##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545646_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1571827
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.937079589547704	31.0	31.0	34.0	31.0	34.0
2	32.59243860806565	34.0	31.0	34.0	31.0	34.0
3	32.54757425594547	34.0	31.0	34.0	31.0	34.0
4	36.00434271710564	37.0	35.0	37.0	35.0	37.0
5	35.77440392613182	37.0	35.0	37.0	35.0	37.0
6	35.78270636654034	37.0	35.0	37.0	35.0	37.0
7	35.76077201880359	37.0	35.0	37.0	33.0	37.0
8	35.7346864508626	37.0	35.0	37.0	33.0	37.0
9	37.91405161000543	39.0	38.0	39.0	35.0	39.0
10	37.84781658541303	39.0	38.0	39.0	35.0	39.0
11	37.70049057561678	39.0	38.0	39.0	35.0	39.0
12	37.25014330457487	39.0	37.0	39.0	34.0	39.0
13	36.97994308533954	39.0	37.0	39.0	33.0	39.0
14	37.56633904367338	40.0	35.0	41.0	33.0	41.0
15	37.384718547270154	40.0	35.0	41.0	32.0	41.0
16	37.45523839455615	40.0	35.0	41.0	32.0	41.0
17	37.5686115583967	40.0	35.0	41.0	33.0	41.0
18	37.63486757766599	39.0	35.0	41.0	33.0	41.0
19	37.59793539619818	39.0	35.0	41.0	33.0	41.0
20	37.57300580789107	39.0	35.0	41.0	33.0	41.0
21	37.55827645154333	39.0	35.0	41.0	33.0	41.0
22	37.48380069816844	39.0	35.0	41.0	33.0	41.0
23	37.3812995959479	39.0	35.0	41.0	32.0	41.0
24	37.34144088376138	39.0	35.0	41.0	32.0	41.0
25	37.28985378161846	39.0	35.0	41.0	32.0	41.0
26	37.18387265265198	39.0	35.0	41.0	32.0	41.0
27	37.08898371131174	39.0	35.0	41.0	32.0	41.0
28	36.981913403956035	39.0	35.0	41.0	32.0	41.0
29	36.93688936505099	39.0	35.0	41.0	31.0	41.0
30	36.82369497406521	39.0	35.0	41.0	31.0	41.0
31	36.76615047330272	39.0	35.0	41.0	31.0	41.0
32	36.668215395205706	38.0	35.0	41.0	31.0	41.0
33	36.54048759818988	38.0	35.0	41.0	31.0	41.0
34	36.454200748555664	38.0	35.0	41.0	30.0	41.0
35	36.35299749908864	38.0	35.0	41.0	30.0	41.0
36	36.33342346199677	38.0	35.0	41.0	30.0	41.0
37	36.1153046741149	38.0	35.0	41.0	30.0	41.0
38	36.0284000720181	38.0	35.0	40.0	30.0	41.0
39	36.01201595340963	38.0	35.0	40.0	30.0	41.0
40	35.971110052187676	38.0	35.0	40.0	30.0	41.0
41	35.9206948347369	38.0	35.0	40.0	30.0	41.0
42	35.7597318279938	38.0	35.0	40.0	29.0	41.0
43	35.68020653672446	38.0	35.0	40.0	29.0	41.0
44	35.57147510508472	37.0	35.0	40.0	29.0	41.0
45	35.54616443158185	37.0	34.0	40.0	29.0	41.0
46	35.48156126596629	37.0	34.0	40.0	29.0	41.0
47	35.39126252443812	37.0	34.0	40.0	28.0	41.0
48	35.3290018558022	37.0	34.0	40.0	28.0	41.0
49	35.285711468246824	37.0	34.0	40.0	28.0	41.0
50	35.19522250222194	36.0	34.0	40.0	28.0	41.0
51	35.05988954255144	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	6.0
11	17.0
12	24.0
13	21.0
14	48.0
15	51.0
16	112.0
17	208.0
18	402.0
19	764.0
20	1397.0
21	2385.0
22	3732.0
23	5478.0
24	7874.0
25	11242.0
26	15219.0
27	18626.0
28	21659.0
29	26154.0
30	31844.0
31	39101.0
32	49212.0
33	62931.0
34	102931.0
35	213113.0
36	112134.0
37	153047.0
38	229877.0
39	461953.0
40	258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.222827321327347	90.93443489646124	3.222364802233325	3.6203729799780766
2	3.8376996959589063	2.585208168583438	90.4911927330425	3.085899402415151
3	27.797015829350176	16.652341510866016	42.44582896209316	13.104813697690648
4	26.260014619929546	15.822479191412286	44.074061585657965	13.843444603000203
5	23.924579486164827	14.47608420010599	46.32297320252165	15.27636311120753
6	22.802573056704077	14.709188733874656	47.19259816761005	15.295640041811218
7	23.11494840081001	17.53647188908194	43.59786414153721	15.750715568570842
8	24.48125652505015	19.43012812478727	43.365523050564725	12.723092299597857
9	80.32798774928793	4.408564046806678	10.237258934984576	5.026189268920817
10	81.1353921264872	4.416007614069487	9.867689001397736	4.580911258045574
11	74.43325505923998	7.320462111924531	11.289410348594343	6.956872480241145
12	43.331931567532564	23.41580848274015	16.593301934627664	16.658958015099625
13	35.417256479243584	22.537340305262603	24.937413595771034	17.10798961972278
14	30.664888693221325	24.545767441327833	26.456092178083214	18.33325168736763
15	20.659398267112092	30.663743529027048	30.512200133984212	18.164658069876648
16	17.186815088428943	34.74790800768787	29.75766417042079	18.3076127334624
17	15.58574830436174	24.976985380706658	40.139531895049515	19.297734419882087
18	19.772786699808567	20.833463224642408	39.32875564550043	20.0649944300486
19	18.666685328601684	21.542001759735648	29.706831604241437	30.084481307421235
20	20.767361802539337	22.72648325801758	34.05578349271262	22.45037144673046
21	26.149506275181682	24.725303738897473	27.58751440203025	21.537675583890596
22	27.383420694516637	21.94910763080161	27.24485582700895	23.422615847672805
23	23.71138808533	26.32497087783834	28.26863261669382	21.69500842013784
24	27.769341027988446	21.939500975616273	24.73249282522822	25.558665171167057
25	25.074387957453332	26.972052267838638	23.614685331146493	24.338874443561537
26	24.101761835112896	20.38233215232974	33.72018676355604	21.795719249001323
27	23.945065201195806	24.052010812894803	29.653326988275424	22.349596997633963
28	21.69418135710864	28.798907258877726	26.06323723921271	23.443674144800923
29	21.833000705548383	27.905551946874564	27.401806941858105	22.85964040571895
30	21.04468239825375	24.810872952303274	32.830394184601744	21.314050464841234
31	20.443343955791573	23.421597923944557	31.697126973897255	24.43793114636662
32	22.201679955873008	27.405878636771096	29.628069755768287	20.76437165158761
33	25.847819130222344	25.781781328352295	27.40721466166442	20.96318487976094
34	26.10993449024606	24.272518540526406	27.613598697566587	22.00394827166094
35	25.28039027195741	26.151033160774055	26.536699013313807	22.03187755395473
36	23.464541581229994	23.995770526909133	27.62256915042177	24.917118741439104
37	21.997013666262255	23.719022513291858	31.61047621653019	22.6734876039157
38	24.933914482955185	27.037135766213456	25.447647864555066	22.58130188627629
39	22.935157622308306	27.539353885637542	28.73477806399814	20.790710428056013
40	22.532950509184534	27.31222965377233	28.51560636125986	21.639213475783276
41	22.080737892910605	27.62816773092713	25.852463407232474	24.438630968929786
42	24.687322459787243	23.309499073371306	28.153479994935832	23.849698471905622
43	20.956377514828286	24.454599647416668	28.634194475600687	25.95482836215436
44	21.463430771961544	24.34460026453293	26.496554646281044	27.695414317224476
45	20.739623380944593	23.69331993915361	27.94130651782925	27.625750162072542
46	21.976527951231276	25.402413878881074	28.910688008285902	23.710370161601755
47	19.245438588343376	28.538636885611457	27.77563943105698	24.44028509498819
48	21.229371934697646	26.328469990654185	27.506462225168548	24.935695849479618
49	22.602551044103457	24.431251021899993	29.101930428730387	23.864267505266167
50	20.939390912613156	21.98976095969849	33.325614078394125	23.74523404929423
51	20.370498788988865	22.746968973048563	30.278713878817452	26.60381835914512
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1010.0
1	1267.0
2	1524.0
3	4649.0
4	7774.0
5	5283.5
6	2793.0
7	2961.0
8	3129.0
9	3353.5
10	3578.0
11	3738.5
12	3899.0
13	3700.5
14	3502.0
15	4177.0
16	4852.0
17	5041.5
18	5231.0
19	5024.0
20	4817.0
21	4884.0
22	4951.0
23	5552.0
24	6153.0
25	8883.5
26	13536.5
27	15459.0
28	18658.5
29	21858.0
30	24998.5
31	28139.0
32	32056.0
33	35973.0
34	43715.5
35	51458.0
36	54183.0
37	56908.0
38	70266.5
39	83625.0
40	98262.0
41	112899.0
42	129948.0
43	146997.0
44	159378.5
45	171760.0
46	164938.0
47	158116.0
48	136045.0
49	113974.0
50	101759.5
51	89545.0
52	85345.0
53	81145.0
54	75669.0
55	70193.0
56	70027.0
57	69861.0
58	65020.0
59	60179.0
60	54183.5
61	48188.0
62	40128.0
63	32068.0
64	27250.5
65	22433.0
66	19116.5
67	15800.0
68	11945.0
69	8090.0
70	6486.0
71	4882.0
72	4676.5
73	4471.0
74	2863.0
75	905.0
76	555.0
77	750.5
78	946.0
79	503.0
80	60.0
81	64.0
82	68.0
83	42.0
84	16.0
85	16.0
86	16.0
87	17.0
88	18.0
89	29.0
90	40.0
91	20.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1571827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.014111286680015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.7261707853005	10.618905990512387
2	11.61726872538237	3.488459309803164
3	5.247227942644329	2.3634739283231694
4	2.8280875715949527	1.6984488611361304
5	1.7785002025774748	1.3351299982440579
6	1.171605450425423	1.0554368770060902
7	0.8030730627542809	0.8440199834869417
8	0.5577491762540463	0.6699286561865887
9	0.41677897102012396	0.5631809267559714
>10	2.5429449341289616	7.55805277150954
>50	0.5619097182457199	6.168854325442249
>100	1.7163555727987228	58.09415352340955
>500	0.02169371355953776	2.029504023757676
>1k	0.010208806380958946	3.053069414555218
>5k	4.2536693253995606E-4	0.459381409871262
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	7193	0.45762033608024294	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4942	0.31441119156243025	No Hit
ATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG	3929	0.24996389551776374	No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	3602	0.22916007932170654	No Hit
ATGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT	2789	0.17743682988013312	No Hit
ATGCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	2736	0.17406495753031345	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2549	0.1621679739564214	No Hit
ATTGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	2377	0.15122529387776135	No Hit
ATGTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	2062	0.13118492047788977	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2053	0.13061233838075054	No Hit
ATATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	1976	0.1257135804385597	No Hit
ATAGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	1715	0.10910869962152323	No Hit
ATACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	1670	0.1062457891358273	No Hit
ATGGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC	1640	0.10433718214536332	No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA	1611	0.10249219538791482	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25359024879964526	0.0
2	0.0	0.0	0.0	0.9604746578344818	0.0
3	0.0	0.0	0.0	1.3594371390744655	0.0
4	0.0	0.0	0.0	1.7674973136356609	0.0
5	0.0	0.0	0.0	3.254365779440104	0.0
6	6.362023301546545E-5	0.0	0.0	3.6038317193940554	0.0
7	6.362023301546545E-5	0.0	0.0	4.1433949155982175	0.0
8	6.362023301546545E-5	0.0	0.0	5.819151853225578	0.0
9	6.362023301546545E-5	0.0	0.0	6.047230388586021	0.0
10	6.362023301546545E-5	0.0	0.0	6.446829072156159	0.0
11	1.9086069904639632E-4	0.0	0.0	6.784207167837173	0.0
12	1.9086069904639632E-4	0.0	0.0	7.5777423342390735	0.0
13	1.9086069904639632E-4	0.0	0.0	8.306830204596308	0.0
14	1.9086069904639632E-4	0.0	0.0	8.63803713767482	0.0
15	3.1810116507732724E-4	0.0	0.0	8.822472193186655	0.0
16	3.8172139809279263E-4	0.0	0.0	8.96396359141305	0.0
17	3.8172139809279263E-4	0.0	0.0	9.148398646924884	0.0
18	4.453416311082581E-4	0.0	0.0	9.52293095868693	0.0
19	4.453416311082581E-4	0.0	0.0	10.022095306926271	0.0
20	4.453416311082581E-4	0.0	0.0	10.573110145073217	0.0
21	4.453416311082581E-4	0.0	0.0	10.929001728561731	0.0
22	4.453416311082581E-4	0.0	0.0	11.2855295143804	0.0
23	5.089618641237236E-4	0.0	0.0	11.823883926157269	0.0
24	5.089618641237236E-4	0.0	0.0	12.255292726235139	0.0
25	5.72582097139189E-4	0.0	0.0	12.743005432531698	0.0
26	8.270630292010507E-4	0.0	0.0	13.104559216758588	0.0
27	0.0010815439612629125	0.0	0.0	13.437420275895503	0.0
28	0.001272404660309309	0.0	0.0	13.725874412387622	0.0
29	0.0013360248933247743	0.0	0.0	13.99123440429513	0.0
30	0.0013996451263402396	0.0	0.0	14.294448434846837	0.0
31	0.0013996451263402396	0.0	0.0	14.611022714331794	0.0
32	0.0013996451263402396	0.0	0.0	14.982055913277987	0.0
33	0.0013996451263402396	0.0	0.0	15.314344390317764	0.0
34	0.0015268855923711705	0.0	0.0	15.634672263550632	0.0
35	0.001590505825386636	0.0	0.0	15.948383632549893	0.0
36	0.001590505825386636	0.0	0.0	16.271447175802425	0.0
37	0.0016541260584021014	0.0	0.0	16.65749474974027	0.0
38	0.0016541260584021014	0.0	0.0	17.011859447636414	0.0
39	0.0016541260584021014	0.0	0.0	17.352609415667246	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAA	55	1.8189894E-12	45.000004	33
ACGATTT	55	1.8189894E-12	45.000004	17
CGTCGAT	125	0.0	45.000004	45
GCTACGA	250	0.0	45.000004	10
ATCGGAT	75	0.0	45.000004	1
TGATCGA	35	1.2123019E-7	45.0	35
CGGACAA	35	1.2123019E-7	45.0	26
GCCCCTA	25	3.8914626E-5	45.0	11
CTTCGTC	25	3.8914626E-5	45.0	42
CTAGATT	20	7.034079E-4	45.0	23
CGGAATA	25	3.8914626E-5	45.0	32
AGACTCG	35	1.2123019E-7	45.0	40
GATACCG	25	3.8914626E-5	45.0	44
CAAACGA	50	2.1827873E-11	45.0	32
ACACGCG	25	3.8914626E-5	45.0	38
ACACGAG	155	0.0	45.0	28
CGTATAT	25	3.8914626E-5	45.0	38
CGAGTTA	25	3.8914626E-5	45.0	8
GTCGAAC	35	1.2123019E-7	45.0	29
TACCAGA	35	1.2123019E-7	45.0	17
>>END_MODULE