FastQCFastQC Report
Thu 26 May 2016
SRR1545645_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545645_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1261332
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT67920.5384783704845354Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT44400.3520088287619754No Hit
TGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC41960.33266419943361464No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC38490.3051535995281179No Hit
GTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC38240.3031715678346383No Hit
TTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC32570.2582190890265212No Hit
ATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC27920.22135329952780078No Hit
GGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC27630.21905414276336443No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC21690.17196106972628936No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC19980.15840397294288894No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT17400.13794940586617957No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC17050.13517456149530815No Hit
CTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC14120.11194515004772732No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGTC551.8189894E-1246.00000428
TTTAGCG1550.046.0000041
CGAAACG453.110472E-1046.00000433
GACCGTA351.0196709E-746.00000417
TAGCGCA351.0196709E-746.00000416
ATTACCG351.0196709E-746.00000416
GCAGTAA351.0196709E-746.00000410
CGACCGG453.110472E-1046.00000426
CACGACG351.0196709E-746.00000427
ACGGGTA551.8189894E-1246.0000045
CCGTAAG351.0196709E-746.00000435
CGGATGT351.0196709E-746.00000418
CGTAGAC351.0196709E-746.00000418
TATTACG351.0196709E-746.0000041
GTATCTC351.0196709E-746.00000420
CGTACAC453.110472E-1046.00000441
TCCCGAA453.110472E-1046.00000432
CGTAAGT351.0196709E-746.00000432
TAAGGTT453.110472E-1046.00000445
CGATGCG351.0196709E-746.0000041
Produced by FastQC (version 0.11.5)