FastQCFastQC Report
Thu 26 May 2016
SRR1545650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545650_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1052078
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC76110.7234254494438626Illumina PCR Primer Index 7 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG42400.40301194398133977RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT34400.3269719545508983RNA PCR Primer, Index 16 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30740.29218365938647134No Hit
ATGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT27140.2579656641427727RNA PCR Primer, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC25830.2455141158735379RNA PCR Primer, Index 16 (96% over 25bp)
TAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC24150.2295457180931452No Hit
ATTGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC23290.22137141922937273No Hit
ATATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC21340.20283667180570264RNA PCR Primer, Index 16 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC19540.18572767418385328No Hit
ATGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC18440.17527217563716757RNA PCR Primer, Index 16 (95% over 22bp)
ATTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC17750.16871372654879202No Hit
ATGTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT15980.15188987888730682No Hit
ATGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC14470.13753733088231102RNA PCR Primer, Index 16 (95% over 22bp)
ATAGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC13520.12850758213744606RNA PCR Primer, Index 16 (95% over 22bp)
ATCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT13150.12499073262628817No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT11720.11139858451559677No Hit
ATTCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC11440.1087371848855313RNA PCR Primer, Index 16 (95% over 22bp)
ATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC10660.10132328591606327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG302.165325E-645.00000415
TTCTACG302.165325E-645.00000425
TAGAGAC302.165325E-645.00000425
GTCGATT302.165325E-645.00000428
CACGGTT302.165325E-645.00000418
CGAAATG302.165325E-645.00000437
CACGGCT302.165325E-645.00000427
CAACGAC302.165325E-645.00000438
GCGAAGT302.165325E-645.0000043
TAGCGTA600.045.00000426
ACTCTAC302.165325E-645.00000425
GATCCGT302.165325E-645.00000429
ATTCGAT1300.045.0000041
TCCTATC302.165325E-645.0000048
ATGTCCA302.165325E-645.00000414
ATCACGG302.165325E-645.00000438
CCGTCCG302.165325E-645.00000413
GCCGCAA302.165325E-645.00000445
CCCCGTT302.165325E-645.00000417
GCCGAGT302.165325E-645.00000430
Produced by FastQC (version 0.11.5)