FastQCFastQC Report
Thu 26 May 2016
SRR1545643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545643_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1374074
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT48080.3499083746581334TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT43200.3143935479457438TruSeq Adapter, Index 27 (96% over 26bp)
GTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC40290.29321564922995413TruSeq Adapter, Index 27 (96% over 25bp)
TGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC38310.27880594494910754TruSeq Adapter, Index 27 (100% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC36380.26476012208949445TruSeq Adapter, Index 27 (96% over 25bp)
TTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC35110.2555175339901636TruSeq Adapter, Index 27 (100% over 24bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC26890.1956954283393762TruSeq Adapter, Index 27 (96% over 25bp)
ATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC24950.1815768291955164TruSeq Adapter, Index 27 (100% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC19790.14402426652421924TruSeq Adapter, Index 27 (100% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC15530.11302156943512502TruSeq Adapter, Index 27 (100% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC14110.10268733707209364TruSeq Adapter, Index 27 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAC351.01976184E-746.00000410
AATCCCG206.311886E-446.00000433
GATCGAC405.6134013E-946.00000430
CATGCGA351.01976184E-746.00000433
CTATCGT206.311886E-446.00000418
ACGTTAC206.311886E-446.00000446
CGTTGTA206.311886E-446.00000431
GACAACG351.01976184E-746.00000422
CGATTCT351.01976184E-746.00000427
CCGCTTA206.311886E-446.00000434
TCGATAA351.01976184E-746.00000440
GTAATCG206.311886E-446.00000441
GTAATCA206.311886E-446.00000435
CCGTATG351.01976184E-746.00000416
TACGATT206.311886E-446.00000412
GTCCGTT206.311886E-446.00000411
CGAATTT206.311886E-446.00000423
CGTACAT351.01976184E-746.00000435
CGATGTA206.311886E-446.00000438
ATACGAT206.311886E-446.00000411
Produced by FastQC (version 0.11.5)