##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545648_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2008270
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.924259686197573	31.0	31.0	34.0	31.0	34.0
2	32.58680057960334	34.0	31.0	34.0	31.0	34.0
3	32.57792826661753	34.0	31.0	34.0	31.0	34.0
4	36.046736245624345	37.0	35.0	37.0	35.0	37.0
5	35.84791536994528	37.0	35.0	37.0	35.0	37.0
6	35.85173358163992	37.0	35.0	37.0	35.0	37.0
7	35.85560258331798	37.0	35.0	37.0	35.0	37.0
8	35.826192693213564	37.0	35.0	37.0	35.0	37.0
9	37.95482679121831	39.0	38.0	39.0	35.0	39.0
10	37.8456104009919	39.0	38.0	39.0	35.0	39.0
11	37.70489027869758	39.0	38.0	39.0	35.0	39.0
12	37.30407116572971	39.0	37.0	39.0	34.0	39.0
13	37.078050262165945	39.0	37.0	39.0	33.0	39.0
14	37.89879846833344	40.0	36.0	41.0	33.0	41.0
15	37.868071524247235	40.0	36.0	41.0	33.0	41.0
16	37.89179592385486	40.0	36.0	41.0	33.0	41.0
17	37.91744835106833	40.0	36.0	41.0	33.0	41.0
18	37.9776404567115	40.0	36.0	41.0	33.0	41.0
19	37.94317148590578	40.0	36.0	41.0	33.0	41.0
20	37.8886967389843	40.0	36.0	41.0	33.0	41.0
21	37.87204708530228	40.0	36.0	41.0	33.0	41.0
22	37.80571935048574	40.0	36.0	41.0	33.0	41.0
23	37.70995184910395	40.0	35.0	41.0	33.0	41.0
24	37.67140175374825	40.0	35.0	41.0	33.0	41.0
25	37.591166028472266	40.0	35.0	41.0	33.0	41.0
26	37.52804901731341	40.0	35.0	41.0	33.0	41.0
27	37.45180179955882	40.0	35.0	41.0	32.0	41.0
28	37.36596921728652	39.0	35.0	41.0	32.0	41.0
29	37.31447165968719	39.0	35.0	41.0	32.0	41.0
30	37.22561109810932	39.0	35.0	41.0	32.0	41.0
31	37.12442998202433	39.0	35.0	41.0	32.0	41.0
32	37.011204170753935	39.0	35.0	41.0	31.0	41.0
33	36.92504742888158	39.0	35.0	41.0	31.0	41.0
34	36.7941820571935	39.0	35.0	41.0	31.0	41.0
35	36.72613891558406	39.0	35.0	41.0	31.0	41.0
36	36.61866631478835	39.0	35.0	41.0	31.0	41.0
37	36.41831974784267	39.0	35.0	41.0	30.0	41.0
38	36.41071270297321	38.0	35.0	41.0	30.0	41.0
39	36.35720445955972	38.0	35.0	41.0	30.0	41.0
40	36.30578657252262	38.0	35.0	41.0	30.0	41.0
41	36.205164146255235	38.0	35.0	41.0	30.0	41.0
42	36.0437092622008	38.0	35.0	40.0	30.0	41.0
43	35.9598604769279	38.0	35.0	40.0	30.0	41.0
44	35.8959577148491	38.0	35.0	40.0	30.0	41.0
45	35.873311357536586	38.0	35.0	40.0	30.0	41.0
46	35.808095027063096	38.0	35.0	40.0	29.0	41.0
47	35.707526876366224	37.0	35.0	40.0	29.0	41.0
48	35.633570187275616	37.0	35.0	40.0	29.0	41.0
49	35.5813247222734	37.0	34.0	40.0	29.0	41.0
50	35.46413281082723	37.0	34.0	40.0	29.0	41.0
51	35.276057502228284	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	15.0
11	24.0
12	26.0
13	43.0
14	49.0
15	87.0
16	139.0
17	287.0
18	483.0
19	860.0
20	1525.0
21	2476.0
22	3900.0
23	5788.0
24	8652.0
25	12552.0
26	16810.0
27	21108.0
28	24460.0
29	29348.0
30	36345.0
31	45381.0
32	57592.0
33	75094.0
34	124183.0
35	221577.0
36	151137.0
37	213312.0
38	327665.0
39	627045.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.3745811071220504	90.69144089191195	3.2700782265332853	3.6638997744327204
2	3.6513516608822516	2.874464090983782	90.47025549353424	3.00392875459973
3	28.954274076692876	15.893281281899347	41.48849507287367	13.663949568534111
4	27.18892380008664	15.712130341039801	42.834180662958666	14.264765195914892
5	25.786522728517582	14.400752886813029	45.71576531044133	14.096959074228065
6	23.985121522504443	15.237044819670661	45.25307852031848	15.52475513750641
7	23.6047941760819	17.72326430211077	42.68405144726556	15.987890074541768
8	26.829111623437086	20.23343474731983	41.634491378151345	11.302962251091737
9	82.10768472366763	4.549139308957461	9.091855178835514	4.251320788539389
10	81.98982208567574	5.4090834399757	8.784824749660155	3.8162697246884134
11	75.79463916704428	7.461048564187086	10.31290613313947	6.431406135629174
12	42.28589781254512	24.093573075333495	15.931821916375785	17.688707195745593
13	33.685809179044654	24.752149860327545	23.25523958431884	18.30680137630896
14	29.592335691913934	23.54538981312274	27.046960817021613	19.815313677941713
15	22.733745960453525	25.314972588347185	31.024264665607713	20.92701678559158
16	19.245768746234322	31.049062128100307	29.80485691664965	19.90031220901572
17	18.43492159918736	24.459310750048548	37.733023945983355	19.37274370478073
18	22.22166342175106	21.51867029831646	35.58286485382941	20.676801426103065
19	22.843292983513173	21.2482385336633	29.168737271382835	26.73973121144069
20	22.974201676069452	23.541854431923994	30.703540858549893	22.780403033456658
21	27.289009943882046	24.220299063372952	27.153121841186696	21.337569151558306
22	29.63107550279594	21.559501461456875	26.70373007613518	22.105692959612007
23	27.049052169280024	23.709361789002475	26.981581161895562	22.26000487982194
24	28.197602911959045	22.42178591524048	25.034781179821437	24.34582999297903
25	28.324229311795722	24.617257639660007	24.43745113953801	22.62106190900626
26	26.594929964596393	21.183755172362282	28.443287008221006	23.77802785482032
27	27.659527852330612	22.114954662470684	26.014231154177477	24.211286331021228
28	24.871107968550046	26.29038924049057	25.003560278249438	23.834942512709944
29	24.760515269361193	25.173258575789113	27.013648563191207	23.052577591658494
30	23.94448953576959	24.901581958601184	28.56906690833404	22.584861597295184
31	24.807720077479622	24.773362147520007	26.77369078858918	23.64522698641119
32	24.838094479327978	24.803885931672536	26.90982786179149	23.448191727207995
33	26.338689518839598	25.574200680187424	23.663551215722986	24.423558585249992
34	28.83217894008276	25.4349265785975	25.043146588855087	20.68974789246466
35	28.340412394747716	25.235451408426158	23.962714176878606	22.461422019947516
36	25.906576306970674	24.90128319399284	26.32210808307648	22.870032415960008
37	24.78366952650789	24.02998600785751	27.639759594078484	23.546584871556114
38	27.04073655434777	26.990344923740334	23.79545579030708	22.173462731604815
39	27.066330722462617	27.787996633918745	24.521702759091156	20.62396988452748
40	25.49218979519686	26.39988646944883	25.43029572716816	22.67762800818615
41	25.13651052896274	26.13582834977369	24.62741563634372	24.100245484919856
42	25.689225054400055	23.281132517042032	28.28748126496935	22.742161163588563
43	22.95204330095057	24.88131575933515	27.820064035214386	24.34657690449989
44	23.966100175773178	26.05167631842332	24.97562578736923	25.006597718434275
45	23.446399139557926	24.66276944833115	26.08703013041075	25.803801281700167
46	25.65611197697521	24.433816170136485	26.36517998077948	23.54489187210883
47	23.08295199350685	24.86338988283448	26.242985255966577	25.81067286769209
48	22.420242298097367	26.373844154421466	25.507327202019646	25.698586345461514
49	24.464638718897362	24.243652496925215	28.100056267334573	23.191652516842858
50	24.314509503204253	22.487364746772098	29.36721655952636	23.830909190497295
51	22.694309032152052	24.25869031554522	27.11637379436032	25.930626857942407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	771.0
1	1716.5
2	2662.0
3	5849.5
4	9037.0
5	6400.5
6	3764.0
7	3680.0
8	3596.0
9	3886.0
10	4176.0
11	4182.5
12	4189.0
13	4136.5
14	4084.0
15	4243.5
16	4403.0
17	4483.5
18	4564.0
19	4953.5
20	5343.0
21	6845.0
22	8347.0
23	9859.0
24	11371.0
25	12769.5
26	17954.5
27	21741.0
28	24152.5
29	26564.0
30	30626.5
31	34689.0
32	38034.5
33	41380.0
34	45629.0
35	49878.0
36	53980.0
37	58082.0
38	69491.0
39	80900.0
40	95523.0
41	110146.0
42	118500.5
43	126855.0
44	131884.0
45	136913.0
46	146656.5
47	156400.0
48	163987.5
49	171575.0
50	161365.5
51	151156.0
52	144268.5
53	137381.0
54	132630.0
55	127879.0
56	128718.5
57	129558.0
58	120456.5
59	111355.0
60	100433.5
61	89512.0
62	74961.0
63	60410.0
64	46994.0
65	33578.0
66	30193.0
67	26808.0
68	22749.0
69	18690.0
70	14624.5
71	10559.0
72	8846.0
73	7133.0
74	6174.0
75	3420.0
76	1625.0
77	1398.5
78	1172.0
79	766.5
80	361.0
81	260.0
82	159.0
83	100.0
84	41.0
85	42.5
86	44.0
87	37.0
88	30.0
89	17.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2008270.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.952268335519959
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31837127849215	11.695943325577863
2	10.776081984073173	3.4380590283099512
3	4.374201826833521	2.093353238861098
4	2.1921379263997336	1.398782897215954
5	1.3019930635868224	1.0384871360661347
6	0.8238117764215428	0.7884999909262694
7	0.5879997945017756	0.6565951352186042
8	0.4258890497000299	0.5435117121579579
9	0.3240626172354528	0.4652580444885754
>10	2.690369199050898	10.03842552136998
>50	1.3008390298647003	15.471729723116248
>100	1.8685847967533253	49.37844852101969
>500	0.010020900535601679	1.0958135264777318
>1k	0.005636756551275944	1.8970921991939436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4847	0.2413520094409616	No Hit
ACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	4590	0.22855492538353905	No Hit
ATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	3734	0.18593117459305772	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	2608	0.12986301642707404	Illumina Single End Adapter 1 (95% over 21bp)
ATGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	2389	0.11895810822249997	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	2285	0.11377952167786205	No Hit
ATGCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	2023	0.10073346711348574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14485104094568957	0.0
2	0.0	0.0	0.0	0.5428552933619484	0.0
3	0.0	0.0	0.0	0.8202084381084217	0.0
4	0.0	0.0	0.0	1.1042339924412554	0.0
5	0.0	0.0	0.0	2.044396420799992	0.0
6	0.0	0.0	0.0	2.2552744401898153	0.0
7	0.0	0.0	0.0	2.626240495550897	0.0
8	0.0	0.0	0.0	3.7082165246704877	0.0
9	0.0	0.0	0.0	3.848536302389619	0.0
10	0.0	0.0	0.0	4.126885329163907	0.0
11	0.0	0.0	0.0	4.349962903394464	0.0
12	0.0	0.0	0.0	4.946097885244514	0.0
13	9.95882027814985E-5	0.0	0.0	5.4795420934436105	0.0
14	1.4938230417224776E-4	0.0	0.0	5.7075492837118516	0.0
15	1.4938230417224776E-4	0.0	0.0	5.839204887788992	0.0
16	1.4938230417224776E-4	0.0	0.0	5.948204175733343	0.0
17	1.4938230417224776E-4	0.0	0.0	6.0803577208243915	0.0
18	1.4938230417224776E-4	0.0	0.0	6.3481006040024495	0.0
19	1.99176405562997E-4	0.0	0.0	6.6776379670064285	0.0
20	1.99176405562997E-4	0.0	0.0	7.080771011865934	0.0
21	1.99176405562997E-4	0.0	0.0	7.347518013016178	0.0
22	1.99176405562997E-4	0.0	0.0	7.632290478869873	0.0
23	2.987646083444955E-4	0.0	0.0	8.035622700134942	0.0
24	2.987646083444955E-4	0.0	0.0	8.399418404895757	0.0
25	2.987646083444955E-4	0.0	0.0	8.780741633346114	0.0
26	3.4855870973524476E-4	0.0	0.0	9.065713275605372	0.0
27	4.481469125167433E-4	0.0	0.0	9.327381278413759	0.0
28	4.481469125167433E-4	0.0	0.0	9.57864231403148	0.0
29	4.979410139074926E-4	0.0	0.0	9.81391944310277	0.0
30	4.979410139074926E-4	0.0	0.0	10.053030717981148	0.0
31	5.477351152982418E-4	0.0	0.0	10.318632454799404	0.0
32	5.97529216688991E-4	0.0	0.0	10.593944041388857	0.0
33	6.473233180797403E-4	0.0	0.0	10.8693054220797	0.0
34	6.473233180797403E-4	0.0	0.0	11.164883207935189	0.0
35	6.473233180797403E-4	0.0	0.0	11.44143964705941	0.0
36	6.473233180797403E-4	0.0	0.0	11.722328173004627	0.0
37	6.473233180797403E-4	0.0	0.0	12.026171779690978	0.0
38	6.971174194704895E-4	0.0	0.0	12.329866004073157	0.0
39	6.971174194704895E-4	0.0	0.0	12.640282432143088	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAAGCG	30	2.1663145E-6	45.000004	17
TCGATAG	30	2.1663145E-6	45.000004	8
CATTACG	60	0.0	45.000004	26
CGTACGA	30	2.1663145E-6	45.000004	37
TACTCGA	30	2.1663145E-6	45.000004	41
AATCCGT	25	3.8918515E-5	45.0	42
ACGCATC	20	7.034548E-4	45.0	12
GACGTAA	20	7.034548E-4	45.0	21
CCCAACG	25	3.8918515E-5	45.0	34
TACGATC	35	1.2125383E-7	45.0	13
ACTAACG	20	7.034548E-4	45.0	38
AGTTACG	20	7.034548E-4	45.0	3
CTCGAAT	40	6.8193913E-9	45.0	43
TTACGCC	25	3.8918515E-5	45.0	1
CGTCGAA	25	3.8918515E-5	45.0	33
CGCAATA	25	3.8918515E-5	45.0	33
GCCGTAT	40	6.8193913E-9	45.0	24
ATACCCG	35	1.2125383E-7	45.0	22
TAGTCGA	25	3.8918515E-5	45.0	24
GCGTAAG	25	3.8918515E-5	45.0	3
>>END_MODULE