##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545652_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2125307
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87535871288242	31.0	31.0	34.0	31.0	34.0
2	32.53862430227727	34.0	31.0	34.0	31.0	34.0
3	32.515126050024776	34.0	31.0	34.0	31.0	34.0
4	35.9973152114024	37.0	35.0	37.0	35.0	37.0
5	35.77574675093998	37.0	35.0	37.0	35.0	37.0
6	35.77962948411688	37.0	35.0	37.0	35.0	37.0
7	35.75737528742906	37.0	35.0	37.0	33.0	37.0
8	35.729222178254716	37.0	35.0	37.0	33.0	37.0
9	37.86832725813259	39.0	38.0	39.0	35.0	39.0
10	37.782185820683786	39.0	38.0	39.0	35.0	39.0
11	37.61297638411768	39.0	37.0	39.0	35.0	39.0
12	37.18091880373047	39.0	37.0	39.0	33.0	39.0
13	36.95139102256756	39.0	37.0	39.0	33.0	39.0
14	37.70857716085253	40.0	36.0	41.0	33.0	41.0
15	37.62779024395064	40.0	36.0	41.0	33.0	41.0
16	37.635494072150514	40.0	36.0	41.0	33.0	41.0
17	37.7337895184084	40.0	36.0	41.0	33.0	41.0
18	37.77229219119873	40.0	36.0	41.0	33.0	41.0
19	37.74135971885473	40.0	36.0	41.0	33.0	41.0
20	37.66641854565011	40.0	36.0	41.0	33.0	41.0
21	37.65498537387775	40.0	36.0	41.0	33.0	41.0
22	37.60909600354208	40.0	35.0	41.0	33.0	41.0
23	37.547653115526366	40.0	35.0	41.0	32.0	41.0
24	37.4888601976091	40.0	35.0	41.0	32.0	41.0
25	37.40395575792109	39.0	35.0	41.0	32.0	41.0
26	37.30512485960852	39.0	35.0	41.0	32.0	41.0
27	37.189514267821075	39.0	35.0	41.0	32.0	41.0
28	37.12403243390249	39.0	35.0	41.0	32.0	41.0
29	37.07384956620385	39.0	35.0	41.0	31.0	41.0
30	37.011754537109226	39.0	35.0	41.0	31.0	41.0
31	36.927802430425345	39.0	35.0	41.0	31.0	41.0
32	36.8484364847055	39.0	35.0	41.0	31.0	41.0
33	36.73038342225382	39.0	35.0	41.0	31.0	41.0
34	36.65789695324017	39.0	35.0	41.0	31.0	41.0
35	36.56627630737583	39.0	35.0	41.0	30.0	41.0
36	36.472910031350764	39.0	35.0	41.0	30.0	41.0
37	36.33992689056216	38.0	35.0	41.0	30.0	41.0
38	36.28011953096659	38.0	35.0	41.0	30.0	41.0
39	36.222443157623815	38.0	35.0	41.0	30.0	41.0
40	36.14049029152024	38.0	35.0	41.0	30.0	41.0
41	36.084623539093414	38.0	35.0	40.0	30.0	41.0
42	35.94715351711541	38.0	35.0	40.0	29.0	41.0
43	35.83197768604724	38.0	35.0	40.0	29.0	41.0
44	35.78110691773	38.0	35.0	40.0	29.0	41.0
45	35.75332740164127	38.0	35.0	40.0	29.0	41.0
46	35.68216874079839	38.0	35.0	40.0	29.0	41.0
47	35.593211710119995	37.0	34.0	40.0	29.0	41.0
48	35.527663532844905	37.0	34.0	40.0	29.0	41.0
49	35.49825742822096	37.0	34.0	40.0	29.0	41.0
50	35.37868458533285	37.0	34.0	40.0	28.0	41.0
51	35.22749748624552	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	14.0
11	21.0
12	30.0
13	37.0
14	49.0
15	62.0
16	105.0
17	300.0
18	592.0
19	1129.0
20	2015.0
21	3133.0
22	4855.0
23	7211.0
24	10355.0
25	14670.0
26	19992.0
27	24310.0
28	28805.0
29	34360.0
30	41823.0
31	51278.0
32	64683.0
33	83199.0
34	133722.0
35	243687.0
36	158621.0
37	215496.0
38	324278.0
39	656137.0
40	325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.6297377273024556	90.47511724188553	3.33161279758642	3.5635322332255996
2	3.6917019517650864	2.7795513777538963	90.14020092156098	3.388545748920038
3	29.02808864789887	15.929275158835877	41.539269385552295	13.503366807712958
4	27.495133644221752	15.920899898226468	42.772926452507804	13.811040005043978
5	25.444088783408702	14.440596111526476	45.03424681704808	15.081068288016745
6	23.52620115588007	15.171831645969263	46.32544851167385	14.976518686476826
7	22.98698493911703	18.083787424593247	42.993224037750785	15.936003598538939
8	25.592867289290446	19.73780729089962	42.63228794710599	12.037037472703943
9	80.47162127636149	4.8036824797546895	9.670602882313002	5.054093361570822
10	81.01126096135758	5.170688281740002	9.346179163763164	4.47187159313925
11	74.29571351338889	7.430502981451621	11.167421930102334	7.106361575057156
12	41.911074494178955	23.125129687146377	17.162744017687796	17.80105180098687
13	33.363132949733846	24.52055161913079	24.272305130505853	17.84401030062951
14	28.55573335993341	23.65799388041351	28.021316449811724	19.764956309841356
15	22.605393009104098	26.665465271605466	31.299478145980792	19.429663573309643
16	17.26188263624973	33.119685767750255	30.027473677920412	19.5909579180796
17	17.53440797023677	24.503236473601227	38.385560297876964	19.57679525828504
18	21.12513627443	21.3166380198249	37.03441432226027	20.523811383484833
19	20.79450168846195	21.992775631944	29.338914330964894	27.873808348629165
20	21.835057241142103	22.890763546160624	32.20137137834675	23.07280783435052
21	25.700192960358198	23.888313547172242	27.71839550709615	22.693097985373406
22	26.822054413785867	22.095537256499885	27.672002209563136	23.410406120151112
23	25.882613664755254	24.326979584596483	27.77099967204738	22.01940707860088
24	28.312427334027507	21.847102559771365	25.960531819638295	23.879938286562837
25	26.63196422916783	24.984061126227882	25.015962399785067	23.36801224481922
26	26.099429400081963	22.081562804808904	30.23469079996443	21.5843169951447
27	26.567832317872192	23.58732173751839	26.756322733609778	23.088523210999632
28	22.977292221782548	27.518989021350798	25.694593769276626	23.809124987590028
29	21.75775076259571	27.415098148173417	26.63370515412597	24.193445935104904
30	20.36345807923279	27.575498504451357	28.7674674764634	23.293575939852452
31	20.55580676109381	27.56641746345351	27.8500941275778	24.02768164787487
32	22.056248814877097	26.624624113128124	26.83207649530162	24.487050576693157
33	23.860035279609015	27.820639559367187	24.722169550093234	23.59715561093056
34	25.669467987448403	28.063710325143614	24.379583749547713	21.88723793786027
35	25.19664217922399	25.602371798521347	24.698831745249038	24.502154277005626
36	22.120098414017363	27.924059912285614	26.576819254818247	23.37902241887878
37	22.54427242746577	26.470058208061236	27.39867699113587	23.586992373337125
38	25.19301917323003	26.620295326745737	24.294937154961612	23.89174834506262
39	23.168605759073866	28.079378649766834	26.60575625074401	22.14625934041529
40	22.774874406379876	27.48002994390928	26.57371382110914	23.171381828601703
41	22.32976224140795	28.257235307652024	25.163047032734564	24.249955418205463
42	24.60675093057144	24.592447114699194	27.435471675386193	23.365330279343173
43	21.488989590680312	25.2240264582952	28.639203653872126	24.647780297152362
44	21.41652947080116	26.71345833801893	25.956720605540752	25.913291585639158
45	21.459205658288425	24.187893796049227	27.402441153207512	26.950459392454828
46	23.677567523186063	25.47711930558738	26.491655087947297	24.35365808327926
47	20.299561428066628	26.600392319791915	26.570184919166973	26.529861332974487
48	21.07300262973773	27.0015578925774	26.236162587334444	25.68927689035043
49	22.885587823312115	25.46272138566334	28.66367070733781	22.988020083686735
50	21.59523306515247	23.902382102915016	30.01312281002227	24.489262021910246
51	20.73968607829363	24.548218210357376	27.631584519318857	27.080511192030137
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	748.0
1	1673.0
2	2598.0
3	6042.5
4	9487.0
5	6697.5
6	3908.0
7	4073.0
8	4238.0
9	4437.5
10	4637.0
11	4954.0
12	5271.0
13	5205.5
14	5140.0
15	5184.0
16	5228.0
17	5464.5
18	5701.0
19	6515.5
20	7330.0
21	6450.0
22	5570.0
23	7765.5
24	9961.0
25	12195.5
26	19435.5
27	24441.0
28	27582.5
29	30724.0
30	34685.0
31	38646.0
32	44340.5
33	50035.0
34	55988.0
35	61941.0
36	67511.0
37	73081.0
38	89109.0
39	105137.0
40	123443.0
41	141749.0
42	158813.5
43	175878.0
44	193059.0
45	210240.0
46	198478.0
47	186716.0
48	169814.5
49	152913.0
50	143240.5
51	133568.0
52	120048.5
53	106529.0
54	110737.5
55	114946.0
56	113455.0
57	111964.0
58	106314.5
59	100665.0
60	86068.5
61	71472.0
62	61320.5
63	51169.0
64	43490.0
65	35811.0
66	28153.0
67	20495.0
68	19112.5
69	17730.0
70	14987.5
71	12245.0
72	9216.5
73	6188.0
74	4773.5
75	2530.5
76	1702.0
77	1243.0
78	784.0
79	672.5
80	561.0
81	407.0
82	253.0
83	154.0
84	55.0
85	41.5
86	28.0
87	22.0
88	16.0
89	10.5
90	5.0
91	8.0
92	11.0
93	6.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2125307.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.875575354837087
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.86650504067792	10.690556112213837
2	10.951062507947519	3.2580671110500905
3	4.875360780035617	2.1757138999631143
4	2.6287617259901896	1.564173725795147
5	1.669676265001009	1.2418697549102722
6	1.1198413603970745	0.9994970725229976
7	0.79591423304639	0.8287777504768258
8	0.5600876942377392	0.6665301360760354
9	0.3764767234681143	0.5040277082362887
>10	2.5212492865294722	7.800271261807463
>50	0.7419765091770046	8.162513624537016
>100	1.8698812547237205	57.59504317080012
>500	0.015577045474554676	1.5014821998922316
>1k	0.006993775519187814	2.3345775958010675
>5k	6.357977744716194E-4	0.6768988759175272
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	9019	0.42436222155199227	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5295	0.24914047711695297	No Hit
ATGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	3974	0.18698475090892752	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	3889	0.1829853287078055	No Hit
ATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	3832	0.180303363231759	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	3324	0.15640093407681807	No Hit
ATGCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	3116	0.14661411269054306	No Hit
ATGTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	2903	0.13659203117479027	No Hit
ATTGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	2845	0.1338630136728482	No Hit
ATATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	2397	0.11278370607164048	No Hit
ATAGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	2345	0.11033700072507172	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2276	0.10709041093827858	No Hit
ATGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	2263	0.10647873460163637	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.410405179110594E-5	0.0	0.0	0.1668935358515264	0.0
2	9.410405179110594E-5	0.0	0.0	0.7521736859663098	0.0
3	9.410405179110594E-5	0.0	0.0	1.0194762450789463	0.0
4	9.410405179110594E-5	0.0	0.0	1.3663908320068583	0.0
5	9.410405179110594E-5	0.0	0.0	2.681165591606295	0.0
6	1.4115607768665892E-4	0.0	0.0	2.9872860720827625	0.0
7	2.3526012947776485E-4	0.0	0.0	3.4593119958669503	0.0
8	2.3526012947776485E-4	0.0	0.0	4.8863528892531765	0.0
9	2.8231215537331784E-4	0.0	0.0	5.0651505876562775	0.0
10	2.8231215537331784E-4	0.0	0.0	5.409242053030456	0.0
11	2.8231215537331784E-4	0.0	0.0	5.6697220683882374	0.0
12	2.8231215537331784E-4	0.0	0.0	6.386042110622136	0.0
13	4.2346823305997673E-4	0.0	0.0	7.012445731369633	0.0
14	4.2346823305997673E-4	0.0	0.0	7.287605978806827	0.0
15	4.705202589555297E-4	0.0	0.0	7.440713271070956	0.0
16	5.175722848510827E-4	0.0	0.0	7.554861485893567	0.0
17	5.175722848510827E-4	0.0	0.0	7.713662073291059	0.0
18	5.175722848510827E-4	0.0	0.0	8.039497352617763	0.0
19	5.646243107466357E-4	0.0	0.0	8.439345468678171	0.0
20	5.646243107466357E-4	0.0	0.0	8.907089658105864	0.0
21	5.646243107466357E-4	0.0	0.0	9.200600195642323	0.0
22	6.587283625377416E-4	0.0	0.0	9.512272815174466	0.0
23	6.587283625377416E-4	0.0	0.0	9.968771570413121	0.0
24	7.057803884332946E-4	0.0	0.0	10.379818068636672	0.0
25	7.528324143288475E-4	0.0	0.0	10.799663295702691	0.0
26	7.528324143288475E-4	0.0	0.0	11.119758227870138	0.0
27	7.528324143288475E-4	0.0	0.0	11.414398014028091	0.0
28	7.528324143288475E-4	0.0	0.0	11.693651787718197	0.0
29	7.528324143288475E-4	0.0	0.0	11.947638623502392	0.0
30	7.998844402244005E-4	0.0	0.0	12.217952512272344	0.0
31	8.469364661199535E-4	0.0	0.0	12.513674495025896	0.0
32	8.469364661199535E-4	0.0	0.0	12.829393588785056	0.0
33	8.939884920155064E-4	0.0	0.0	13.132926207837269	0.0
34	8.939884920155064E-4	0.0	0.0	13.421402178602904	0.0
35	8.939884920155064E-4	0.0	0.0	13.712042542559734	0.0
36	8.939884920155064E-4	0.0	0.0	14.006070652381045	0.0
37	9.410405179110594E-4	0.0	0.0	14.352279458920522	0.0
38	9.410405179110594E-4	0.0	0.0	14.664046182504457	0.0
39	9.880925438066125E-4	0.0	0.0	14.977600883072421	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	30	2.1663745E-6	45.000004	44
AAATCGT	30	2.1663745E-6	45.000004	40
CTCCGAT	20	7.03464E-4	45.000004	22
CCGATCA	20	7.03464E-4	45.000004	24
GACACGT	20	7.03464E-4	45.000004	11
CCAATCG	40	6.8193913E-9	45.000004	18
ACCGAAC	30	2.1663745E-6	45.000004	34
CGTTCGA	20	7.03464E-4	45.000004	21
AATGCGT	30	2.1663745E-6	45.000004	32
CTACGAC	30	2.1663745E-6	45.000004	31
ACTACGT	20	7.03464E-4	45.000004	29
ACTACGA	30	2.1663745E-6	45.000004	44
ACGCTAA	20	7.03464E-4	45.000004	29
TACTACG	30	2.1663745E-6	45.000004	43
CGGTCTA	30	2.1663745E-6	45.000004	33
CAATCGT	40	6.8193913E-9	45.000004	19
CGATCAA	30	2.1663745E-6	45.000004	34
TCGACTA	20	7.03464E-4	45.000004	2
TCGACGA	20	7.03464E-4	45.000004	2
TGTCGAC	30	2.1663745E-6	45.000004	29
>>END_MODULE