##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545650_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1052078
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96280979166944	31.0	31.0	34.0	31.0	34.0
2	32.63150165672127	34.0	31.0	34.0	31.0	34.0
3	32.552978011136055	34.0	31.0	34.0	31.0	34.0
4	35.99207283110188	37.0	35.0	37.0	35.0	37.0
5	35.74153627392646	37.0	35.0	37.0	35.0	37.0
6	35.74945013582643	37.0	35.0	37.0	35.0	37.0
7	35.71897901106192	37.0	35.0	37.0	33.0	37.0
8	35.69715933609485	37.0	35.0	37.0	33.0	37.0
9	37.91174513676743	39.0	38.0	39.0	35.0	39.0
10	37.85667507542217	39.0	38.0	39.0	35.0	39.0
11	37.68735968245701	39.0	38.0	39.0	35.0	39.0
12	37.18510129477092	39.0	37.0	39.0	33.0	39.0
13	36.90072504129922	39.0	37.0	39.0	33.0	39.0
14	37.27529612823384	39.0	35.0	41.0	33.0	41.0
15	37.02652274831334	39.0	35.0	41.0	32.0	41.0
16	37.10517185988111	39.0	35.0	41.0	32.0	41.0
17	37.28212166778509	39.0	35.0	41.0	32.0	41.0
18	37.399719412439005	39.0	35.0	41.0	33.0	41.0
19	37.37803755995278	39.0	35.0	41.0	33.0	41.0
20	37.35509344364201	39.0	35.0	41.0	33.0	41.0
21	37.33004967312309	39.0	35.0	41.0	33.0	41.0
22	37.235881750212435	39.0	35.0	41.0	32.0	41.0
23	37.14409387897095	39.0	35.0	41.0	32.0	41.0
24	37.10764981303667	39.0	35.0	41.0	32.0	41.0
25	37.03355359583605	39.0	35.0	41.0	32.0	41.0
26	36.957843429859764	39.0	35.0	41.0	32.0	41.0
27	36.89226749347482	38.0	35.0	41.0	32.0	41.0
28	36.73801847391543	38.0	35.0	41.0	31.0	41.0
29	36.64645301964303	38.0	35.0	41.0	31.0	41.0
30	36.57391086972639	38.0	35.0	41.0	31.0	41.0
31	36.499881187516515	38.0	35.0	41.0	31.0	41.0
32	36.39798570067999	38.0	35.0	41.0	31.0	41.0
33	36.23946988721369	38.0	35.0	41.0	30.0	41.0
34	36.13089808930516	38.0	35.0	40.0	30.0	41.0
35	36.08713137238874	38.0	35.0	40.0	30.0	41.0
36	35.954860761274354	38.0	35.0	40.0	30.0	41.0
37	35.86053980788497	38.0	35.0	40.0	30.0	41.0
38	35.78754522003121	38.0	35.0	40.0	30.0	41.0
39	35.694342054486455	37.0	35.0	40.0	30.0	41.0
40	35.62615699596418	37.0	35.0	40.0	30.0	41.0
41	35.59892042225006	37.0	35.0	40.0	29.0	41.0
42	35.4807086546815	37.0	34.0	40.0	29.0	41.0
43	35.3995654314604	37.0	34.0	40.0	29.0	41.0
44	35.32292852811293	37.0	34.0	40.0	29.0	41.0
45	35.25220753594315	37.0	34.0	40.0	28.0	41.0
46	35.163747364739116	36.0	34.0	40.0	28.0	41.0
47	35.062199760854234	36.0	34.0	40.0	28.0	41.0
48	35.0615705299417	36.0	34.0	40.0	28.0	41.0
49	35.02968981387312	36.0	34.0	40.0	28.0	41.0
50	34.91607751516523	36.0	34.0	40.0	28.0	41.0
51	34.8110244677676	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	4.0
12	10.0
13	7.0
14	12.0
15	22.0
16	61.0
17	119.0
18	281.0
19	452.0
20	865.0
21	1542.0
22	2406.0
23	3691.0
24	5536.0
25	8142.0
26	11128.0
27	13623.0
28	15539.0
29	18448.0
30	22511.0
31	27371.0
32	34597.0
33	45365.0
34	73934.0
35	171625.0
36	74129.0
37	96464.0
38	140899.0
39	283132.0
40	155.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	1.3856387073962197	92.2683489246995	2.738865369297714	3.6071469986065674
2	3.4511699702873737	2.189571495649562	91.71183125205545	2.6474272820076075
3	24.820593149937555	15.7321985632244	46.10865354089716	13.33855474594089
4	26.740317733095836	14.643305914580479	45.61600945937469	13.000366892949003
5	23.700714205600725	13.320305148477585	48.36314417752296	14.61583646839873
6	21.97432129556934	14.111025988567388	49.37780278648541	14.53684992937786
7	22.450806879337843	17.097211423487614	46.243244322189035	14.208737374985505
8	24.695317267350898	18.163482175275977	44.71607618446541	12.425124372907712
9	81.42656723170715	3.632905545026129	10.252471774906423	4.688055448360292
10	82.3291619062465	4.08220683257325	9.777697090900105	3.8109341702801505
11	75.9061590490439	6.555787688745511	10.566707031227722	6.971346230982873
12	45.457846281359366	21.556101353701912	16.430150616209062	16.555901748729656
13	36.87663842414726	20.39497071509907	26.114033370149365	16.61435749060431
14	30.843625662736034	24.393248409338472	26.86350251597315	17.899623411952344
15	19.06892834941896	33.485444995523146	31.38503038748078	16.060596267577118
16	13.53806466820901	40.210516710738176	29.859192949572176	16.39222567148063
17	13.372012341290285	23.503580533002307	45.16794382165581	17.9564633040516
18	16.22969019407306	21.03085512671114	42.524413589106516	20.21504109010929
19	17.025923933396573	20.590678637895667	28.344001110183846	34.03939631852391
20	20.16970224641139	22.647940551936262	35.33682863818082	21.845528563471532
21	25.28434203547646	24.363212613513447	27.670762053764076	22.68168329724602
22	25.92431359652041	23.728468801742835	26.66152129404854	23.685696307688215
23	21.220765000313666	28.25750562220672	29.338223971986864	21.18350540549275
24	26.79192987592175	21.590604498905975	25.007651523936435	26.60981410123584
25	21.963770747035866	28.0691165483928	23.19609382574296	26.77101887882837
26	22.16470641910581	23.15845403097489	33.808519900615735	20.86831964930357
27	24.61671092827718	23.44930699054633	30.769296573067777	21.164685508108715
28	19.442474797496004	30.7197755299512	26.487199618279256	23.35055005427354
29	19.082235347569288	30.623584943321692	25.77318411752741	24.520995591581613
30	19.243915375095764	26.167261362750672	32.811160389248705	21.77766287290486
31	17.322860092122447	23.829126737751384	33.425563503846675	25.422449666279494
32	18.323736453000635	30.280929740950768	28.079286896979124	23.31604690906948
33	25.426917015658535	26.5926100536272	23.23896136978437	24.741511560929894
34	24.99339402591823	26.22048935535198	26.76626637948897	22.019850239240817
35	25.24518144091978	25.236626942108852	24.147639243478146	25.370552373493222
36	19.68228591416226	25.24461114099905	25.610648640119837	29.46245430471885
37	18.82151323380966	27.1073057320845	30.14510330983064	23.926077724275196
38	26.63709344744401	25.76491476867685	24.331560967913028	23.266430815966118
39	22.4002402863666	28.323090113090476	26.0436963799262	23.232973220616724
40	20.991694532154458	28.509102937234694	26.270770798362857	24.22843173224799
41	18.643294508582066	30.516083408264404	24.5990316307346	26.24159045241893
42	24.44020310281177	24.07331015380989	25.56664049623697	25.919846247141372
43	19.9285604299301	22.66723569925424	27.617248911202402	29.78695495961326
44	19.72021085889069	26.06090042753484	25.4319546649583	28.78693404861617
45	19.46994424367775	23.459762489093013	28.92722782911533	28.143065438113908
46	22.79298683177483	24.76118690819502	29.10753765405227	23.338288605977883
47	17.36135533677161	30.30260113793844	26.618368600046765	25.717674925243184
48	20.484888002600567	28.871813686817898	25.375685072779774	25.26761323780176
49	20.909951543516737	25.641159685878804	28.962206224253332	24.486682546351126
50	20.38622611631457	22.86512977174696	32.82351688753115	23.925127224407316
51	19.886358235796205	22.254338556646942	30.478158463535976	27.38114474402088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1283.0
1	1228.0
2	1173.0
3	3070.0
4	4967.0
5	3353.0
6	1739.0
7	1992.0
8	2245.0
9	2504.5
10	2764.0
11	2929.5
12	3095.0
13	3029.5
14	2964.0
15	3470.0
16	3976.0
17	3745.5
18	3515.0
19	3703.0
20	3891.0
21	3845.0
22	3799.0
23	4157.5
24	4516.0
25	5421.5
26	7251.5
27	8176.0
28	8987.0
29	9798.0
30	12183.5
31	14569.0
32	17956.0
33	21343.0
34	25138.5
35	28934.0
36	34313.5
37	39693.0
38	49748.5
39	59804.0
40	71058.0
41	82312.0
42	90621.5
43	98931.0
44	99270.5
45	99610.0
46	104386.5
47	109163.0
48	110245.5
49	111328.0
50	103823.5
51	96319.0
52	78432.5
53	60546.0
54	51420.5
55	42295.0
56	39280.0
57	36265.0
58	33267.5
59	30270.0
60	26137.0
61	22004.0
62	17445.0
63	12886.0
64	11715.0
65	10544.0
66	7967.5
67	5391.0
68	4226.5
69	3062.0
70	2051.5
71	1041.0
72	843.5
73	646.0
74	533.0
75	359.5
76	299.0
77	225.0
78	151.0
79	80.0
80	9.0
81	8.5
82	8.0
83	5.0
84	2.0
85	2.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1052078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.477120337861344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69532766924925	11.648554213307587
2	11.75250366213083	3.872948342241716
3	5.264789378995265	2.6024570445359783
4	2.873698904910927	1.8940113068399083
5	1.7596431777087462	1.4496926195401874
6	1.1432924554238304	1.1302900421632458
7	0.7701531258796082	0.8882934013588865
8	0.6213264733305554	0.8190136856133247
9	0.46094808398542164	0.683558734440097
>10	2.570976785969646	8.208090091651817
>50	0.5553898755873865	6.744644075811923
>100	1.5007097263267497	53.602637931453046
>500	0.020248589214123452	2.146439079840815
>1k	0.01041356016726349	3.583848809596984
>5k	5.785311204035272E-4	0.7255206216044963
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	7611	0.7234254494438626	Illumina PCR Primer Index 7 (95% over 22bp)
ATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTG	4240	0.40301194398133977	RNA PCR Primer, Index 16 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	3440	0.3269719545508983	RNA PCR Primer, Index 16 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3074	0.29218365938647134	No Hit
ATGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	2714	0.2579656641427727	RNA PCR Primer, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2583	0.2455141158735379	RNA PCR Primer, Index 16 (96% over 25bp)
TAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	2415	0.2295457180931452	No Hit
ATTGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	2329	0.22137141922937273	No Hit
ATATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	2134	0.20283667180570264	RNA PCR Primer, Index 16 (95% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1954	0.18572767418385328	No Hit
ATGTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1844	0.17527217563716757	RNA PCR Primer, Index 16 (95% over 22bp)
ATTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1775	0.16871372654879202	No Hit
ATGTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1598	0.15188987888730682	No Hit
ATGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1447	0.13753733088231102	RNA PCR Primer, Index 16 (95% over 22bp)
ATAGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1352	0.12850758213744606	RNA PCR Primer, Index 16 (95% over 22bp)
ATCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	1315	0.12499073262628817	No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT	1172	0.11139858451559677	No Hit
ATTCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1144	0.1087371848855313	RNA PCR Primer, Index 16 (95% over 22bp)
ATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	1066	0.10132328591606327	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.36774839888297256	0.0
2	0.0	0.0	0.0	1.4804035442239074	0.0
3	0.0	0.0	0.0	2.0715194120588016	0.0
4	0.0	0.0	0.0	2.6721402785725012	0.0
5	0.0	0.0	0.0	4.777877685875001	0.0
6	0.0	0.0	0.0	5.217293774796166	0.0
7	0.0	0.0	0.0	5.9233250766578145	0.0
8	0.0	0.0	0.0	8.130670919836742	0.0
9	0.0	0.0	0.0	8.410307980967191	0.0
10	9.504998678805184E-5	0.0	0.0	8.9782316520258	0.0
11	9.504998678805184E-5	0.0	0.0	9.368031647843601	0.0
12	9.504998678805184E-5	0.0	0.0	10.409589403067073	0.0
13	9.504998678805184E-5	0.0	0.0	11.387653767116126	0.0
14	9.504998678805184E-5	0.0	0.0	11.849216502958907	0.0
15	1.9009997357610368E-4	0.0	0.0	12.068021572545002	0.0
16	1.9009997357610368E-4	0.0	0.0	12.20422820361228	0.0
17	1.9009997357610368E-4	0.0	0.0	12.39347272730729	0.0
18	1.9009997357610368E-4	0.0	0.0	12.846005714405205	0.0
19	1.9009997357610368E-4	0.0	0.0	13.435505732464703	0.0
20	2.851499603641555E-4	0.0	0.0	14.176230279503992	0.0
21	2.851499603641555E-4	0.0	0.0	14.570212474740465	0.0
22	2.851499603641555E-4	0.0	0.0	15.017612762551826	0.0
23	2.851499603641555E-4	0.0	0.0	15.60825338045278	0.0
24	2.851499603641555E-4	0.0	0.0	16.08977661352105	0.0
25	0.0025663496432773996	0.0	0.0	16.665779533456647	0.0
26	0.0026613996300654513	0.0	0.0	17.07725092626212	0.0
27	0.0026613996300654513	0.0	0.0	17.436539876320957	0.0
28	0.0027564496168535034	0.0	0.0	17.768739580145198	0.0
29	0.0027564496168535034	0.0	0.0	18.066436138765376	0.0
30	0.0027564496168535034	0.0	0.0	18.384568444544986	0.0
31	0.0027564496168535034	0.0	0.0	18.717243398303168	0.0
32	0.0027564496168535034	0.0	0.0	19.13270689055374	0.0
33	0.0027564496168535034	0.0	0.0	19.51138603791734	0.0
34	0.0027564496168535034	0.0	0.0	19.85147489064499	0.0
35	0.0027564496168535034	0.0	0.0	20.192704343214096	0.0
36	0.0027564496168535034	0.0	0.0	20.519296097817843	0.0
37	0.0027564496168535034	0.0	0.0	20.969547885232846	0.0
38	0.0027564496168535034	0.0	0.0	21.352124082054754	0.0
39	0.0027564496168535034	0.0	0.0	21.69630008421429	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	30	2.165325E-6	45.000004	15
TTCTACG	30	2.165325E-6	45.000004	25
TAGAGAC	30	2.165325E-6	45.000004	25
GTCGATT	30	2.165325E-6	45.000004	28
CACGGTT	30	2.165325E-6	45.000004	18
CGAAATG	30	2.165325E-6	45.000004	37
CACGGCT	30	2.165325E-6	45.000004	27
CAACGAC	30	2.165325E-6	45.000004	38
GCGAAGT	30	2.165325E-6	45.000004	3
TAGCGTA	60	0.0	45.000004	26
ACTCTAC	30	2.165325E-6	45.000004	25
GATCCGT	30	2.165325E-6	45.000004	29
ATTCGAT	130	0.0	45.000004	1
TCCTATC	30	2.165325E-6	45.000004	8
ATGTCCA	30	2.165325E-6	45.000004	14
ATCACGG	30	2.165325E-6	45.000004	38
CCGTCCG	30	2.165325E-6	45.000004	13
GCCGCAA	30	2.165325E-6	45.000004	45
CCCCGTT	30	2.165325E-6	45.000004	17
GCCGAGT	30	2.165325E-6	45.000004	30
>>END_MODULE