SRR1545655_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545655_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1461268
Sequences flagged as poor quality	0
Sequence length	52
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCTT	10208	0.6985713777349535	Illumina PCR Primer Index 3 (96% over 25bp)
TTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	6506	0.4452297593596794	Illumina PCR Primer Index 3 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	6223	0.4258630175984145	Illumina PCR Primer Index 3 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	5504	0.376659175455837	TruSeq Adapter, Index 14 (96% over 26bp)
ATCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	5087	0.34812231568747143	Illumina PCR Primer Index 3 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	4810	0.32916617622503197	TruSeq Adapter, Index 14 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	4749	0.324991719520307	TruSeq Adapter, Index 14 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	4240	0.2901589578366186	TruSeq Adapter, Index 14 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	3151	0.21563464059980783	Illumina PCR Primer Index 3 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	2832	0.19380428504559055	TruSeq Adapter, Index 14 (96% over 26bp)
ACCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2560	0.17519031416550557	Illumina PCR Primer Index 3 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2489	0.17033152029607163	Illumina PCR Primer Index 3 (95% over 23bp)
CTCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGC	2478	0.16957874941489173	Illumina PCR Primer Index 3 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATTGCGTCTCGTATGCCGTCTTCTGCT	1822	0.12468623140998092	TruSeq Adapter, Index 14 (96% over 26bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	35	1.0198164E-7	46.000004	14
ACGCGAG	20	6.312019E-4	46.000004	43
CGGAATA	35	1.0198164E-7	46.000004	32
CGGGTCA	40	5.6134013E-9	46.000004	6
AGCCGTA	20	6.312019E-4	46.000004	12
AGCGTAA	55	1.8189894E-12	46.000004	45
TAATACG	40	5.6134013E-9	46.000004	38
CGGAACG	20	6.312019E-4	46.000004	37
AACCGCA	40	5.6134013E-9	46.000004	17
CCTTAAG	20	6.312019E-4	46.000004	41
ACACGTC	20	6.312019E-4	46.000004	22
CGAACAA	20	6.312019E-4	46.000004	35
GGTGTCG	35	1.0198164E-7	46.000004	15
CTCCGTC	40	5.6134013E-9	46.000004	33
CTACTCG	20	6.312019E-4	46.000004	30
CACGGAC	35	1.0198164E-7	46.000004	11
CGAAACC	20	6.312019E-4	46.000004	22
TCCGATA	35	1.0198164E-7	46.000004	36
GCGGTCC	40	5.6134013E-9	46.000004	29
CGTGCGG	105	0.0	46.000004	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content