##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545653_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1915090
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53517693685414	33.0	31.0	34.0	31.0	34.0
2	32.94887185458647	34.0	31.0	34.0	31.0	34.0
3	33.03869739803351	34.0	33.0	34.0	31.0	34.0
4	36.41229028400754	37.0	37.0	37.0	35.0	37.0
5	36.38559127769452	37.0	37.0	37.0	35.0	37.0
6	36.390254243925874	37.0	37.0	37.0	35.0	37.0
7	36.473957881875	37.0	37.0	37.0	35.0	37.0
8	36.504585685268054	37.0	37.0	37.0	35.0	37.0
9	38.302478734680875	39.0	39.0	39.0	37.0	39.0
10	38.07334067850597	39.0	39.0	39.0	35.0	39.0
11	37.919964596964114	39.0	38.0	39.0	35.0	39.0
12	37.64955119602734	39.0	37.0	39.0	35.0	39.0
13	37.61267198930599	39.0	37.0	39.0	35.0	39.0
14	38.99327394534983	41.0	38.0	41.0	35.0	41.0
15	38.997882083870735	41.0	38.0	41.0	35.0	41.0
16	39.060303171130336	41.0	38.0	41.0	35.0	41.0
17	39.004830582374716	41.0	38.0	41.0	35.0	41.0
18	38.995131299312305	41.0	38.0	41.0	35.0	41.0
19	38.95534831261194	41.0	38.0	41.0	35.0	41.0
20	38.87729976136892	41.0	38.0	41.0	35.0	41.0
21	38.83794077562934	41.0	38.0	41.0	35.0	41.0
22	38.93904202935632	41.0	38.0	41.0	35.0	41.0
23	38.93648131419411	41.0	38.0	41.0	35.0	41.0
24	38.889290842728016	41.0	38.0	41.0	35.0	41.0
25	38.8047590452668	41.0	38.0	41.0	35.0	41.0
26	38.70057125252599	40.0	38.0	41.0	35.0	41.0
27	38.62396493115206	40.0	38.0	41.0	35.0	41.0
28	38.533425583131866	40.0	37.0	41.0	35.0	41.0
29	38.53217551133367	40.0	37.0	41.0	35.0	41.0
30	38.484663383966286	40.0	37.0	41.0	35.0	41.0
31	38.39081609741579	40.0	37.0	41.0	35.0	41.0
32	38.38350155867348	40.0	37.0	41.0	35.0	41.0
33	38.306071255136835	40.0	37.0	41.0	34.0	41.0
34	38.23728858695936	40.0	36.0	41.0	34.0	41.0
35	38.1495788709669	40.0	36.0	41.0	34.0	41.0
36	38.04208366186445	40.0	36.0	41.0	34.0	41.0
37	37.96847719950498	40.0	36.0	41.0	34.0	41.0
38	37.912167052201205	40.0	35.0	41.0	34.0	41.0
39	37.84745416664491	40.0	35.0	41.0	34.0	41.0
40	37.70920687800573	40.0	35.0	41.0	33.0	41.0
41	37.5575795393428	40.0	35.0	41.0	33.0	41.0
42	37.51327196110888	40.0	35.0	41.0	33.0	41.0
43	37.43068628628419	40.0	35.0	41.0	33.0	41.0
44	37.31666344662653	39.0	35.0	41.0	33.0	41.0
45	37.22522544632367	39.0	35.0	41.0	33.0	41.0
46	37.18348484927601	39.0	35.0	41.0	33.0	41.0
47	37.15995384028949	39.0	35.0	41.0	33.0	41.0
48	37.060130333300265	39.0	35.0	41.0	33.0	41.0
49	36.999391151329704	39.0	35.0	41.0	33.0	41.0
50	36.913762277490875	39.0	35.0	41.0	33.0	41.0
51	36.78669931961422	38.0	35.0	41.0	32.0	41.0
52	36.46886099347811	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	5.0
12	9.0
13	25.0
14	39.0
15	69.0
16	103.0
17	184.0
18	342.0
19	589.0
20	850.0
21	1271.0
22	1952.0
23	2925.0
24	4080.0
25	5240.0
26	6618.0
27	8378.0
28	10663.0
29	13574.0
30	17580.0
31	23195.0
32	30473.0
33	41389.0
34	87596.0
35	183913.0
36	110412.0
37	165206.0
38	280566.0
39	916338.0
40	1502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.733161365784376	14.364755703387308	45.48788829767791	12.414194633150402
2	25.319280033836527	13.34673566255372	48.97096220020991	12.36302210339984
3	25.926144463184496	14.294158499078371	44.960289072576224	14.819407965160906
4	23.863317128698913	14.204293270812338	47.03183662386624	14.900552976622508
5	23.67831276859051	16.978784286900357	44.1502488133717	15.19265413113744
6	24.324809800061615	20.05200799962404	43.08100402592046	12.542178174393893
7	85.62281668224469	2.7081755948806583	6.94030045585325	4.728707267021393
8	86.38711496587628	3.1574495193437384	7.10770773175151	3.3477277830284735
9	78.35965933715909	4.932457482415971	9.251627860831606	7.456255319593335
10	40.182811251690524	24.775389146202006	15.46501730989144	19.576782292216034
11	31.479512712196296	23.160634748236376	26.352129664924362	19.00772287464297
12	27.969808207447173	24.17828927100032	27.49343372896313	20.35846879258938
13	22.18000198424095	25.55498697189166	33.02648961667598	19.23852142719141
14	18.17016432648074	33.31723313264651	28.69604039496838	19.81656214590437
15	16.602248458297	24.43049673905665	36.42706086920197	22.54019393344438
16	20.14448407124469	23.030144797372447	34.02968006725533	22.795691064127535
17	19.944232385945305	23.253894072863414	28.33386420481544	28.46800933637584
18	21.352207990225004	23.76353069568532	30.704457753943682	24.179803560146
19	24.37932420930609	25.764950994470233	27.416779368071474	22.438945428152202
20	26.688458505866564	22.04100068404096	26.146604076048646	25.123936734043834
21	23.667295009634014	26.486170362750578	27.320230380817613	22.5263042467978
22	28.224156567054287	21.68843239743302	25.047177939417992	25.0402330960947
23	25.988961354296663	25.35118453962999	23.019022604681762	25.64083150139158
24	25.199964492530373	20.475591225477654	30.473398117059773	23.851046164932196
25	28.330365674720248	23.729119780271425	24.31906594468145	23.62144860032688
26	23.608133299218316	27.3952660188294	23.937151778767575	25.059448903184705
27	23.84269146619741	25.072398686223625	25.33854805779363	25.74636178978534
28	22.21707595987656	26.00013576385444	28.307494686933772	23.475293589335227
29	22.049094298440284	22.88942034055841	28.953104031664306	26.108381329337004
30	23.4335200956613	26.48517824227582	28.250317217467586	21.830984444595295
31	22.57094966816181	30.72487454897681	24.29368854727454	22.41048723558684
32	24.2444480416064	24.737375266958733	27.85352124443238	23.16465544700249
33	25.251241456015123	26.599637614942377	25.670542898767163	22.47857803027534
34	22.098178153507146	25.576656971735012	25.585324971672353	26.739839903085493
35	23.33597898793268	23.649175756752946	30.278890287140552	22.735954968173818
36	26.054597956231824	26.318710869985225	24.716801821324324	22.90988935245863
37	23.358641108250787	26.29672756893932	27.285453947334066	23.059177375475826
38	21.14689126881765	25.364917575675292	30.931548908928562	22.55664224657849
39	21.433979604091714	27.98134813507459	26.199551979280344	24.385120281553345
40	26.068069907941666	24.204606572014892	28.50513552887854	21.222187991164905
41	22.28610665817272	24.082471319885748	27.888141027314646	25.743280994626883
42	21.775582348610246	26.538073928640433	24.099024066754044	27.58731965599528
43	21.871818034661555	23.539468118991795	27.639014354416762	26.949699491929884
44	25.87424089729464	23.96101488702881	25.373794443081003	24.790949772595543
45	20.70492770574751	25.610284634142523	27.184988695048272	26.499798965061693
46	21.373564688865798	26.639218000198422	25.717746946618696	26.269470364317083
47	22.317123477225614	24.548715726153862	30.52812139377262	22.60603940284791
48	21.5310507600165	22.238276007916078	32.70551253465895	23.525160697408477
49	20.703517850336013	23.2815167955553	27.72183030562532	28.293135048483357
50	20.57109587538967	23.615756961813805	31.698927987718594	24.114219175077935
51	22.615072920854896	23.94482765823016	27.98437671336595	25.455722707548993
52	21.90466244406268	22.44672574134897	28.913941381345005	26.734670433243345
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	279.0
1	240.0
2	201.0
3	151.5
4	102.0
5	147.5
6	193.0
7	316.0
8	439.0
9	792.0
10	1145.0
11	1345.5
12	1546.0
13	1530.5
14	1477.5
15	1440.0
16	1667.0
17	1894.0
18	2403.5
19	2913.0
20	3342.0
21	3771.0
22	4326.5
23	4882.0
24	5888.5
25	6895.0
26	9621.5
27	12348.0
28	16502.5
29	20657.0
30	23146.0
31	25635.0
32	28939.0
33	32243.0
34	39622.5
35	47002.0
36	52746.5
37	58491.0
38	61106.5
39	76057.5
40	88393.0
41	105817.0
42	123241.0
43	139955.5
44	156670.0
45	179205.5
46	201741.0
47	199320.5
48	196900.0
49	170989.0
50	145078.0
51	129257.0
52	113436.0
53	105060.0
54	96684.0
55	91578.5
56	86473.0
57	85323.0
58	84173.0
59	81996.5
60	79820.0
61	74985.5
62	70151.0
63	63584.0
64	49360.0
65	41703.0
66	34273.0
67	26843.0
68	23848.5
69	20854.0
70	17509.0
71	14164.0
72	12506.5
73	10849.0
74	8120.0
75	5391.0
76	4821.0
77	4251.0
78	2945.0
79	1639.0
80	1232.5
81	826.0
82	844.0
83	862.0
84	603.5
85	345.0
86	242.0
87	139.0
88	95.5
89	40.5
90	29.0
91	26.0
92	23.0
93	21.5
94	20.0
95	12.0
96	4.0
97	4.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1915090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.761987451862627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.32233620030867	10.611338184193423
2	12.514940551955098	3.9452067188144597
3	5.5726369708318275	2.6350750202411106
4	3.1067217301228816	1.9587243570650323
5	1.9471595728085045	1.5345552376690919
6	1.304820329030198	1.233993699186555
7	0.9433169317386411	1.0407984748835812
8	0.6670654474495902	0.8411421769817253
9	0.49492146136435367	0.7020851277314211
>10	3.3719738072504093	10.949128393465669
>50	0.9653645151409204	11.205242338769148
>100	1.7721554782713027	48.904120738961346
>500	0.010615682385715215	1.082640202870141
>1k	0.003980880894643205	1.4322956018845157
>5k	0.0019904404473216025	1.9236537272827634
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCTT	8272	0.4319379245884006	Illumina Single End Adapter 2 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	6153	0.3212903832195876	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	6152	0.3212381663524952	Illumina Single End Adapter 2 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	5915	0.3088627688515944	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	5251	0.2741907691022354	Illumina Single End Adapter 2 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	5046	0.26348631134829176	Illumina Single End Adapter 2 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4688	0.2447926729292096	Illumina Single End Adapter 2 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4496	0.23476703444746724	Illumina Single End Adapter 2 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	3183	0.16620628795513528	Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	2822	0.1473559989347759	Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	2130	0.11122192690682943	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	2011	0.10500811972283287	Illumina Single End Adapter 2 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4501093943365586	0.0
2	0.0	0.0	0.0	0.9962978241231483	0.0
3	0.0	0.0	0.0	3.512889733641761	0.0
4	5.221686709240819E-5	0.0	0.0	4.059965850168922	0.0
5	5.221686709240819E-5	0.0	0.0	4.957939313557065	0.0
6	5.221686709240819E-5	0.0	0.0	7.748460907842452	0.0
7	1.0443373418481638E-4	0.0	0.0	8.076435050049867	0.0
8	1.0443373418481638E-4	0.0	0.0	8.727788250160566	0.0
9	1.0443373418481638E-4	0.0	0.0	9.143904464019968	0.0
10	1.0443373418481638E-4	0.0	0.0	10.53772929731762	0.0
11	1.0443373418481638E-4	0.0	0.0	11.733495553733768	0.0
12	1.0443373418481638E-4	0.0	0.0	12.254619887316	0.0
13	1.0443373418481638E-4	0.0	0.0	12.490431259105316	0.0
14	1.0443373418481638E-4	0.0	0.0	12.630059161710415	0.0
15	1.0443373418481638E-4	0.0	0.0	12.849056702295975	0.0
16	1.0443373418481638E-4	0.0	0.0	13.402451059741317	0.0
17	1.0443373418481638E-4	0.0	0.0	14.127430042452312	0.0
18	1.0443373418481638E-4	0.0	0.0	14.912406205452486	0.0
19	1.5665060127722456E-4	0.0	0.0	15.387631912860492	0.0
20	1.5665060127722456E-4	0.0	0.0	15.890793644162937	0.0
21	1.5665060127722456E-4	0.0	0.0	16.68532549384102	0.0
22	1.5665060127722456E-4	0.0	0.0	17.365815705789284	0.0
23	2.0886746836963277E-4	0.0	0.0	18.07481632717	0.0
24	3.133012025544491E-4	0.0	0.0	18.64246588933157	0.0
25	3.6551806964685735E-4	0.0	0.0	19.11831819914469	0.0
26	4.1773493673926554E-4	0.0	0.0	19.54597434063151	0.0
27	4.1773493673926554E-4	0.0	0.0	19.9484097353127	0.0
28	4.1773493673926554E-4	0.0	0.0	20.35862544319066	0.0
29	4.1773493673926554E-4	0.0	0.0	20.796620524361778	0.0
30	4.1773493673926554E-4	0.0	0.0	21.247565388571818	0.0
31	4.1773493673926554E-4	0.0	0.0	21.67172299996345	0.0
32	4.1773493673926554E-4	0.0	0.0	22.076664804265075	0.0
33	4.1773493673926554E-4	0.0	0.0	22.502545572270755	0.0
34	4.1773493673926554E-4	0.0	0.0	22.914327786161486	0.0
35	4.1773493673926554E-4	0.0	0.0	23.419317107812166	0.0
36	4.1773493673926554E-4	0.0	0.0	23.873708285250302	0.0
37	4.1773493673926554E-4	0.0	0.0	24.305541776104516	0.0
38	4.699518038316737E-4	0.0	0.0	24.710379146671958	0.0
39	6.266024051088982E-4	0.0	0.0	25.133074685785	0.0
40	6.266024051088982E-4	0.0	0.0	25.55973870679707	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCG	20	6.312512E-4	46.000004	11
ATCTAAC	20	6.312512E-4	46.000004	23
ACGTTAC	20	6.312512E-4	46.000004	23
ATTACGC	35	1.0200529E-7	46.000004	14
TCTAACG	20	6.312512E-4	46.000004	33
ATAAGCG	40	5.6152203E-9	46.000004	33
AACGACA	20	6.312512E-4	46.000004	36
GCGATGA	20	6.312512E-4	46.000004	41
TTACGAC	35	1.0200529E-7	46.000004	19
TGAATCG	35	1.0200529E-7	46.000004	36
ATACCGG	20	6.312512E-4	46.000004	24
GTAACGC	35	1.0200529E-7	46.000004	15
TACGTCC	40	5.6152203E-9	46.000004	22
GTACGCT	35	1.0200529E-7	46.000004	33
AACGTTG	25	3.4180146E-5	46.0	10
CGTGGTA	30	1.8620576E-6	46.0	11
GTCGACA	25	3.4180146E-5	46.0	37
TTTACGA	25	3.4180146E-5	46.0	26
CTATACG	25	3.4180146E-5	46.0	12
CGCATGT	30	1.8620576E-6	46.0	44
>>END_MODULE