FastQCFastQC Report
Thu 26 May 2016
SRR1545653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545653_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1915090
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT82720.4319379245884006Illumina Single End Adapter 2 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC61530.3212903832195876Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT61520.3212381663524952Illumina Single End Adapter 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC59150.3088627688515944Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC52510.2741907691022354Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC50460.26348631134829176Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC46880.2447926729292096Illumina Single End Adapter 2 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC44960.23476703444746724Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC31830.16620628795513528Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC28220.1473559989347759Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC21300.11122192690682943Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT20110.10500811972283287Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG206.312512E-446.00000411
ATCTAAC206.312512E-446.00000423
ACGTTAC206.312512E-446.00000423
ATTACGC351.0200529E-746.00000414
TCTAACG206.312512E-446.00000433
ATAAGCG405.6152203E-946.00000433
AACGACA206.312512E-446.00000436
GCGATGA206.312512E-446.00000441
TTACGAC351.0200529E-746.00000419
TGAATCG351.0200529E-746.00000436
ATACCGG206.312512E-446.00000424
GTAACGC351.0200529E-746.00000415
TACGTCC405.6152203E-946.00000422
GTACGCT351.0200529E-746.00000433
AACGTTG253.4180146E-546.010
CGTGGTA301.8620576E-646.011
GTCGACA253.4180146E-546.037
TTTACGA253.4180146E-546.026
CTATACG253.4180146E-546.012
CGCATGT301.8620576E-646.044
Produced by FastQC (version 0.11.5)