##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545651_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1702921
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.543805026774585	33.0	31.0	34.0	31.0	34.0
2	32.95107406626614	34.0	31.0	34.0	31.0	34.0
3	33.03419771087443	34.0	33.0	34.0	31.0	34.0
4	36.41496933797868	37.0	37.0	37.0	35.0	37.0
5	36.38540719152562	37.0	37.0	37.0	35.0	37.0
6	36.39484215650638	37.0	37.0	37.0	35.0	37.0
7	36.462488864721266	37.0	37.0	37.0	35.0	37.0
8	36.48614527626355	37.0	37.0	37.0	35.0	37.0
9	38.279200855471274	39.0	39.0	39.0	37.0	39.0
10	38.06234757807321	39.0	39.0	39.0	35.0	39.0
11	37.901895037996475	39.0	38.0	39.0	35.0	39.0
12	37.54733836742867	39.0	37.0	39.0	35.0	39.0
13	37.49000805087259	39.0	37.0	39.0	35.0	39.0
14	38.81451987496778	41.0	38.0	41.0	35.0	41.0
15	38.858620570184996	41.0	38.0	41.0	35.0	41.0
16	38.91410464725023	41.0	38.0	41.0	35.0	41.0
17	38.87187602948111	41.0	38.0	41.0	35.0	41.0
18	38.82893393175608	41.0	38.0	41.0	35.0	41.0
19	38.807116712989036	40.0	38.0	41.0	35.0	41.0
20	38.75325103160981	40.0	38.0	41.0	35.0	41.0
21	38.70857720352265	40.0	38.0	41.0	35.0	41.0
22	38.80150165509733	40.0	38.0	41.0	35.0	41.0
23	38.77520859746283	40.0	38.0	41.0	35.0	41.0
24	38.730653389088516	40.0	38.0	41.0	35.0	41.0
25	38.623098194220404	40.0	37.0	41.0	35.0	41.0
26	38.51811035274097	40.0	37.0	41.0	35.0	41.0
27	38.4518882555327	40.0	37.0	41.0	35.0	41.0
28	38.427560057101886	40.0	37.0	41.0	35.0	41.0
29	38.3743262312227	40.0	36.0	41.0	35.0	41.0
30	38.34209983904127	40.0	36.0	41.0	35.0	41.0
31	38.26906650396583	40.0	36.0	41.0	34.0	41.0
32	38.20740832957019	40.0	36.0	41.0	34.0	41.0
33	38.13922783264755	40.0	36.0	41.0	34.0	41.0
34	38.06097405575479	40.0	36.0	41.0	34.0	41.0
35	37.982138337597576	40.0	35.0	41.0	34.0	41.0
36	37.9353786816887	40.0	35.0	41.0	34.0	41.0
37	37.85975802753034	40.0	35.0	41.0	34.0	41.0
38	37.75973988223764	40.0	35.0	41.0	34.0	41.0
39	37.696700551581664	40.0	35.0	41.0	34.0	41.0
40	37.54926446969648	40.0	35.0	41.0	33.0	41.0
41	37.405629503658716	39.0	35.0	41.0	33.0	41.0
42	37.38018322635049	39.0	35.0	41.0	33.0	41.0
43	37.301931211136626	39.0	35.0	41.0	33.0	41.0
44	37.18276361616305	39.0	35.0	41.0	33.0	41.0
45	37.102269570931355	39.0	35.0	41.0	33.0	41.0
46	37.07763777650285	39.0	35.0	41.0	33.0	41.0
47	37.070800700678426	39.0	35.0	41.0	33.0	41.0
48	36.95536551607503	39.0	35.0	41.0	33.0	41.0
49	36.90313173658672	39.0	35.0	41.0	33.0	41.0
50	36.8121574635582	38.0	35.0	41.0	33.0	41.0
51	36.7066804625699	38.0	35.0	40.0	33.0	41.0
52	36.38989477491909	38.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	6.0
12	11.0
13	30.0
14	26.0
15	34.0
16	78.0
17	153.0
18	283.0
19	459.0
20	759.0
21	1232.0
22	1841.0
23	2549.0
24	3533.0
25	4612.0
26	6063.0
27	7653.0
28	9760.0
29	12371.0
30	16052.0
31	21089.0
32	27810.0
33	39297.0
34	89582.0
35	184867.0
36	99002.0
37	143118.0
38	249808.0
39	779632.0
40	1201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.07903537509961	13.754660374732591	44.92827324344464	12.238031006723155
2	25.83114542600626	13.185168307866308	48.60865536334334	12.375030902784099
3	26.559188594186107	14.141466339307579	43.921356304843265	15.377988761663048
4	24.15479050408093	14.444533833336953	46.44325837781083	14.957417284771285
5	23.64631124990531	17.517371622054107	42.76475538207586	16.071561745964726
6	25.316030514627514	19.370833996409697	43.22801821106205	12.085117277900736
7	85.10929162304065	3.1729011504350466	6.67030355489186	5.047503671632448
8	85.77185905864101	3.3701504649951466	6.95005816476513	3.9079323115987177
9	77.99733516704534	5.106519914899164	9.506665312131332	7.389479605924174
10	42.90234250443796	22.37813732991724	15.327252409242705	19.392267756402088
11	34.038102765777154	23.191445757025722	24.408707156703098	18.361744320494022
12	29.10974731065035	23.807152533793406	26.7924348810074	20.29066527454885
13	22.972175456172074	25.41444964270216	32.34935736889732	19.264017532228447
14	17.014529740369635	34.010326961732225	29.51569685264319	19.459446445254947
15	17.270442962415757	24.298308612084764	37.48958407348315	20.94166435201633
16	20.810125660556185	22.253469186180684	35.52331552667446	21.413089626588665
17	20.771662337830115	22.435626784800938	27.563639182322607	29.229071695046333
18	21.697718214761576	23.31699474021402	30.294476373243384	24.690810671781016
19	25.16029809955952	24.875023562455333	27.273784280069364	22.69089405791578
20	27.279950156231557	21.586027772280687	25.773479803232213	25.360542268255543
21	25.71575545782805	25.063875540908825	27.154459895673376	22.06590910558975
22	28.88924383456426	21.436050174964077	24.78893618670508	24.885769803766586
23	26.906885287103748	25.262827811742294	23.131842287457843	24.698444613696115
24	26.989801640827732	20.70777211626376	28.76246167614352	23.539964566764986
25	28.46550133564622	23.192502764367813	24.43331193872176	23.908683961264206
26	23.154979003723604	27.037190803331452	24.503015700669614	25.30481449227533
27	22.14976502139559	27.294278477979894	24.667380342364677	25.888576158259834
28	21.334460024863162	25.6293157462971	27.514723231435866	25.521500997403873
29	20.659267223787833	27.456059323949848	25.669247134776068	26.21542631748625
30	21.928615596378222	27.819200068588035	24.697563774244372	25.55462056078937
31	23.232903933887712	28.686826928553938	22.189696409874564	25.890572727683786
32	25.653744360425414	29.604955250419717	21.7269033619293	23.014397027225574
33	24.715885234840606	25.481510886294785	23.05973089767523	26.74287298118938
34	20.64364700417694	29.006630372166413	26.37115873255424	23.978563891102407
35	21.848517928899813	26.7218502796078	26.337451942867578	25.092179848624802
36	24.527150701647347	25.672241988911992	23.296500542303487	26.50410676713717
37	23.398266860294754	27.5410309697279	26.185536498757134	22.875165671220216
38	21.68961449180555	26.90259853510527	27.540443743426735	23.867343229662445
39	22.293635465180124	28.881844783169626	24.461851136958206	24.362668614692048
40	25.41397986166123	24.0531416313499	27.250941176954186	23.281937330034687
41	21.422720137927715	24.34235058467187	28.477422029559797	25.75750724784062
42	21.562010216563188	26.456952495153914	24.94684133908737	27.034195949195528
43	21.389130793501284	23.030075969466584	27.31682796794449	28.26396526908764
44	26.26539927571508	24.43178515033874	23.947029838730042	25.355785735216134
45	20.08478373336168	25.67529556567803	25.728145932782553	28.511774768177737
46	20.78951401738542	26.570522061798524	25.049312328640028	27.59065159217603
47	23.256627876454633	24.729861220808246	28.6211750280841	23.39233587465302
48	21.18207479971179	22.60081354331763	31.024692278737533	25.19241937823305
49	20.37604797873771	23.12127221403694	26.5158512931604	29.98682851406495
50	20.05542241830361	24.56537913385295	30.333233309120033	25.045965138723403
51	21.90254274860666	24.73632071012102	27.00131127633049	26.359825264941826
52	21.04137537795353	22.86248158311513	28.179757017501107	27.916386021430235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	318.0
1	272.5
2	227.0
3	168.5
4	110.0
5	150.0
6	190.0
7	315.5
8	441.0
9	789.0
10	1137.0
11	1105.5
12	1074.0
13	1313.0
14	1438.0
15	1324.0
16	1356.5
17	1389.0
18	1817.0
19	2245.0
20	2211.0
21	2177.0
22	2760.5
23	3344.0
24	3854.5
25	4365.0
26	6963.0
27	9561.0
28	11739.0
29	13917.0
30	16758.5
31	19600.0
32	22670.0
33	25740.0
34	30547.0
35	35354.0
36	37470.5
37	39587.0
38	44026.0
39	62662.0
40	76859.0
41	91733.0
42	106607.0
43	117813.0
44	129019.0
45	149441.5
46	169864.0
47	173039.0
48	176214.0
49	155395.5
50	134577.0
51	123495.5
52	112414.0
53	105522.5
54	98631.0
55	93407.5
56	88184.0
57	87332.0
58	86480.0
59	85855.5
60	85231.0
61	76659.5
62	68088.0
63	59055.5
64	43506.5
65	36990.0
66	30011.0
67	23032.0
68	19457.5
69	15883.0
70	14315.0
71	12747.0
72	10890.5
73	9034.0
74	7149.5
75	5265.0
76	3910.0
77	2555.0
78	2100.0
79	1645.0
80	1277.0
81	909.0
82	584.5
83	260.0
84	204.5
85	149.0
86	108.0
87	67.0
88	51.0
89	26.5
90	18.0
91	13.0
92	8.0
93	6.0
94	4.0
95	4.5
96	5.0
97	6.0
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1702921.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.320666745167271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.1343781581413	10.438641016500867
2	11.976664529718033	3.6698097195695096
3	5.391294872256344	2.477946961883058
4	3.0749865974428667	1.8844337962111195
5	1.859811480215063	1.4246775948605617
6	1.2808263855687714	1.1773868527029763
7	0.9539448512998332	1.0230549812023026
8	0.6842851864675787	0.8386964240419535
9	0.5084132610710371	0.7010307127523776
>10	3.262475986436976	10.277404067168193
>50	0.9738837698692037	11.025274224518384
>100	1.8844535258740032	51.005817712209925
>500	0.008058139695246158	0.8359739790593778
>1k	0.00498837219229524	1.8471967929693887
>5k	0.0015348837514754586	1.3726551643500289
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	6569	0.3857489572328957	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	6089	0.35756209477715056	No Hit
GTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	5686	0.33389687484034786	No Hit
TGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	5005	0.2939067637312594	No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	4642	0.2725904489991021	No Hit
TTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	4239	0.24892522906229944	No Hit
GGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	3781	0.22203026446910926	No Hit
ATCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	3736	0.21938774611388315	No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	3147	0.1848001169754792	No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2365	0.13887902022466103	No Hit
TCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1778	0.10440883634648934	No Hit
CTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1759	0.10329310637428278	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.40160406736425236	0.0
2	0.0	0.0	0.0	0.9553584693594125	0.0
3	0.0	0.0	0.0	3.396810539067872	0.0
4	0.0	0.0	0.0	3.937822130327831	0.0
5	0.0	0.0	0.0	4.767044390197784	0.0
6	0.0	0.0	0.0	7.357534495140996	0.0
7	0.0	0.0	0.0	7.679451953437652	0.0
8	0.0	0.0	0.0	8.31530059233517	0.0
9	5.872263011613575E-5	0.0	0.0	8.724949660025333	0.0
10	5.872263011613575E-5	0.0	0.0	10.02853332597343	0.0
11	5.872263011613575E-5	0.0	0.0	11.123769100269477	0.0
12	5.872263011613575E-5	0.0	0.0	11.593726309088913	0.0
13	5.872263011613575E-5	0.0	0.0	11.835135041496347	0.0
14	5.872263011613575E-5	0.0	0.0	11.971136652845317	0.0
15	5.872263011613575E-5	0.0	0.0	12.200624691339176	0.0
16	5.872263011613575E-5	0.0	0.0	12.712274967541067	0.0
17	5.872263011613575E-5	0.0	0.0	13.376075578373865	0.0
18	5.872263011613575E-5	0.0	0.0	14.140702945116068	0.0
19	5.872263011613575E-5	0.0	0.0	14.618235373220484	0.0
20	5.872263011613575E-5	0.0	0.0	15.113032254579045	0.0
21	5.872263011613575E-5	0.0	0.0	15.854699072945838	0.0
22	5.872263011613575E-5	0.0	0.0	16.484440558311277	0.0
23	5.872263011613575E-5	0.0	0.0	17.144130585035946	0.0
24	5.872263011613575E-5	0.0	0.0	17.639103634284854	0.0
25	5.872263011613575E-5	0.0	0.0	18.075706389198324	0.0
26	5.872263011613575E-5	0.0	0.0	18.494927245597417	0.0
27	5.872263011613575E-5	0.0	0.0	18.861532625412455	0.0
28	5.872263011613575E-5	0.0	0.0	19.256207422423003	0.0
29	5.872263011613575E-5	0.0	0.0	19.680008643971153	0.0
30	5.872263011613575E-5	0.0	0.0	20.117785851486946	0.0
31	5.872263011613575E-5	0.0	0.0	20.54411214613009	0.0
32	5.872263011613575E-5	0.0	0.0	20.938140994209363	0.0
33	1.174452602322715E-4	0.0	0.0	21.342622470449303	0.0
34	1.174452602322715E-4	0.0	0.0	21.740938070527054	0.0
35	1.174452602322715E-4	0.0	0.0	22.185585825766434	0.0
36	1.174452602322715E-4	0.0	0.0	22.600989711207976	0.0
37	1.174452602322715E-4	0.0	0.0	23.019799509196258	0.0
38	1.174452602322715E-4	0.0	0.0	23.431680036830834	0.0
39	1.174452602322715E-4	0.0	0.0	23.858887170925723	0.0
40	1.174452602322715E-4	0.0	0.0	24.271472370121693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAA	20	6.312314E-4	46.000004	34
ACACGCG	20	6.312314E-4	46.000004	40
TCCGCAT	20	6.312314E-4	46.000004	34
CGTATCG	20	6.312314E-4	46.000004	1
GATCGAC	20	6.312314E-4	46.000004	35
CGAAATC	40	5.6134013E-9	46.000004	20
TTTACGA	20	6.312314E-4	46.000004	13
CGAAACA	55	1.8189894E-12	46.000004	27
CAACGAC	20	6.312314E-4	46.000004	34
CGACGGA	20	6.312314E-4	46.000004	17
CTATCGA	40	5.6134013E-9	46.000004	30
GCGAAGT	20	6.312314E-4	46.000004	43
GACCGAA	40	5.6134013E-9	46.000004	44
TAACGTA	20	6.312314E-4	46.000004	37
CGCGACG	40	5.6134013E-9	46.000004	1
CCGTCGA	40	5.6134013E-9	46.000004	12
ACCTAAC	20	6.312314E-4	46.000004	41
CGAATTG	20	6.312314E-4	46.000004	17
CATACGT	20	6.312314E-4	46.000004	42
TCCCGTA	20	6.312314E-4	46.000004	41
>>END_MODULE