SRR1545644_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1545644_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1690347
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	6143	0.36341650560506217	TruSeq Adapter, Index 27 (100% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3803	0.22498339098421805	No Hit
ATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2593	0.15340045564608923	TruSeq Adapter, Index 27 (100% over 23bp)
ATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2531	0.14973256970314378	TruSeq Adapter, Index 27 (100% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	2276	0.13464690977651333	TruSeq Adapter, Index 27 (100% over 21bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2080	0.12305165744075033	TruSeq Adapter, Index 27 (100% over 25bp)
ATTGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	2027	0.1199162065540389	TruSeq Adapter, Index 27 (95% over 22bp)
ATGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1902	0.11252127521745535	TruSeq Adapter, Index 27 (100% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1784	0.10554046003572047	TruSeq Adapter, Index 27 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	20	7.034229E-4	45.000004	38
GTCGAAC	30	2.1661108E-6	45.000004	42
AACCCGT	30	2.1661108E-6	45.000004	35
ATTAACG	35	1.2123746E-7	45.000004	1
CGTCTAC	30	2.1661108E-6	45.000004	28
CAATTCG	35	1.2123746E-7	45.000004	3
CCCACGT	20	7.034229E-4	45.000004	22
GTAATCG	30	2.1661108E-6	45.000004	43
CCGTAGA	20	7.034229E-4	45.000004	41
CTACGAC	20	7.034229E-4	45.000004	39
TACGACA	20	7.034229E-4	45.000004	20
GTCACGA	20	7.034229E-4	45.000004	36
GTCCGTT	35	1.2123746E-7	45.000004	13
GTCGTAC	20	7.034229E-4	45.000004	12
CGAATCG	20	7.034229E-4	45.000004	21
TTAACGA	30	2.1661108E-6	45.000004	2
ACTCGAT	20	7.034229E-4	45.000004	37
TCGTAGC	80	0.0	45.000004	2
TTACGAG	40	6.8175723E-9	45.000004	3
TACTAAT	20	7.034229E-4	45.000004	15