##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545647_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1661019
Sequences flagged as poor quality	0
Sequence length	52
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.596176202680404	33.0	31.0	34.0	31.0	34.0
2	33.008854805393554	34.0	33.0	34.0	31.0	34.0
3	33.08875756388097	34.0	33.0	34.0	31.0	34.0
4	36.45124649386913	37.0	37.0	37.0	35.0	37.0
5	36.441999158347976	37.0	37.0	37.0	35.0	37.0
6	36.449501781737595	37.0	37.0	37.0	35.0	37.0
7	36.51166603151439	37.0	37.0	37.0	35.0	37.0
8	36.53312876011653	37.0	37.0	37.0	35.0	37.0
9	38.343201974209805	39.0	39.0	39.0	37.0	39.0
10	38.12210396148389	39.0	39.0	39.0	35.0	39.0
11	37.96155131277848	39.0	38.0	39.0	35.0	39.0
12	37.63833706899199	39.0	37.0	39.0	35.0	39.0
13	37.60378598920301	39.0	37.0	39.0	35.0	39.0
14	38.972138789502104	41.0	38.0	41.0	35.0	41.0
15	38.96207930192249	41.0	38.0	41.0	35.0	41.0
16	39.026648099750815	41.0	38.0	41.0	35.0	41.0
17	38.98418862156303	41.0	38.0	41.0	35.0	41.0
18	38.957429746438784	41.0	38.0	41.0	35.0	41.0
19	38.93438064224431	41.0	38.0	41.0	35.0	41.0
20	38.86951865090044	40.0	38.0	41.0	35.0	41.0
21	38.77917290530692	40.0	38.0	41.0	35.0	41.0
22	38.876380703652394	41.0	38.0	41.0	35.0	41.0
23	38.863765555962935	41.0	38.0	41.0	35.0	41.0
24	38.844184804628966	41.0	38.0	41.0	35.0	41.0
25	38.759009379182295	41.0	38.0	41.0	35.0	41.0
26	38.65411473318487	40.0	38.0	41.0	35.0	41.0
27	38.57283751721082	40.0	37.0	41.0	35.0	41.0
28	38.53331117825865	40.0	37.0	41.0	35.0	41.0
29	38.50209178823361	40.0	37.0	41.0	35.0	41.0
30	38.400973137573985	40.0	37.0	41.0	35.0	41.0
31	38.34501170666922	40.0	36.0	41.0	35.0	41.0
32	38.16839783289655	40.0	36.0	41.0	34.0	41.0
33	38.15057202837535	40.0	36.0	41.0	34.0	41.0
34	38.02754513945957	40.0	36.0	41.0	34.0	41.0
35	37.83007539347834	40.0	35.0	41.0	34.0	41.0
36	37.86574566576301	40.0	35.0	41.0	34.0	41.0
37	37.790393126147265	40.0	35.0	41.0	34.0	41.0
38	37.74526119207547	40.0	35.0	41.0	34.0	41.0
39	37.59002154701421	40.0	35.0	41.0	33.0	41.0
40	37.43628640009536	39.0	35.0	41.0	33.0	41.0
41	37.33753256284245	39.0	35.0	41.0	33.0	41.0
42	37.291882272267806	39.0	35.0	41.0	33.0	41.0
43	37.232675243329545	39.0	35.0	41.0	33.0	41.0
44	37.11779215048112	39.0	35.0	41.0	33.0	41.0
45	37.012975769693185	39.0	35.0	41.0	33.0	41.0
46	36.98366364261938	39.0	35.0	41.0	33.0	41.0
47	36.93598086475832	39.0	35.0	41.0	33.0	41.0
48	36.82314049387755	38.0	35.0	41.0	33.0	41.0
49	36.721610649848074	38.0	35.0	40.0	32.0	41.0
50	36.606781740606216	38.0	35.0	40.0	32.0	41.0
51	36.47601081023155	38.0	35.0	40.0	32.0	41.0
52	36.12141281948009	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	2.0
10	3.0
11	6.0
12	8.0
13	15.0
14	26.0
15	48.0
16	86.0
17	156.0
18	219.0
19	354.0
20	707.0
21	1056.0
22	1675.0
23	2511.0
24	3268.0
25	4340.0
26	5643.0
27	7113.0
28	9019.0
29	11542.0
30	14973.0
31	19558.0
32	26018.0
33	37771.0
34	87636.0
35	170572.0
36	99103.0
37	150539.0
38	266400.0
39	739665.0
40	981.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.368971095454054	13.33013048014502	45.67912829413751	12.62177013026341
2	25.374604384417037	12.706055740482197	49.3225544078665	12.59678546723427
3	26.770494497654752	14.069796913822177	44.98052099343837	14.179187595084706
4	24.59315636967428	14.6519696644048	45.349752170203956	15.405121795716967
5	23.767458409566657	17.179875726888135	43.14688754312865	15.905778320416564
6	26.539732537677175	19.741797053495475	42.341779353517325	11.376691055310022
7	86.85258868200785	2.992018754752354	6.191380110642925	3.9640124525968696
8	87.17486073308012	3.492976299488446	6.244359637066163	3.0878033303652757
9	80.00113183533722	5.399577006644717	8.353607032791317	6.245684125226743
10	43.72291948496676	23.56523314904887	13.664864760728204	19.046982605256172
11	34.551260400994806	23.571735181837173	23.194015240042408	18.68298917712561
12	30.543299023069576	23.257289651713798	25.699164187766666	20.50024713744996
13	23.152715291035204	24.122662052631547	31.68464659344655	21.039976062886698
14	19.360404667255462	31.691570054285954	28.7758899807889	20.172135297669683
15	18.26174173805357	23.99159792874133	37.0197451082739	20.726915224931204
16	22.428822307270416	21.815764900943336	34.436210543046165	21.31920224874008
17	23.167766292859984	21.286752288805847	27.545560887623804	27.999920530710366
18	23.257289651713798	23.494613848486985	28.908218388832395	24.339878110966822
19	27.291620384836058	24.941436551899766	26.321372603203212	21.44557046006096
20	30.335836013916754	21.104033126652975	25.0297558306076	23.530375028822668
21	27.256762264609858	24.063601921471097	26.200543160553853	22.479092653365193
22	28.67649316473803	22.00745446018378	23.9729948904859	25.34305748459229
23	28.943076509058596	24.621753273141366	22.363139735307062	24.072030482492977
24	27.804618731031976	20.171232237560197	26.783859787275162	25.24028924413267
25	29.33145256014531	21.68698852933049	24.18093953169711	24.800619378827093
26	24.580272712112265	26.239314541254494	23.89840212544227	25.28201062119097
27	24.75191433692209	24.161252821310292	25.883388450102018	25.2034443916656
28	24.919582497250182	23.68949422011428	27.24050718263909	24.15041609999645
29	26.19301765964146	23.57823721462548	24.94679470854939	25.28195041718367
30	25.936428180532552	23.93482554985825	26.275015517582883	23.853730752026316
31	27.3579049968724	24.442345331389948	22.144117556752814	26.05563211498484
32	30.433306301734053	24.702667458951403	23.79160021649361	21.07242602282093
33	30.161605616793064	24.19255890510584	22.94573391394078	22.700101564160313
34	26.450510198859856	24.733552114695858	25.657503014715665	23.15843467172862
35	24.95594571765886	22.559103779065744	27.89733290227264	24.58761760100276
36	28.390584334074443	25.56785924784726	22.739776004970444	23.301780413107856
37	28.443925084541476	27.441648771025495	23.340009957742808	20.774416186690218
38	26.185913586780163	25.152812821527025	25.74907330981765	22.912200281875162
39	26.004940340838967	25.32884933886969	24.10995900709143	24.55625131319991
40	27.048516603362156	22.07668906857778	28.280290592702436	22.594503735357634
41	23.54139236215841	24.17269158269713	27.41828961619343	24.867626438951028
42	24.444211655616222	25.641368340759495	24.169259954281078	25.745160049343202
43	24.043553987040486	23.23880702147296	25.964603655948547	26.753035335538005
44	28.29696710272429	22.730564791853674	24.680151160221527	24.292316945200508
45	23.757946176413395	23.033571560590214	25.954128158678497	27.25435410431789
46	22.98396345857573	25.206454592030553	24.563656406097703	27.24592554329601
47	25.37165438805938	22.90678192121824	28.138991787571367	23.582571903151017
48	24.748302096484146	20.575261330544684	30.285505463814683	24.39093110915649
49	23.52561891224604	22.525750759022024	26.266285936524508	27.682344392207437
50	22.560127247189826	23.645244274749416	28.985038702146092	24.809589775914663
51	24.368475014433912	24.19141502896716	26.012345433736762	25.427764522862173
52	23.586184143588966	23.085888842933162	27.660129113514053	25.66779789996382
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	199.0
1	160.0
2	121.0
3	110.5
4	100.0
5	141.0
6	182.0
7	272.5
8	363.0
9	653.5
10	944.0
11	829.5
12	715.0
13	945.0
14	1186.0
15	1197.0
16	1253.5
17	1310.0
18	1425.5
19	1541.0
20	1765.5
21	1990.0
22	3218.5
23	4447.0
24	5786.5
25	7126.0
26	7681.0
27	8236.0
28	10978.0
29	13720.0
30	15370.5
31	17021.0
32	19844.5
33	22668.0
34	26593.5
35	30519.0
36	30826.0
37	31133.0
38	37110.5
39	50580.0
40	58072.0
41	68532.5
42	78993.0
43	82610.5
44	86228.0
45	91119.5
46	96011.0
47	108571.5
48	121132.0
49	137230.5
50	153329.0
51	158287.5
52	163246.0
53	145662.5
54	128079.0
55	117716.0
56	107353.0
57	109356.5
58	111360.0
59	105119.5
60	98879.0
61	89224.0
62	79569.0
63	72344.5
64	53456.5
65	41793.0
66	34763.0
67	27733.0
68	23703.0
69	19673.0
70	16965.0
71	14257.0
72	11662.5
73	9068.0
74	7398.0
75	5728.0
76	4591.0
77	3454.0
78	2675.5
79	1897.0
80	1573.0
81	1249.0
82	880.5
83	512.0
84	373.5
85	235.0
86	194.0
87	153.0
88	95.5
89	39.5
90	41.0
91	22.0
92	3.0
93	5.0
94	7.0
95	5.5
96	4.0
97	3.5
98	3.0
99	4.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1661019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.744512832303553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.49044721464558	11.635836851081459
2	12.109764493627226	4.055442139194302
3	4.942807007123487	2.4829468607513747
4	2.6798807455106735	1.7949319012898677
5	1.633676006861526	1.3677554430359624
6	0.9998018396991233	1.0044716840761603
7	0.702307484533082	0.8231857680890915
8	0.522535629543055	0.6999683643375599
9	0.4116106127000249	0.6202997267641106
>10	3.2528178261030605	12.477113185519645
>50	1.5429153733977727	18.937130447521938
>100	1.6999375624930848	40.9754041881497
>500	0.00610842074875927	0.6722970505249465
>1k	0.005030464146037045	2.017372481125958
>5k	3.593188675740747E-4	0.43584390853791943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCTT	7244	0.43611782887492556	Illumina Single End Adapter 1 (95% over 22bp)
GTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	4119	0.2479803060651323	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	3778	0.22745073957612766	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	3544	0.21336300186813034	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	3267	0.19668649184627027	No Hit
TTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	2762	0.16628346816020767	No Hit
ATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	2470	0.14870389802886058	No Hit
GGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	2404	0.14473043354711776	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	2173	0.13082330786101784	Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1983	0.11938454647418242	No Hit
TCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1959	0.1179396502990032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4573096394442207	0.0
2	0.0	0.0	0.0	0.9301519127716179	0.0
3	0.0	0.0	0.0	2.7245323503223022	0.0
4	0.0	0.0	0.0	3.103576780277649	0.0
5	0.0	0.0	0.0	3.759499439801712	0.0
6	0.0	0.0	0.0	5.760560234410323	0.0
7	0.0	0.0	0.0	6.0268425586943914	0.0
8	0.0	0.0	0.0	6.533158260080107	0.0
9	6.020400729913385E-5	0.0	0.0	6.858139491480832	0.0
10	6.020400729913385E-5	0.0	0.0	7.946567739441873	0.0
11	6.020400729913385E-5	0.0	0.0	8.881836992833916	0.0
12	6.020400729913385E-5	0.0	0.0	9.290260978351242	0.0
13	6.020400729913385E-5	0.0	0.0	9.501577043971201	0.0
14	6.020400729913385E-5	0.0	0.0	9.647872781708097	0.0
15	6.020400729913385E-5	0.0	0.0	9.839140912897443	0.0
16	6.020400729913385E-5	0.0	0.0	10.279653634305207	0.0
17	6.020400729913385E-5	0.0	0.0	10.834915193625118	0.0
18	6.020400729913385E-5	0.0	0.0	11.504744978835282	0.0
19	6.020400729913385E-5	0.0	0.0	11.934119958892703	0.0
20	6.020400729913385E-5	0.0	0.0	12.389202050066856	0.0
21	1.204080145982677E-4	0.0	0.0	13.05969407935731	0.0
22	1.204080145982677E-4	0.0	0.0	13.625732155983767	0.0
23	1.204080145982677E-4	0.0	0.0	14.23710385010647	0.0
24	1.204080145982677E-4	0.0	0.0	14.69314920539741	0.0
25	1.204080145982677E-4	0.0	0.0	15.106509919513263	0.0
26	1.204080145982677E-4	0.0	0.0	15.474296200103671	0.0
27	1.8061202189740153E-4	0.0	0.0	15.814027413292683	0.0
28	1.8061202189740153E-4	0.0	0.0	16.17139840062034	0.0
29	1.8061202189740153E-4	0.0	0.0	16.544603041867674	0.0
30	1.8061202189740153E-4	0.0	0.0	16.936591333392332	0.0
31	1.8061202189740153E-4	0.0	0.0	17.324967384479045	0.0
32	1.8061202189740153E-4	0.0	0.0	17.721470976551142	0.0
33	1.8061202189740153E-4	0.0	0.0	18.10183989466707	0.0
34	1.8061202189740153E-4	0.0	0.0	18.476730248118777	0.0
35	1.8061202189740153E-4	0.0	0.0	18.88100015713246	0.0
36	1.8061202189740153E-4	0.0	0.0	19.277503749204556	0.0
37	1.8061202189740153E-4	0.0	0.0	19.68159304619634	0.0
38	3.010200364956692E-4	0.0	0.0	20.067019100925396	0.0
39	4.214280510939369E-4	0.0	0.0	20.466954321413542	0.0
40	4.214280510939369E-4	0.0	0.0	20.897774197646143	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGATA	35	1.01994374E-7	46.000004	12
TCTATCG	35	1.01994374E-7	46.000004	1
CGAGTTG	60	0.0	46.0	1
GTCGACG	20	6.312271E-4	46.0	12
CCGATAA	20	6.312271E-4	46.0	46
AACCCGT	20	6.312271E-4	46.0	43
TAGCGAT	30	1.8619066E-6	46.0	14
ATTAACG	25	3.417817E-5	46.0	1
CTAAGCG	20	6.312271E-4	46.0	15
TCGATTG	30	1.8619066E-6	46.0	1
TTATACG	25	3.417817E-5	46.0	39
TACGCTA	30	1.8619066E-6	46.0	21
TCAATCG	30	1.8619066E-6	46.0	41
TACGCGA	20	6.312271E-4	46.0	39
CTACGTA	20	6.312271E-4	46.0	23
TTGACGA	20	6.312271E-4	46.0	28
TCTAACG	25	3.417817E-5	46.0	1
TCATGCG	45	3.110472E-10	46.0	1
TGTTACG	45	3.110472E-10	46.0	1
CATACCG	30	1.8619066E-6	46.0	28
>>END_MODULE