FastQCFastQC Report
Thu 26 May 2016
SRR1545648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545648_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2008270
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.2413520094409616No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC45900.22855492538353905No Hit
ATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG37340.18593117459305772No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT26080.12986301642707404Illumina Single End Adapter 1 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT23890.11895810822249997No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC22850.11377952167786205No Hit
ATGCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC20230.10073346711348574No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCG302.1663145E-645.00000417
TCGATAG302.1663145E-645.0000048
CATTACG600.045.00000426
CGTACGA302.1663145E-645.00000437
TACTCGA302.1663145E-645.00000441
AATCCGT253.8918515E-545.042
ACGCATC207.034548E-445.012
GACGTAA207.034548E-445.021
CCCAACG253.8918515E-545.034
TACGATC351.2125383E-745.013
ACTAACG207.034548E-445.038
AGTTACG207.034548E-445.03
CTCGAAT406.8193913E-945.043
TTACGCC253.8918515E-545.01
CGTCGAA253.8918515E-545.033
CGCAATA253.8918515E-545.033
GCCGTAT406.8193913E-945.024
ATACCCG351.2125383E-745.022
TAGTCGA253.8918515E-545.024
GCGTAAG253.8918515E-545.03