FastQCFastQC Report
Thu 26 May 2016
SRR1545654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545654_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2384204
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC101850.4271865998043791Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67540.28328112862825494No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT52930.222002815195344No Hit
ATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG48460.2032544195043713No Hit
ATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT43010.18039563728607114No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT40270.1689033320974212Illumina Single End Adapter 2 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC35550.1491063684147833No Hit
ATTGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC34550.14491209644812272No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC34370.14415712749412382Illumina Single End Adapter 2 (95% over 21bp)
ATGTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC32620.13681715155246785No Hit
ATATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC30920.12968688920914484No Hit
ATGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC29140.12222108510848903No Hit
ATAGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC24830.10414377293218198No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG600.045.00000444
GGCGTAA302.1664873E-645.00000424
AGGCGTA302.1664873E-645.00000414
CCCGTAG302.1664873E-645.00000424
ACGTAAC302.1664873E-645.00000441
TACCCGC207.034813E-445.028
CGAGTTA502.1827873E-1145.035
GTCGACA406.8212103E-945.039
ACGTTAC502.1827873E-1145.025
CCTAGCG253.8920713E-545.023
TAGAACG850.045.02
CACGCTA253.8920713E-545.028
CGATTGA207.034813E-445.019
CGCGACA351.2126657E-745.034
ATCGTAC502.1827873E-1145.01
TACGATG406.8212103E-945.019
CGCTAGC253.8920713E-545.028
TATTCGA351.2126657E-745.038
CGAATAC253.8920713E-545.018
GTATCGC700.045.05
Produced by FastQC (version 0.11.5)