FastQCFastQC Report
Thu 26 May 2016
SRR1545649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545649_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences901498
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT76000.8430412491209077RNA PCR Primer, Index 16 (96% over 27bp)
TTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC47970.5321143252674992Illumina PCR Primer Index 7 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC42740.47409977615036303Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT42720.4738779231900681Illumina PCR Primer Index 7 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC36270.40233034349493846RNA PCR Primer, Index 16 (96% over 25bp)
ATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC33890.3759298412198363RNA PCR Primer, Index 16 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC29950.3322248080417261Illumina PCR Primer Index 7 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC24210.2685530084370681RNA PCR Primer, Index 16 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC20220.22429334285822045RNA PCR Primer, Index 16 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC19150.21242420948243923RNA PCR Primer, Index 16 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT18770.20820900323683467RNA PCR Primer, Index 16 (96% over 26bp)
CTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC16040.1779260741565705RNA PCR Primer, Index 16 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC14480.1606215432535624Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT13150.1458683213939465Illumina PCR Primer Index 7 (95% over 23bp)
GTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT10870.1205770839203193No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT10310.11436520103205997No Hit
AACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC9570.10615664150114587RNA PCR Primer, Index 16 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC9530.10571293558055593RNA PCR Primer, Index 16 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA351.0192343E-746.00000440
GATACTA351.0192343E-746.00000425
ACACGTG351.0192343E-746.00000442
CAAACCG351.0192343E-746.00000444
TGCCCCG351.0192343E-746.00000418
AGATCCG700.046.00000424
ACGCCTC700.046.00000423
GCGACTA351.0192343E-746.00000437
CACGGTA351.0192343E-746.00000429
TAGACGT351.0192343E-746.00000427
CCCTAGA351.0192343E-746.00000428
TAGCGTA351.0192343E-746.00000424
GACGTCC351.0192343E-746.00000427
CATTGAG351.0192343E-746.00000420
CCGTGTA351.0192343E-746.00000429
TAGTTCA351.0192343E-746.00000414
ATCCGAA351.0192343E-746.00000433
AGACGGG700.046.0000043
AACATCG351.0192343E-746.00000414
TGCGACG351.0192343E-746.00000415
Produced by FastQC (version 0.11.5)