##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545649_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	901498
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.555682874504434	33.0	31.0	34.0	31.0	34.0
2	32.95656673669825	34.0	31.0	34.0	31.0	34.0
3	33.00773268493108	34.0	33.0	34.0	31.0	34.0
4	36.39472744254563	37.0	37.0	37.0	35.0	37.0
5	36.35941510685548	37.0	37.0	37.0	35.0	37.0
6	36.37855103394572	37.0	37.0	37.0	35.0	37.0
7	36.47450133000849	37.0	37.0	37.0	35.0	37.0
8	36.525892458996026	37.0	37.0	37.0	35.0	37.0
9	38.33586652438497	39.0	39.0	39.0	37.0	39.0
10	38.08052264120386	39.0	39.0	39.0	35.0	39.0
11	37.88380673057511	39.0	38.0	39.0	35.0	39.0
12	37.306944663216115	39.0	35.0	39.0	35.0	39.0
13	37.167571087234805	39.0	35.0	39.0	35.0	39.0
14	38.418136257651156	40.0	36.0	41.0	35.0	41.0
15	38.50389241018838	40.0	36.0	41.0	35.0	41.0
16	38.573649636493926	40.0	36.0	41.0	35.0	41.0
17	38.5603950313811	40.0	36.0	41.0	35.0	41.0
18	38.5870184958813	40.0	36.0	41.0	35.0	41.0
19	38.56344994664436	40.0	36.0	41.0	35.0	41.0
20	38.46548633496691	40.0	36.0	41.0	35.0	41.0
21	38.41863431754702	40.0	35.0	41.0	35.0	41.0
22	38.49771158671456	40.0	35.0	41.0	35.0	41.0
23	38.47093837146616	40.0	35.0	41.0	35.0	41.0
24	38.44503814761652	40.0	35.0	41.0	35.0	41.0
25	38.40191547845919	40.0	35.0	41.0	35.0	41.0
26	38.25155907167847	40.0	35.0	41.0	35.0	41.0
27	38.14851946426947	40.0	35.0	41.0	35.0	41.0
28	38.13205575608598	40.0	35.0	41.0	35.0	41.0
29	38.0373056845384	40.0	35.0	41.0	35.0	41.0
30	37.99862783944057	40.0	36.0	41.0	34.0	41.0
31	37.89187552274104	40.0	35.0	41.0	34.0	41.0
32	37.80995742641692	40.0	35.0	41.0	34.0	41.0
33	37.82037785996197	40.0	35.0	41.0	34.0	41.0
34	37.69006475887911	40.0	35.0	41.0	34.0	41.0
35	37.682833461638296	40.0	35.0	41.0	34.0	41.0
36	37.61863032419373	40.0	35.0	41.0	34.0	41.0
37	37.50217304974609	40.0	35.0	41.0	34.0	41.0
38	37.41431262188047	39.0	35.0	41.0	33.0	41.0
39	37.3701893958722	39.0	35.0	41.0	33.0	41.0
40	37.29845656895523	39.0	35.0	41.0	33.0	41.0
41	37.19879245433712	39.0	35.0	41.0	33.0	41.0
42	37.181140723551245	39.0	35.0	41.0	33.0	41.0
43	37.05070005701621	39.0	35.0	41.0	33.0	41.0
44	36.90691826271384	38.0	35.0	41.0	33.0	41.0
45	36.81166458494639	38.0	35.0	40.0	33.0	41.0
46	36.87058762193593	38.0	35.0	41.0	33.0	41.0
47	36.87422822901437	38.0	35.0	41.0	33.0	41.0
48	36.78551033945721	38.0	35.0	41.0	33.0	41.0
49	36.79245433711445	38.0	35.0	41.0	33.0	41.0
50	36.688284388872745	38.0	35.0	40.0	33.0	41.0
51	36.57559750548531	38.0	35.0	40.0	33.0	41.0
52	36.248036046668986	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	5.0
13	13.0
14	6.0
15	11.0
16	29.0
17	55.0
18	81.0
19	148.0
20	277.0
21	481.0
22	828.0
23	1254.0
24	1964.0
25	2755.0
26	3517.0
27	4305.0
28	5261.0
29	6680.0
30	8850.0
31	11661.0
32	15225.0
33	21590.0
34	50680.0
35	147280.0
36	51380.0
37	64173.0
38	116601.0
39	385851.0
40	534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.505323361782278	13.874795063327927	48.66255942886174	12.957322146028055
2	24.4600653578821	12.677454636615943	50.82950821854292	12.032971786959038
3	24.59938901694735	13.327705663240517	46.64081340169363	15.432091918118509
4	22.48368826109431	13.69587065085003	49.74475816918063	14.07568291887503
5	23.07803234172455	16.76919970981633	45.60420544471535	14.54856250374377
6	23.797834271401598	17.912851720136928	45.311137684165686	12.978176324295784
7	83.13495981133624	2.598230944494608	8.172175645425725	6.094633598743425
8	85.04156415211126	2.8630124526066614	8.304732789201973	3.790690606080102
9	77.0986735411504	4.1624052410543335	10.425979869062383	8.312941348732886
10	45.455453034837575	20.47114913177844	14.953222303321803	19.120175530062188
11	35.981444218400924	18.40958049823738	28.26107212661592	17.347903156745772
12	30.330294687287157	24.47204541773803	26.376320302429956	18.821339592544852
13	19.47547304597459	31.759027751586803	33.351155521143696	15.414343681294913
14	13.361316386725205	40.59909173398055	29.95591781678939	16.08367406250485
15	13.183834018489227	23.251854136115664	43.71135598747862	19.85295585791649
16	15.541687280504227	22.5880700789131	40.98722348801661	20.883019152566064
17	16.393380795076638	21.913193373695783	26.925184526199725	34.76824130502785
18	19.20969319954121	23.61602577043987	33.285265191936084	23.889015838082837
19	23.304987920106313	25.7450377039106	28.266729377103445	22.683244998879644
20	25.576318527606272	22.321846526559128	25.65762763755438	26.444207308280216
21	20.264714952223965	29.830903673663165	29.675717527936836	20.228663846176033
22	26.22778974551247	21.330274720520734	24.85895698049247	27.58297855347433
23	22.090121109531026	28.487916778517537	21.89122993062658	27.53073218132486
24	21.715189606632514	21.203152974271713	32.843001315588054	24.238656103507715
25	26.128843325220906	23.70032989535196	28.128514982839675	22.04231179658746
26	18.99915474022128	31.23700773601272	24.732168013683893	25.031669510082104
27	19.161994813077786	30.46617962546783	24.65196816853726	25.719857392917124
28	21.488455881211053	24.70044304036171	31.88803524799833	21.923065830428907
29	17.520393833375117	21.683464633310333	33.04056137673073	27.75558015658382
30	18.66038526985085	30.953590579235897	26.295676751362734	24.090347399550527
31	25.359900964838523	26.389409627087357	21.620680245546854	26.630009162527262
32	23.999609538789883	27.910655375830007	24.714419776860293	23.37531530851982
33	24.31841224273376	23.80937062533694	22.938486829699013	28.933730302230288
34	17.199927232229022	25.439657104064565	24.157125140599316	33.20329052310709
35	18.330600844372366	27.10677117420116	28.853530457083654	25.709097524342816
36	25.715642186671516	23.023012807571398	24.062948558954098	27.198396446802985
37	22.88657323698999	26.38375237659984	24.70077581980215	26.02889856660802
38	18.925499557403345	26.598173262724934	28.07260803684534	26.403719143026382
39	18.54158300961289	29.973111421212252	24.01458461360979	27.47072095556507
40	24.362339128872165	23.28646319792168	26.13450057570843	26.216697097497722
41	19.950793013406575	21.90487388768472	27.234225699890622	30.910107399018077
42	20.197937211175176	26.090129983649433	23.518077688469635	30.19385511670575
43	19.927831232016043	21.97719795274088	29.004723249524677	29.090247565718393
44	25.983529636227704	22.88235803074438	25.761343896492285	25.37276843653563
45	17.573749470326057	27.801725572325175	26.21791728877934	28.40660766856943
46	20.539479843549294	26.612149999223515	24.818357888758488	28.030012268468706
47	21.351905384149493	24.055516484784214	29.144823393950958	25.447754737115336
48	19.3248348859343	20.947356511051606	34.35969907864466	25.368109524369437
49	19.775640101253693	20.129939278844766	29.123969215683232	30.970451404218313
50	18.82544387231031	24.009149216082566	31.006835289706686	26.15857162190044
51	21.067933594971926	24.70876252637277	26.46528333950824	27.758020539147065
52	18.212242290055	22.19195161830642	29.755917373083467	29.83988871855512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	741.0
1	566.5
2	392.0
3	306.5
4	221.0
5	241.0
6	261.0
7	367.5
8	474.0
9	653.0
10	832.0
11	935.0
12	1038.0
13	993.0
14	1192.5
15	1437.0
16	1438.0
17	1439.0
18	1648.5
19	1858.0
20	1951.0
21	2044.0
22	2197.0
23	2350.0
24	2424.0
25	2498.0
26	3626.0
27	4754.0
28	4858.5
29	4963.0
30	5142.0
31	5321.0
32	8289.0
33	11257.0
34	13623.5
35	15990.0
36	17551.5
37	19113.0
38	24506.5
39	37717.5
40	45535.0
41	52511.5
42	59488.0
43	65326.5
44	71165.0
45	74438.5
46	77712.0
47	80709.0
48	83706.0
49	100741.5
50	117777.0
51	112467.5
52	107158.0
53	87485.0
54	67812.0
55	54968.0
56	42124.0
57	37333.5
58	32543.0
59	29723.5
60	26904.0
61	23491.5
62	20079.0
63	18243.0
64	13302.5
65	10198.0
66	8177.5
67	6157.0
68	4853.0
69	3549.0
70	3061.5
71	2574.0
72	1924.0
73	1274.0
74	979.5
75	685.0
76	501.0
77	317.0
78	246.0
79	175.0
80	211.5
81	248.0
82	146.5
83	45.0
84	32.5
85	20.0
86	13.0
87	6.0
88	4.0
89	2.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	901498.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.268340612375034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.3837819374903	10.636856350843464
2	12.540701446996202	4.080328053156774
3	5.996215362351752	2.926454216996823
4	3.5893286911683613	2.335696868707887
5	2.2770612799068104	1.852200424838732
6	1.5753471639799048	1.537697054781847
7	1.1292156291445272	1.2859325135818376
8	0.8632333953999625	1.1234699923474885
9	0.6363418983599428	0.9317004073600393
>10	3.682809033698879	11.542808619160045
>50	0.7220393887544779	8.575081638517611
>100	1.5793330811734654	46.668811918866176
>500	0.013662050875202987	1.4272355077366314
>1k	0.010246538156402241	4.231142624119326
>5k	6.831025437601493E-4	0.8445838089853148
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCTT	7600	0.8430412491209077	RNA PCR Primer, Index 16 (96% over 27bp)
TTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	4797	0.5321143252674992	Illumina PCR Primer Index 7 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	4274	0.47409977615036303	Illumina PCR Primer Index 7 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	4272	0.4738779231900681	Illumina PCR Primer Index 7 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3627	0.40233034349493846	RNA PCR Primer, Index 16 (96% over 25bp)
ATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3389	0.3759298412198363	RNA PCR Primer, Index 16 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2995	0.3322248080417261	Illumina PCR Primer Index 7 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2421	0.2685530084370681	RNA PCR Primer, Index 16 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2022	0.22429334285822045	RNA PCR Primer, Index 16 (96% over 25bp)
AGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1915	0.21242420948243923	RNA PCR Primer, Index 16 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	1877	0.20820900323683467	RNA PCR Primer, Index 16 (96% over 26bp)
CTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1604	0.1779260741565705	RNA PCR Primer, Index 16 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1448	0.1606215432535624	Illumina PCR Primer Index 7 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	1315	0.1458683213939465	Illumina PCR Primer Index 7 (95% over 23bp)
GTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	1087	0.1205770839203193	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	1031	0.11436520103205997	No Hit
AACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	957	0.10615664150114587	RNA PCR Primer, Index 16 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	953	0.10571293558055593	RNA PCR Primer, Index 16 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8823092230931183	0.0
2	0.0	0.0	0.0	1.7925719191834035	0.0
3	0.0	0.0	0.0	5.576496009974509	0.0
4	0.0	0.0	0.0	6.360524371656953	0.0
5	0.0	0.0	0.0	7.5554244158056925	0.0
6	0.0	0.0	0.0	11.377839995207976	0.0
7	0.0	0.0	0.0	11.881668068037866	0.0
8	0.0	0.0	0.0	12.862036299581364	0.0
9	0.0	0.0	0.0	13.449613864922606	0.0
10	0.0	0.0	0.0	15.302862568746686	0.0
11	0.0	0.0	0.0	16.97097497720461	0.0
12	0.0	0.0	0.0	17.740582896467878	0.0
13	0.0	0.0	0.0	18.087782779329515	0.0
14	0.0	0.0	0.0	18.284677281591307	0.0
15	0.0	0.0	0.0	18.570423894451235	0.0
16	0.0	0.0	0.0	19.2652673660951	0.0
17	0.0	0.0	0.0	20.23476480258414	0.0
18	0.0	0.0	0.0	21.43632043554173	0.0
19	1.1092648014748785E-4	0.0	0.0	22.06028188637135	0.0
20	1.1092648014748785E-4	0.0	0.0	22.75978427018141	0.0
21	1.1092648014748785E-4	0.0	0.0	23.70343583679609	0.0
22	1.1092648014748785E-4	0.0	0.0	24.447530665625436	0.0
23	0.001442044241917342	0.0	0.0	25.388519996716575	0.0
24	0.001442044241917342	0.0	0.0	26.00959735906236	0.0
25	0.001442044241917342	0.0	0.0	26.538827595846026	0.0
26	0.001442044241917342	0.0	0.0	27.021912416888334	0.0
27	0.001442044241917342	0.0	0.0	27.449423071376753	0.0
28	0.001442044241917342	0.0	0.0	27.90599646366381	0.0
29	0.001442044241917342	0.0	0.0	28.365564870914856	0.0
30	0.001442044241917342	0.0	0.0	28.94204978824135	0.0
31	0.001442044241917342	0.0	0.0	29.4719455839059	0.0
32	0.001442044241917342	0.0	0.0	29.947265551337885	0.0
33	0.001442044241917342	0.0	0.0	30.424027563011787	0.0
34	0.001442044241917342	0.0	0.0	30.85397860006345	0.0
35	0.001442044241917342	0.0	0.0	31.434789650115697	0.0
36	0.001442044241917342	0.0	0.0	31.914657603233728	0.0
37	0.001442044241917342	0.0	0.0	32.34505234620598	0.0
38	0.001442044241917342	0.0	0.0	32.79729960576729	0.0
39	0.001442044241917342	0.0	0.0	33.26130507222423	0.0
40	0.001442044241917342	0.0	0.0	33.75980867400704	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	35	1.0192343E-7	46.000004	40
GATACTA	35	1.0192343E-7	46.000004	25
ACACGTG	35	1.0192343E-7	46.000004	42
CAAACCG	35	1.0192343E-7	46.000004	44
TGCCCCG	35	1.0192343E-7	46.000004	18
AGATCCG	70	0.0	46.000004	24
ACGCCTC	70	0.0	46.000004	23
GCGACTA	35	1.0192343E-7	46.000004	37
CACGGTA	35	1.0192343E-7	46.000004	29
TAGACGT	35	1.0192343E-7	46.000004	27
CCCTAGA	35	1.0192343E-7	46.000004	28
TAGCGTA	35	1.0192343E-7	46.000004	24
GACGTCC	35	1.0192343E-7	46.000004	27
CATTGAG	35	1.0192343E-7	46.000004	20
CCGTGTA	35	1.0192343E-7	46.000004	29
TAGTTCA	35	1.0192343E-7	46.000004	14
ATCCGAA	35	1.0192343E-7	46.000004	33
AGACGGG	70	0.0	46.000004	3
AACATCG	35	1.0192343E-7	46.000004	14
TGCGACG	35	1.0192343E-7	46.000004	15
>>END_MODULE