FastQCFastQC Report
Thu 26 May 2016
SRR1545647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545647_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1661019
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT72440.43611782887492556Illumina Single End Adapter 1 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC41190.2479803060651323No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT37780.22745073957612766Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC35440.21336300186813034No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC32670.19668649184627027No Hit
TTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC27620.16628346816020767No Hit
ATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC24700.14870389802886058No Hit
GGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC24040.14473043354711776No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT21730.13082330786101784Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC19830.11938454647418242No Hit
TCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC19590.1179396502990032No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA351.01994374E-746.00000412
TCTATCG351.01994374E-746.0000041
CGAGTTG600.046.01
GTCGACG206.312271E-446.012
CCGATAA206.312271E-446.046
AACCCGT206.312271E-446.043
TAGCGAT301.8619066E-646.014
ATTAACG253.417817E-546.01
CTAAGCG206.312271E-446.015
TCGATTG301.8619066E-646.01
TTATACG253.417817E-546.039
TACGCTA301.8619066E-646.021
TCAATCG301.8619066E-646.041
TACGCGA206.312271E-446.039
CTACGTA206.312271E-446.023
TTGACGA206.312271E-446.028
TCTAACG253.417817E-546.01
TCATGCG453.110472E-1046.01
TGTTACG453.110472E-1046.01
CATACCG301.8619066E-646.028
Produced by FastQC (version 0.11.5)