.0	33
ACGCCTA	20	7.0343615E-4	45.0	24

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content