##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545645_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1261332
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58773502931821	33.0	31.0	34.0	31.0	34.0
2	32.97846641486936	34.0	31.0	34.0	31.0	34.0
3	33.042403586050305	34.0	33.0	34.0	31.0	34.0
4	36.42089870073858	37.0	37.0	37.0	35.0	37.0
5	36.39528371594473	37.0	37.0	37.0	35.0	37.0
6	36.40804007192396	37.0	37.0	37.0	35.0	37.0
7	36.48512287010874	37.0	37.0	37.0	35.0	37.0
8	36.52978835072764	37.0	37.0	37.0	35.0	37.0
9	38.35306326962291	39.0	39.0	39.0	37.0	39.0
10	38.12438517376868	39.0	39.0	39.0	35.0	39.0
11	37.933581325138825	39.0	38.0	39.0	35.0	39.0
12	37.47748332714939	39.0	37.0	39.0	35.0	39.0
13	37.370801660466874	39.0	37.0	39.0	35.0	39.0
14	38.69788921552771	41.0	38.0	41.0	35.0	41.0
15	38.750822939559136	41.0	38.0	41.0	35.0	41.0
16	38.81204314169465	41.0	38.0	41.0	35.0	41.0
17	38.759554978387925	40.0	37.0	41.0	35.0	41.0
18	38.778459596680335	40.0	37.0	41.0	35.0	41.0
19	38.75666755461687	40.0	37.0	41.0	35.0	41.0
20	38.67766932100351	40.0	37.0	41.0	35.0	41.0
21	38.607991393225575	40.0	37.0	41.0	35.0	41.0
22	38.68819153085786	40.0	37.0	41.0	35.0	41.0
23	38.687304373471854	40.0	37.0	41.0	35.0	41.0
24	38.652814643567275	40.0	37.0	41.0	35.0	41.0
25	38.59618086277047	40.0	36.0	41.0	35.0	41.0
26	38.44830782062137	40.0	36.0	41.0	35.0	41.0
27	38.38401467654828	40.0	36.0	41.0	35.0	41.0
28	38.27400795349678	40.0	36.0	41.0	35.0	41.0
29	38.28968978825559	40.0	36.0	41.0	35.0	41.0
30	38.23363079664989	40.0	36.0	41.0	35.0	41.0
31	38.12644410829187	40.0	35.0	41.0	34.0	41.0
32	38.06125032901726	40.0	35.0	41.0	34.0	41.0
33	37.98902667973222	40.0	35.0	41.0	34.0	41.0
34	37.97813264073218	40.0	35.0	41.0	34.0	41.0
35	37.80596861096048	40.0	35.0	41.0	34.0	41.0
36	37.73122064611062	40.0	35.0	41.0	34.0	41.0
37	37.69287546815588	40.0	35.0	41.0	34.0	41.0
38	37.6555490544916	40.0	35.0	41.0	33.0	41.0
39	37.57838697503909	40.0	35.0	41.0	33.0	41.0
40	37.4297631392845	39.0	35.0	41.0	33.0	41.0
41	37.32821969156416	39.0	35.0	41.0	33.0	41.0
42	37.240582178205266	39.0	35.0	41.0	33.0	41.0
43	37.170614873800076	39.0	35.0	41.0	33.0	41.0
44	37.04991548616859	39.0	35.0	41.0	33.0	41.0
45	36.967944205015016	39.0	35.0	41.0	33.0	41.0
46	36.939586881170065	39.0	35.0	41.0	33.0	41.0
47	36.920193890268386	39.0	35.0	41.0	33.0	41.0
48	36.83691288257176	38.0	35.0	41.0	33.0	41.0
49	36.78114802446937	38.0	35.0	41.0	33.0	41.0
50	36.68131388088148	38.0	35.0	41.0	33.0	41.0
51	36.56807248210622	38.0	35.0	40.0	32.0	41.0
52	36.21077321434801	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	5.0
13	9.0
14	13.0
15	30.0
16	40.0
17	64.0
18	125.0
19	310.0
20	504.0
21	886.0
22	1281.0
23	1892.0
24	2686.0
25	3635.0
26	4654.0
27	5834.0
28	7530.0
29	9369.0
30	12165.0
31	15681.0
32	20618.0
33	28458.0
34	67921.0
35	164030.0
36	71566.0
37	100711.0
38	182370.0
39	558014.0
40	930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.68113391240371	14.482467740452156	45.48897514690819	12.34742320023594
2	25.111786587512245	13.106620620106362	49.214084792901474	12.567507999479915
3	25.320058477863082	13.896341328056373	45.339688519755306	15.443911674325236
4	23.281102834146758	14.298852324368209	47.22380784757701	15.196236993908027
5	23.26667364341823	17.405726644531335	43.230093266483365	16.097506445567067
6	23.399073360542662	19.236727522967783	44.569550284936874	12.794648831552676
7	84.10259947420663	2.982006323473915	7.560182410340814	5.355211791978638
8	85.58206721148753	2.8980474609381193	7.557249003434465	3.9626363241398774
9	78.09482356746678	5.024767468041721	9.624666622269157	7.255742342222349
10	44.25504149581553	22.816673167730624	14.337065895418494	18.59121944103535
11	35.25645904488271	21.19188286668379	25.748177323654676	17.80348076477882
12	30.877516783844385	24.08969248381869	25.691649779756638	19.34114095258029
13	21.046877427988825	29.081320381945435	31.778151985361507	18.093650204704232
14	17.041667063073003	35.155692553586206	29.425638927736706	18.377001455604073
15	15.536274351241385	24.83905902648946	38.809766183685184	20.814900438583972
16	19.811833839147823	21.752242867064343	37.33275616570419	21.103167128083644
17	18.87266794150945	22.487655906613007	27.8670484852521	30.77262766662544
18	20.57301329071172	23.317492936039045	31.808992398512046	24.30050137473718
19	25.49083032857329	25.278118687229057	27.201323680046173	22.029727304151482
20	27.336260397738265	21.296534140099514	25.9887959712431	25.378409490919125
21	23.35499297567968	27.200213742297823	27.823760913066504	21.62103236895599
22	28.046224150342653	21.872353987689205	23.72063818249279	26.360783679475347
23	25.483060764334848	27.037766424700237	21.965985164889183	25.513187646075735
24	24.205839541056598	19.792330647283983	31.74865935376253	24.25317045789689
25	26.504996305492924	23.652218448433878	26.704626537660186	23.138158708413012
26	22.07277703253386	28.38546869499862	25.054466230936818	24.4872880415307
27	23.45583874824392	26.20515455090333	25.13501599895983	25.20399070189292
28	21.47277639828372	23.747197407185418	32.42310509841976	22.356921096111098
29	21.28622757529342	21.980017949279016	29.697811519885324	27.035942955542236
30	23.472804939540104	25.468076604732143	29.469481468796477	21.589636986931275
31	26.4395892596081	25.003329813245045	26.282929474555473	22.274151452591386
32	26.357533147498042	23.476451877856107	26.786365524699285	23.379649449946566
33	25.066596264900916	25.887950198678855	27.20552558723635	21.83992794918388
34	23.73348174786654	22.916012596207818	28.257667291403056	25.092838364522585
35	21.63276599658139	22.4184433598767	32.66142458924375	23.287366054298158
36	24.94149042440848	25.75364773112868	25.3631874875132	23.941674356949637
37	23.659750168869103	25.721300973891093	29.384729793583293	21.234219063656514
38	22.04740702685732	26.003621568310326	29.924080258012957	22.024891146819396
39	22.818972324495057	26.722544104169245	26.302274103883832	24.15620946745187
40	25.62917614077816	22.802481820805305	28.377144161886008	23.191197876530527
41	21.27948866753559	23.95689636035556	28.650268129247493	26.113346842861358
42	22.034404898948097	23.952456609362166	25.940672241725416	28.07246624996432
43	21.14169782420489	22.464030088826732	28.396964478820802	27.997307608147576
44	25.01379494058662	23.522038606806138	26.74648704702648	24.717679405580768
45	20.13347794236569	25.8469617832577	27.86023029622653	26.15932997815008
46	21.55618029194534	24.656949954492553	27.206873368787914	26.57999638477419
47	23.38202788797874	23.018285431591366	29.63953978809703	23.96014689233287
48	20.50530708806246	20.312732888723982	35.164334211769784	24.017625811443775
49	20.847167914553822	20.80927146857449	29.532747920452344	28.81081269641934
50	19.90982548607345	23.641356914753608	31.712348533137984	24.736469066034953
51	22.70678933064411	24.36590842062201	28.760944779011393	24.16635746972248
52	21.011993670183585	20.917569680306215	31.02228437873613	27.04815227077407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	515.0
1	376.5
2	238.0
3	197.0
4	156.0
5	205.5
6	255.0
7	364.0
8	473.0
9	546.5
10	620.0
11	685.0
12	750.0
13	754.0
14	1172.5
15	1587.0
16	1414.0
17	1241.0
18	1482.5
19	1724.0
20	1891.5
21	2059.0
22	2248.0
23	2437.0
24	2848.5
25	3260.0
26	5456.0
27	7652.0
28	8338.5
29	9025.0
30	11563.0
31	14101.0
32	17308.0
33	20515.0
34	22266.5
35	24018.0
36	29271.0
37	34524.0
38	36677.5
39	48115.0
40	57399.0
41	73974.5
42	90550.0
43	96054.0
44	101558.0
45	121526.0
46	141494.0
47	142547.5
48	143601.0
49	125946.5
50	108292.0
51	93151.5
52	78011.0
53	72228.5
54	66446.0
55	62872.0
56	59298.0
57	56211.0
58	53124.0
59	52231.5
60	51339.0
61	48822.0
62	46305.0
63	38700.5
64	27234.0
65	23372.0
66	19825.0
67	16278.0
68	13454.0
69	10630.0
70	8483.0
71	6336.0
72	6028.5
73	5721.0
74	4263.0
75	2805.0
76	2111.0
77	1417.0
78	982.5
79	548.0
80	667.5
81	787.0
82	449.0
83	111.0
84	68.0
85	25.0
86	18.0
87	11.0
88	8.0
89	5.0
90	5.0
91	5.5
92	6.0
93	4.0
94	2.0
95	2.5
96	3.0
97	6.0
98	9.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1261332.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.31540719806368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.00694565900311	10.26238657866158
2	12.186205256600912	3.7327339140805416
3	5.69419335325952	2.616266696090317
4	3.279116923579703	2.0088404373874007
5	2.0977253458874334	1.6063758930982508
6	1.4376495409276748	1.3210912876449994
7	1.0207627835966768	1.0943378378388444
8	0.7222428854719392	0.8849155109525777
9	0.5992172287998484	0.8259530273248472
>10	3.3866300124426347	10.11240614461332
>50	0.7318674897581999	8.257396837973083
>100	1.8167107305941943	52.55536989593128
>500	0.012957993798817366	1.2983847594862201
>1k	0.007256476527337724	2.8843733330846053
>5k	5.183197519526946E-4	0.5391678458321354
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCTT	6792	0.5384783704845354	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	4440	0.3520088287619754	No Hit
TGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	4196	0.33266419943361464	No Hit
GCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3849	0.3051535995281179	No Hit
GTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3824	0.3031715678346383	No Hit
TTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	3257	0.2582190890265212	No Hit
ATCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2792	0.22135329952780078	No Hit
GGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2763	0.21905414276336443	No Hit
AGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	2169	0.17196106972628936	No Hit
ACCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1998	0.15840397294288894	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT	1740	0.13794940586617957	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1705	0.13517456149530815	No Hit
CTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC	1412	0.11194515004772732	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5649583139094227	0.0
2	0.0	0.0	0.0	1.2032518004775903	0.0
3	0.0	0.0	0.0	3.8193750733351726	0.0
4	0.0	0.0	0.0	4.415570206733833	0.0
5	0.0	0.0	0.0	5.3253227540409664	0.0
6	0.0	0.0	0.0	8.227175715830567	0.0
7	0.0	0.0	0.0	8.615495365217088	0.0
8	0.0	0.0	0.0	9.323556367395737	0.0
9	0.0	0.0	0.0	9.800512474114665	0.0
10	0.0	0.0	0.0	11.195466379985602	0.0
11	0.0	0.0	0.0	12.422106154446253	0.0
12	0.0	0.0	0.0	12.984765311591238	0.0
13	0.0	0.0	0.0	13.274141938839259	0.0
14	0.0	0.0	0.0	13.436509975169107	0.0
15	0.0	0.0	0.0	13.688307281508754	0.0
16	0.0	0.0	0.0	14.264285691633923	0.0
17	0.0	0.0	0.0	15.081279155686211	0.0
18	0.0	0.0	0.0	15.969229354365067	0.0
19	7.928126773918366E-5	0.0	0.0	16.504457192872298	0.0
20	7.928126773918366E-5	0.0	0.0	17.07028760072685	0.0
21	7.928126773918366E-5	0.0	0.0	17.895447035356273	0.0
22	7.928126773918366E-5	0.0	0.0	18.57092343649412	0.0
23	7.928126773918366E-5	0.0	0.0	19.317039447187575	0.0
24	3.1712507095673465E-4	0.0	0.0	19.863683788249247	0.0
25	6.342501419134693E-4	0.0	0.0	20.343652583142266	0.0
26	6.342501419134693E-4	0.0	0.0	20.77724183640786	0.0
27	6.342501419134693E-4	0.0	0.0	21.167622798755602	0.0
28	6.342501419134693E-4	0.0	0.0	21.586227892418492	0.0
29	6.342501419134693E-4	0.0	0.0	22.024732584283917	0.0
30	6.342501419134693E-4	0.0	0.0	22.528010071892254	0.0
31	6.342501419134693E-4	0.0	0.0	22.98371879885708	0.0
32	6.342501419134693E-4	0.0	0.0	23.405098736890842	0.0
33	6.342501419134693E-4	0.0	0.0	23.833217582682433	0.0
34	6.342501419134693E-4	0.0	0.0	24.23105098419766	0.0
35	6.342501419134693E-4	0.0	0.0	24.72322909432251	0.0
36	6.342501419134693E-4	0.0	0.0	25.171326819584376	0.0
37	6.342501419134693E-4	0.0	0.0	25.594054539169704	0.0
38	6.342501419134693E-4	0.0	0.0	26.0082198818392	0.0
39	7.135314096526529E-4	0.0	0.0	26.417231942105648	0.0
40	7.135314096526529E-4	0.0	0.0	26.85097975790672	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGTC	55	1.8189894E-12	46.000004	28
TTTAGCG	155	0.0	46.000004	1
CGAAACG	45	3.110472E-10	46.000004	33
GACCGTA	35	1.0196709E-7	46.000004	17
TAGCGCA	35	1.0196709E-7	46.000004	16
ATTACCG	35	1.0196709E-7	46.000004	16
GCAGTAA	35	1.0196709E-7	46.000004	10
CGACCGG	45	3.110472E-10	46.000004	26
CACGACG	35	1.0196709E-7	46.000004	27
ACGGGTA	55	1.8189894E-12	46.000004	5
CCGTAAG	35	1.0196709E-7	46.000004	35
CGGATGT	35	1.0196709E-7	46.000004	18
CGTAGAC	35	1.0196709E-7	46.000004	18
TATTACG	35	1.0196709E-7	46.000004	1
GTATCTC	35	1.0196709E-7	46.000004	20
CGTACAC	45	3.110472E-10	46.000004	41
TCCCGAA	45	3.110472E-10	46.000004	32
CGTAAGT	35	1.0196709E-7	46.000004	32
TAAGGTT	45	3.110472E-10	46.000004	45
CGATGCG	35	1.0196709E-7	46.000004	1
>>END_MODULE