FastQCFastQC Report
Thu 26 May 2016
SRR1545646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545646_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1571827
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC71930.45762033608024294No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49420.31441119156243025No Hit
ATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG39290.24996389551776374No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT36020.22916007932170654No Hit
ATGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT27890.17743682988013312No Hit
ATGCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC27360.17406495753031345No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC25490.1621679739564214No Hit
ATTGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC23770.15122529387776135No Hit
ATGTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC20620.13118492047788977No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT20530.13061233838075054No Hit
ATATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC19760.1257135804385597No Hit
ATAGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC17150.10910869962152323No Hit
ATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC16700.1062457891358273No Hit
ATGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC16400.10433718214536332No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA16110.10249219538791482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAA551.8189894E-1245.00000433
ACGATTT551.8189894E-1245.00000417
CGTCGAT1250.045.00000445
GCTACGA2500.045.00000410
ATCGGAT750.045.0000041
TGATCGA351.2123019E-745.035
CGGACAA351.2123019E-745.026
GCCCCTA253.8914626E-545.011
CTTCGTC253.8914626E-545.042
CTAGATT207.034079E-445.023
CGGAATA253.8914626E-545.032
AGACTCG351.2123019E-745.040
GATACCG253.8914626E-545.044
CAAACGA502.1827873E-1145.032
ACACGCG253.8914626E-545.038
ACACGAG1550.045.028
CGTATAT253.8914626E-545.038
CGAGTTA253.8914626E-545.08
GTCGAAC351.2123019E-745.029
TACCAGA351.2123019E-745.017