FastQCFastQC Report
Thu 26 May 2016
SRR1545651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545651_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1702921
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT65690.3857489572328957Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT60890.35756209477715056No Hit
GTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC56860.33389687484034786No Hit
TGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC50050.2939067637312594No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC46420.2725904489991021No Hit
TTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC42390.24892522906229944No Hit
GGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC37810.22203026446910926No Hit
ATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC37360.21938774611388315No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC31470.1848001169754792No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC23650.13887902022466103No Hit
TCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC17780.10440883634648934No Hit
CTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC17590.10329310637428278No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA206.312314E-446.00000434
ACACGCG206.312314E-446.00000440
TCCGCAT206.312314E-446.00000434
CGTATCG206.312314E-446.0000041
GATCGAC206.312314E-446.00000435
CGAAATC405.6134013E-946.00000420
TTTACGA206.312314E-446.00000413
CGAAACA551.8189894E-1246.00000427
CAACGAC206.312314E-446.00000434
CGACGGA206.312314E-446.00000417
CTATCGA405.6134013E-946.00000430
GCGAAGT206.312314E-446.00000443
GACCGAA405.6134013E-946.00000444
TAACGTA206.312314E-446.00000437
CGCGACG405.6134013E-946.0000041
CCGTCGA405.6134013E-946.00000412
ACCTAAC206.312314E-446.00000441
CGAATTG206.312314E-446.00000417
CATACGT206.312314E-446.00000442
TCCCGTA206.312314E-446.00000441
Produced by FastQC (version 0.11.5)