##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545644_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1690347
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85560242956032	31.0	31.0	34.0	31.0	34.0
2	32.50308131998933	33.0	31.0	34.0	31.0	34.0
3	32.48644272448202	34.0	31.0	34.0	31.0	34.0
4	35.988537856428295	37.0	35.0	37.0	35.0	37.0
5	35.76559664968199	37.0	35.0	37.0	35.0	37.0
6	35.75876669109952	37.0	35.0	37.0	35.0	37.0
7	35.748149936078214	37.0	35.0	37.0	33.0	37.0
8	35.71832529060601	37.0	35.0	37.0	33.0	37.0
9	37.8655506827888	39.0	38.0	39.0	35.0	39.0
10	37.796412807547796	39.0	38.0	39.0	35.0	39.0
11	37.62960741196926	39.0	37.0	39.0	35.0	39.0
12	37.163197260680796	39.0	37.0	39.0	33.0	39.0
13	36.92378310488911	39.0	37.0	39.0	33.0	39.0
14	37.669218805369546	40.0	36.0	41.0	33.0	41.0
15	37.567561571677295	40.0	36.0	41.0	32.0	41.0
16	37.598601352266726	40.0	36.0	41.0	33.0	41.0
17	37.67453901477034	40.0	36.0	41.0	33.0	41.0
18	37.740261023328344	40.0	36.0	41.0	33.0	41.0
19	37.716659952069016	40.0	35.0	41.0	33.0	41.0
20	37.64606557115196	40.0	36.0	41.0	33.0	41.0
21	37.62749541957953	40.0	36.0	41.0	33.0	41.0
22	37.54117763985738	40.0	35.0	41.0	32.0	41.0
23	37.465869433909134	39.0	35.0	41.0	32.0	41.0
24	37.40711167588667	39.0	35.0	41.0	32.0	41.0
25	37.33243765924985	39.0	35.0	41.0	32.0	41.0
26	37.24400315438191	39.0	35.0	41.0	32.0	41.0
27	37.1502839357836	39.0	35.0	41.0	32.0	41.0
28	37.07778639533776	39.0	35.0	41.0	31.0	41.0
29	37.01281038745299	39.0	35.0	41.0	31.0	41.0
30	36.9581411390679	39.0	35.0	41.0	31.0	41.0
31	36.883777117952704	39.0	35.0	41.0	31.0	41.0
32	36.73637188103981	39.0	35.0	41.0	31.0	41.0
33	36.68210846648647	39.0	35.0	41.0	31.0	41.0
34	36.60974640118272	39.0	35.0	41.0	31.0	41.0
35	36.508660647784154	39.0	35.0	41.0	30.0	41.0
36	36.42167495786368	39.0	35.0	41.0	30.0	41.0
37	36.27672542974904	38.0	35.0	41.0	30.0	41.0
38	36.18459050124028	38.0	35.0	41.0	30.0	41.0
39	36.121773813305786	38.0	35.0	41.0	30.0	41.0
40	36.0743823605449	38.0	35.0	41.0	30.0	41.0
41	35.99271096408015	38.0	35.0	40.0	30.0	41.0
42	35.84284351082943	38.0	35.0	40.0	29.0	41.0
43	35.723130812785776	38.0	35.0	40.0	29.0	41.0
44	35.63934032479722	38.0	35.0	40.0	29.0	41.0
45	35.599674504702286	38.0	34.0	40.0	29.0	41.0
46	35.53006394545025	37.0	34.0	40.0	29.0	41.0
47	35.44951302898162	37.0	34.0	40.0	28.0	41.0
48	35.375483850357355	37.0	34.0	40.0	28.0	41.0
49	35.360076363018955	37.0	34.0	40.0	28.0	41.0
50	35.27684493183944	37.0	34.0	40.0	28.0	41.0
51	35.0882948885643	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	13.0
10	19.0
11	21.0
12	30.0
13	23.0
14	33.0
15	56.0
16	120.0
17	228.0
18	506.0
19	918.0
20	1746.0
21	2827.0
22	4182.0
23	5869.0
24	8648.0
25	12338.0
26	16398.0
27	20389.0
28	23979.0
29	28198.0
30	33918.0
31	41586.0
32	51751.0
33	66559.0
34	106025.0
35	197335.0
36	126257.0
37	170701.0
38	257910.0
39	511516.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.4967062975826857	90.10948639539691	3.6090222895062376	3.7847850175141557
2	3.6216232525037757	3.1037414211401564	89.91189383008341	3.3627414962726587
3	28.00643891461339	15.68186887071116	42.78157088455802	13.530121330117426
4	27.984135801702255	15.621940347159487	42.50866833851275	13.885255512625513
5	24.536618812587	14.662255738023022	45.780245121267996	15.020880328121978
6	22.653632656490057	15.025790562529467	46.47708429097694	15.843492490003532
7	22.189290128003304	17.900762387841077	44.067342385912475	15.842605098243142
8	26.648197086160412	19.20156038967147	42.2523895981121	11.89785292605601
9	81.15102993645684	4.905501651436066	9.523133415801608	4.420334996305493
10	81.78036817292545	5.092208877822127	9.234908572026928	3.892514377225505
11	75.39363219504635	7.049321825636985	10.735488038846462	6.821557940470211
12	42.13637791530378	24.11954468520369	16.69148405623224	17.052593343260288
13	34.48173659017941	23.944787667857547	24.150425918465263	17.42304982349778
14	29.330368261664617	24.20473429420113	27.591021251849472	18.87387619228478
15	22.13137302577518	27.44667219215936	31.645750842874275	18.776203939191184
16	18.758101147279227	33.11142623378514	28.655358929261272	19.47511368967437
17	16.674682772235524	25.029417036856927	38.49103172307225	19.804868467835302
18	20.404804457309652	21.194997240211624	37.72184054516617	20.67835775731255
19	22.16988582817611	20.584412549612594	29.017237289148323	28.228464333062973
20	22.285838351533737	23.53055319410748	31.758567915345193	22.425040539013587
21	26.939734859173885	24.269158936005446	27.21405723203579	21.57704897278488
22	28.132271066236697	22.78739217450618	25.823928459659466	23.256408299597656
23	26.169952086760883	24.389666737066413	27.949468363596353	21.49091281257635
24	28.717121395784417	21.942417740262798	24.21556047367789	25.124900390274895
25	26.59885810428273	25.207901099596707	24.555904793512813	23.637336002607746
26	26.208819845865968	20.87813922230169	30.0970747426416	22.81596618919074
27	26.37736512088938	23.374135606476067	27.04781917558939	23.20068009704516
28	23.715603955874148	27.070240607401914	24.34263497376574	24.871520462958195
29	21.451275980612262	27.26392864896971	25.98590703565599	25.298888334762033
30	21.769376346986743	26.79787049641287	29.784002929575998	21.64875022702439
31	21.533507617075074	24.130370864680447	28.502905024826262	25.833216493418217
32	21.85515755049111	25.83794924947363	27.223226946893153	25.083666253142106
33	23.742935622094162	26.560581939684575	25.781097017357972	23.91538542086329
34	25.554989596810596	24.396174276642608	26.555080110770156	23.493756015776643
35	25.67324933874524	25.394253369278612	25.43850463839673	23.49399265357941
36	22.321866457005573	24.921746836596274	27.74282440232686	25.013562304071296
37	22.796976005518392	24.08387153643601	29.462707952864115	23.656444505181483
38	24.878087161985082	26.13546212700706	25.506774644496073	23.479676066511786
39	23.104309351866807	28.06003737694095	26.107420547378734	22.728232723813512
40	23.32148369535959	25.7615152391787	27.437916593456848	23.47908447200486
41	22.773962979198945	27.19051177066011	26.020160357607047	24.0153648925339
42	24.788519753636386	24.37653333901264	28.092220118117762	22.742726789233217
43	22.124155572790677	24.355590893467436	27.96124109428419	25.559012439457696
44	22.167460290697708	25.435487506411402	25.314328951392824	27.082723251498063
45	22.49780666336557	23.96188474910773	26.537746391717203	27.0025621958095
46	23.978331076400288	25.352368478188207	26.904416667110365	23.76488377830114
47	21.592548748866356	25.937159648285235	26.015960036607865	26.45433156624054
48	19.99193065092552	27.195717802321063	26.98114647465875	25.831205072094665
49	22.75651094124461	24.919084661315104	28.311524201835482	24.012880195604808
50	22.525374967388352	22.752606417498892	30.369563172532033	24.352455442580727
51	21.31083144466787	24.044234704471922	27.741996170017163	26.90293768084305
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	687.0
1	1482.0
2	2277.0
3	4766.0
4	7255.0
5	5224.0
6	3193.0
7	3299.0
8	3405.0
9	3498.5
10	3592.0
11	3875.5
12	4159.0
13	4503.5
14	4848.0
15	4577.0
16	4306.0
17	4033.5
18	3761.0
19	4191.0
20	4621.0
21	4414.0
22	4207.0
23	5683.0
24	7159.0
25	10523.5
26	15349.5
27	16811.0
28	19972.0
29	23133.0
30	25292.0
31	27451.0
32	33357.0
33	39263.0
34	43180.0
35	47097.0
36	51277.5
37	55458.0
38	67122.0
39	78786.0
40	90726.0
41	102666.0
42	110958.5
43	119251.0
44	130445.5
45	141640.0
46	152042.5
47	162445.0
48	150628.5
49	138812.0
50	126798.0
51	114784.0
52	109903.0
53	105022.0
54	100249.0
55	95476.0
56	95354.5
57	95233.0
58	86630.5
59	78028.0
60	71678.5
61	65329.0
62	55304.0
63	45279.0
64	34940.5
65	24602.0
66	21730.5
67	18859.0
68	14742.5
69	10626.0
70	9344.0
71	8062.0
72	6250.0
73	4438.0
74	3686.0
75	1970.5
76	1007.0
77	622.5
78	238.0
79	194.5
80	151.0
81	102.0
82	53.0
83	42.0
84	31.0
85	23.0
86	15.0
87	7.5
88	0.0
89	3.0
90	6.0
91	4.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1690347.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.983619315604189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.12625607543785	11.688222390851866
2	10.76555665752278	3.441451186688247
3	4.436079662760257	2.1271382574976125
4	2.419364326451447	1.5468079351901225
5	1.4996250492115955	1.198471795137117
6	0.9608160604286884	0.9214390885326422
7	0.6977038087653603	0.7806282452046837
8	0.4774828678603256	0.6105523511681907
9	0.36777633354674233	0.529055721782982
>10	2.4601876794734396	8.649631195143451
>50	0.9532731441026242	11.312770393091045
>100	1.8183973211247757	53.92735180545956
>500	0.012273902965252102	1.3546622397574368
>1k	0.004835173895402343	1.5466227598863158
>5k	3.719364534924879E-4	0.3651946346087653
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	6143	0.36341650560506217	TruSeq Adapter, Index 27 (100% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3803	0.22498339098421805	No Hit
ATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2593	0.15340045564608923	TruSeq Adapter, Index 27 (100% over 23bp)
ATGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT	2531	0.14973256970314378	TruSeq Adapter, Index 27 (100% over 22bp)
ATGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	2276	0.13464690977651333	TruSeq Adapter, Index 27 (100% over 21bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	2080	0.12305165744075033	TruSeq Adapter, Index 27 (100% over 25bp)
ATTGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	2027	0.1199162065540389	TruSeq Adapter, Index 27 (95% over 22bp)
ATGTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	1902	0.11252127521745535	TruSeq Adapter, Index 27 (100% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1784	0.10554046003572047	TruSeq Adapter, Index 27 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13932050638123414	0.0
2	0.0	0.0	0.0	0.6896808761751285	0.0
3	0.0	0.0	0.0	0.9268511140020362	0.0
4	0.0	0.0	0.0	1.2280910369291038	0.0
5	0.0	0.0	0.0	2.4082037593464536	0.0
6	0.0	0.0	0.0	2.688619555629702	0.0
7	0.0	0.0	0.0	3.1155141518279974	0.0
8	0.0	0.0	0.0	4.367860563541095	0.0
9	0.0	0.0	0.0	4.531673082509094	0.0
10	0.0	0.0	0.0	4.839124747758892	0.0
11	5.915945069266843E-5	0.0	0.0	5.0744610426143275	0.0
12	5.915945069266843E-5	0.0	0.0	5.727167262106538	0.0
13	5.915945069266843E-5	0.0	0.0	6.289714478743122	0.0
14	5.915945069266843E-5	0.0	0.0	6.529132775696351	0.0
15	5.915945069266843E-5	0.0	0.0	6.6686899198803555	0.0
16	5.915945069266843E-5	0.0	0.0	6.768136956494732	0.0
17	5.915945069266843E-5	0.0	0.0	6.902488069017782	5.915945069266843E-5
18	5.915945069266843E-5	0.0	0.0	7.190831231693847	5.915945069266843E-5
19	5.915945069266843E-5	0.0	0.0	7.5495741406941885	5.915945069266843E-5
20	1.1831890138533686E-4	0.0	0.0	7.968363892147589	5.915945069266843E-5
21	1.1831890138533686E-4	0.0	0.0	8.210444364381988	5.915945069266843E-5
22	1.1831890138533686E-4	0.0	0.0	8.489085377144455	5.915945069266843E-5
23	1.774783520780053E-4	0.0	0.0	8.897285586923868	5.915945069266843E-5
24	1.774783520780053E-4	0.0	0.0	9.255259423065205	5.915945069266843E-5
25	2.366378027706737E-4	0.0	0.0	9.665116097464011	5.915945069266843E-5
26	2.366378027706737E-4	0.0	0.0	9.950264649802673	5.915945069266843E-5
27	2.366378027706737E-4	0.0	0.0	10.221865687932715	5.915945069266843E-5
28	2.366378027706737E-4	0.0	0.0	10.47074949699677	5.915945069266843E-5
29	2.366378027706737E-4	0.0	0.0	10.720757335623988	5.915945069266843E-5
30	2.366378027706737E-4	0.0	0.0	10.957690935648124	5.915945069266843E-5
31	2.366378027706737E-4	0.0	0.0	11.203143496572006	5.915945069266843E-5
32	2.9579725346334215E-4	0.0	0.0	11.49633773420487	5.915945069266843E-5
33	2.9579725346334215E-4	0.0	0.0	11.77213909333409	5.915945069266843E-5
34	3.549567041560106E-4	0.0	0.0	12.055572021602664	5.915945069266843E-5
35	3.549567041560106E-4	0.0	0.0	12.331077583478422	5.915945069266843E-5
36	4.14116154848679E-4	0.0	0.0	12.5999572868766	5.915945069266843E-5
37	4.14116154848679E-4	0.0	0.0	12.902557877169599	5.915945069266843E-5
38	4.732756055413474E-4	0.0	0.0	13.190309445338738	5.915945069266843E-5
39	5.324350562340159E-4	0.0	0.0	13.489301309139485	5.915945069266843E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTACG	20	7.034229E-4	45.000004	38
GTCGAAC	30	2.1661108E-6	45.000004	42
AACCCGT	30	2.1661108E-6	45.000004	35
ATTAACG	35	1.2123746E-7	45.000004	1
CGTCTAC	30	2.1661108E-6	45.000004	28
CAATTCG	35	1.2123746E-7	45.000004	3
CCCACGT	20	7.034229E-4	45.000004	22
GTAATCG	30	2.1661108E-6	45.000004	43
CCGTAGA	20	7.034229E-4	45.000004	41
CTACGAC	20	7.034229E-4	45.000004	39
TACGACA	20	7.034229E-4	45.000004	20
GTCACGA	20	7.034229E-4	45.000004	36
GTCCGTT	35	1.2123746E-7	45.000004	13
GTCGTAC	20	7.034229E-4	45.000004	12
CGAATCG	20	7.034229E-4	45.000004	21
TTAACGA	30	2.1661108E-6	45.000004	2
ACTCGAT	20	7.034229E-4	45.000004	37
TCGTAGC	80	0.0	45.000004	2
TTACGAG	40	6.8175723E-9	45.000004	3
TACTAAT	20	7.034229E-4	45.000004	15
>>END_MODULE