##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545643_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1374074
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52721978583395	33.0	31.0	34.0	31.0	34.0
2	32.94945541506498	34.0	31.0	34.0	31.0	34.0
3	33.03004860000262	34.0	33.0	34.0	31.0	34.0
4	36.39933147705291	37.0	37.0	37.0	35.0	37.0
5	36.37685816047753	37.0	37.0	37.0	35.0	37.0
6	36.39071112618389	37.0	37.0	37.0	35.0	37.0
7	36.4628244184811	37.0	37.0	37.0	35.0	37.0
8	36.50155741248288	37.0	37.0	37.0	35.0	37.0
9	38.30088044748682	39.0	39.0	39.0	37.0	39.0
10	38.059393453336575	39.0	39.0	39.0	35.0	39.0
11	37.89110775693303	39.0	38.0	39.0	35.0	39.0
12	37.54823539343587	39.0	37.0	39.0	35.0	39.0
13	37.479953772504246	39.0	37.0	39.0	35.0	39.0
14	38.81986705228394	41.0	38.0	41.0	35.0	41.0
15	38.8369898564415	41.0	38.0	41.0	35.0	41.0
16	38.90681579012484	41.0	38.0	41.0	35.0	41.0
17	38.85665109739359	41.0	38.0	41.0	35.0	41.0
18	38.84025532831565	41.0	38.0	41.0	35.0	41.0
19	38.8095772134543	40.0	38.0	41.0	35.0	41.0
20	38.721577586068875	40.0	38.0	41.0	35.0	41.0
21	38.65937933473743	40.0	38.0	41.0	35.0	41.0
22	38.72741642735399	40.0	38.0	41.0	35.0	41.0
23	38.73230772141821	40.0	38.0	41.0	35.0	41.0
24	38.69230114244211	40.0	38.0	41.0	35.0	41.0
25	38.604282593222784	40.0	37.0	41.0	35.0	41.0
26	38.515426388971775	40.0	37.0	41.0	35.0	41.0
27	38.43938754390229	40.0	37.0	41.0	35.0	41.0
28	38.38496252749124	40.0	36.0	41.0	35.0	41.0
29	38.35867136704428	40.0	36.0	41.0	35.0	41.0
30	38.263228181306104	40.0	36.0	41.0	34.0	41.0
31	38.25801303277698	40.0	36.0	41.0	34.0	41.0
32	38.18794766511847	40.0	36.0	41.0	34.0	41.0
33	38.110911784954816	40.0	36.0	41.0	34.0	41.0
34	38.03256520391187	40.0	36.0	41.0	34.0	41.0
35	37.9560758736429	40.0	35.0	41.0	34.0	41.0
36	37.8718227693705	40.0	35.0	41.0	34.0	41.0
37	37.77828413899106	40.0	35.0	41.0	34.0	41.0
38	37.7132134077204	40.0	35.0	41.0	34.0	41.0
39	37.61909911693257	40.0	35.0	41.0	33.0	41.0
40	37.4781758478801	40.0	35.0	41.0	33.0	41.0
41	37.34135352244493	39.0	35.0	41.0	33.0	41.0
42	37.25439241263571	39.0	35.0	41.0	33.0	41.0
43	37.17411726006023	39.0	35.0	41.0	33.0	41.0
44	37.0585164991114	39.0	35.0	41.0	33.0	41.0
45	36.97625600950167	39.0	35.0	41.0	33.0	41.0
46	36.95027342049991	39.0	35.0	41.0	33.0	41.0
47	36.93957530671565	39.0	35.0	41.0	33.0	41.0
48	36.86729754001604	39.0	35.0	41.0	33.0	41.0
49	36.78290979961778	38.0	35.0	41.0	33.0	41.0
50	36.64769801335299	38.0	35.0	40.0	32.0	41.0
51	36.52777579664559	38.0	35.0	40.0	32.0	41.0
52	36.17321337860989	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	3.0
10	2.0
11	5.0
12	6.0
13	18.0
14	18.0
15	35.0
16	74.0
17	116.0
18	227.0
19	440.0
20	694.0
21	1066.0
22	1566.0
23	2147.0
24	3046.0
25	4018.0
26	5216.0
27	6651.0
28	8097.0
29	10476.0
30	13495.0
31	17311.0
32	22710.0
33	31769.0
34	71629.0
35	150756.0
36	84707.0
37	115899.0
38	204417.0
39	616392.0
40	1063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.77710661871195	13.167049227334191	46.66509955067922	12.390744603274642
2	25.950276331551287	12.891954872881664	48.73936920427867	12.418399591288388
3	25.83281540877711	14.206731224082544	44.83594042242267	15.12451294471768
4	23.2594459978138	14.45977436440832	46.477773395028215	15.803006242749662
5	22.650017393531936	17.364785302683845	44.129792136376935	15.855405167407286
6	26.183524322561958	18.97808997186469	43.033999624474376	11.804386081098981
7	86.06872701179121	3.0432131020600055	6.458749674326128	4.429310211822653
8	86.98046830083386	3.2085608198685076	6.584507093504426	3.226463785793196
9	79.567767092602	4.705350657970386	8.850105598388442	6.876776651039172
10	43.17620448389243	23.4512842830881	14.74163691329579	18.630874319723684
11	34.66741965862101	22.845130611597337	24.376343632147904	18.11110609763375
12	30.316125623510814	23.828047106633267	26.267799259719638	19.58802801013628
13	22.70983949918272	25.962648299873226	32.46419042933641	18.863321771607644
14	18.750736859877996	34.124799683277615	27.59931415629726	19.52514930054713
15	16.807828399343848	24.79124122863834	37.37389689347153	21.02703347854628
16	20.685057718871036	21.99910630722945	36.132042379085846	21.18379359481367
17	22.12901197460981	20.954548299436563	27.31017397898512	29.606265746968504
18	22.085928414335765	23.734165699955025	29.798977347653764	24.380928538055446
19	26.043648304239802	25.170769550984883	26.59012542264827	22.195456722127048
20	28.7120635424293	22.034985015363073	24.33697166237044	24.915979779837187
21	26.400543202185617	24.9619743914811	27.391974522478414	21.245507883854874
22	29.267419367515867	21.41449441587571	23.103195315536134	26.214890901072284
23	26.74761330175813	25.568710273245838	22.433216842761016	25.250459582235017
24	26.779416537973937	20.17293100662701	28.61832768831955	24.429324767079503
25	28.417392367514417	22.575640031031806	24.702890819562846	24.30407678189093
26	23.788820689424295	27.05305536674153	23.424720939338055	25.733403004496118
27	22.067152133000114	26.06504453180833	23.76291233223247	28.104891002959082
28	22.55417102717903	26.719667208607394	27.63271847076649	23.093443293447077
29	21.93717368933551	23.20711984944042	25.765279016996175	29.090427444227895
30	22.00267234515754	25.175500009460915	25.242308638399386	27.579519006982157
31	23.117459467248487	26.890618700302895	24.985408355008538	25.00651347744008
32	25.29012265714947	23.652001275040497	25.488947465711455	25.568928602098577
33	25.235685996532936	24.50704983865498	25.430653662029844	24.826610502782238
34	21.269232952519296	23.82200667504079	28.023454340887028	26.885306031552886
35	22.003181779147265	22.999052452779107	29.814042038492833	25.18372372958079
36	23.97090695260954	24.290394840452553	25.791114597903753	25.947583609034158
37	23.550041700810873	26.809254814515082	24.82726548934046	24.813437995333583
38	22.609189898069538	24.22962664310656	28.888837136864538	24.272346321959372
39	22.83406861639184	26.189273649017448	26.085203562544667	24.891454172046046
40	25.083074128467608	22.843238428206924	29.267055486094634	22.806631957230834
41	22.093569924181665	23.171823351580773	27.752508234636565	26.982098489600997
42	21.883464791561444	25.127031004152617	24.2617937607436	28.727710443542342
43	22.426812529747306	22.655111733429205	26.55730331845301	28.360772418370484
44	26.404327568966444	23.429305845245597	24.986136117851004	25.180230467936955
45	21.638499818787054	23.776084839681126	25.803777671362678	28.781637670169147
46	19.875494332910744	25.558958251156778	26.719521656038904	27.84602575989357
47	23.023578060570244	23.479012047386092	28.675748176590197	24.821661715453462
48	21.949327328804706	20.685712705429257	31.620203860927433	25.744756104838608
49	20.856664197124754	21.987971535739707	27.327130853214605	29.82823341392094
50	19.8324107726367	24.020758707318528	30.072106742431632	26.07472377761314
51	22.036003883342527	23.021394772042843	27.834672659551085	27.10792868506354
52	21.709092814506352	21.954858326407457	28.7793816053575	27.556667253728694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	263.0
1	187.5
2	112.0
3	106.0
4	100.0
5	146.0
6	192.0
7	245.0
8	298.0
9	432.5
10	567.0
11	717.5
12	868.0
13	1003.0
14	1255.0
15	1372.0
16	1254.5
17	1137.0
18	1197.0
19	1257.0
20	1480.0
21	1703.0
22	1958.5
23	2214.0
24	3686.5
25	5159.0
26	5984.0
27	6809.0
28	7911.5
29	9014.0
30	11540.5
31	14067.0
32	17814.5
33	21562.0
34	24078.5
35	26595.0
36	28539.5
37	30484.0
38	33770.5
39	48906.5
40	60756.0
41	64620.0
42	68484.0
43	77484.0
44	86484.0
45	95553.0
46	104622.0
47	117532.5
48	130443.0
49	136837.5
50	143232.0
51	127759.5
52	112287.0
53	100181.5
54	88076.0
55	83215.0
56	78354.0
57	77294.5
58	76235.0
59	74144.5
60	72054.0
61	64204.5
62	56355.0
63	51220.0
64	39713.5
65	33342.0
66	26235.5
67	19129.0
68	15854.5
69	12580.0
70	11411.0
71	10242.0
72	7883.5
73	5525.0
74	4672.5
75	3820.0
76	3050.0
77	2280.0
78	1627.0
79	974.0
80	709.0
81	444.0
82	290.0
83	136.0
84	110.5
85	85.0
86	63.5
87	42.0
88	33.0
89	14.0
90	4.0
91	2.0
92	0.0
93	3.5
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1374074.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.56406697743609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.74959412011634	11.553369486545897
2	11.985853351287615	3.970689553849098
3	5.007682967158422	2.48842788209334
4	2.7253467050905247	1.8057130143941689
5	1.7164554828552372	1.4215741790900775
6	1.1225739488542492	1.115663404556802
7	0.7519088044421552	0.8718267458392628
8	0.536693807256007	0.711186573581094
9	0.41506359755482997	0.6187627106814404
>10	3.029181193198047	11.02400235500994
>50	1.1975751286209113	14.595787635374643
>100	1.7506402939567016	46.52124667354873
>500	0.005715299804479437	0.6433824295174858
>1k	0.005715299804479437	2.6583673559180325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT	4808	0.3499083746581334	TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	4320	0.3143935479457438	TruSeq Adapter, Index 27 (96% over 26bp)
GTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	4029	0.29321564922995413	TruSeq Adapter, Index 27 (96% over 25bp)
TGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3831	0.27880594494910754	TruSeq Adapter, Index 27 (100% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3638	0.26476012208949445	TruSeq Adapter, Index 27 (96% over 25bp)
TTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3511	0.2555175339901636	TruSeq Adapter, Index 27 (100% over 24bp)
GGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2689	0.1956954283393762	TruSeq Adapter, Index 27 (96% over 25bp)
ATCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	2495	0.1815768291955164	TruSeq Adapter, Index 27 (100% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1979	0.14402426652421924	TruSeq Adapter, Index 27 (100% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1553	0.11302156943512502	TruSeq Adapter, Index 27 (100% over 24bp)
TCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	1411	0.10268733707209364	TruSeq Adapter, Index 27 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3763261658396855	0.0
2	0.0	0.0	0.0	0.8916550345905679	0.0
3	0.0	0.0	0.0	3.1570352106218444	0.0
4	0.0	0.0	0.0	3.6420891451260995	0.0
5	0.0	0.0	0.0	4.434550104288415	0.0
6	0.0	0.0	0.0	6.7771459179054405	0.0
7	0.0	0.0	0.0	7.087391217649122	0.0
8	0.0	0.0	0.0	7.683356209345348	0.0
9	0.0	0.0	0.0	8.07234544864396	0.0
10	0.0	0.0	0.0	9.292148748902898	0.0
11	0.0	0.0	0.0	10.29224044702105	0.0
12	0.0	0.0	0.0	10.741561225960174	0.0
13	0.0	0.0	0.0	10.962000590943427	0.0
14	0.0	0.0	0.0	11.088340220395699	0.0
15	0.0	0.0	0.0	11.299027563289895	7.277628424669996E-5
16	0.0	0.0	0.0	11.79048581080786	7.277628424669996E-5
17	0.0	0.0	0.0	12.409884766031524	7.277628424669996E-5
18	7.277628424669996E-5	0.0	0.0	13.137865937351263	7.277628424669996E-5
19	7.277628424669996E-5	0.0	0.0	13.550143587608819	7.277628424669996E-5
20	7.277628424669996E-5	0.0	0.0	14.004704259013707	7.277628424669996E-5
21	7.277628424669996E-5	0.0	0.0	14.686836371258025	7.277628424669996E-5
22	7.277628424669996E-5	0.0	0.0	15.262496779649423	7.277628424669996E-5
23	7.277628424669996E-5	0.0	0.0	15.908095197201897	7.277628424669996E-5
24	1.4555256849339992E-4	0.0	0.0	16.384925411586277	7.277628424669996E-5
25	1.4555256849339992E-4	0.0	0.0	16.79894969266575	7.277628424669996E-5
26	1.4555256849339992E-4	0.0	0.0	17.175785292495164	7.277628424669996E-5
27	1.4555256849339992E-4	0.0	0.0	17.539375608591676	7.277628424669996E-5
28	1.4555256849339992E-4	0.0	0.0	17.880405276571715	7.277628424669996E-5
29	1.4555256849339992E-4	0.0	0.0	18.23599020140109	7.277628424669996E-5
30	1.4555256849339992E-4	0.0	0.0	18.635604778199717	7.277628424669996E-5
31	1.4555256849339992E-4	0.0	0.0	19.024302912361343	7.277628424669996E-5
32	1.4555256849339992E-4	0.0	0.0	19.422243634622298	7.277628424669996E-5
33	1.4555256849339992E-4	0.0	0.0	19.80664796801337	7.277628424669996E-5
34	1.4555256849339992E-4	0.0	0.0	20.175914834281123	7.277628424669996E-5
35	1.4555256849339992E-4	0.0	0.0	20.587173616559223	7.277628424669996E-5
36	2.183288527400999E-4	0.0	0.0	20.966993044042752	7.277628424669996E-5
37	2.183288527400999E-4	0.0	0.0	21.359402768700956	7.277628424669996E-5
38	2.183288527400999E-4	0.0	0.0	21.743661549523534	7.277628424669996E-5
39	2.183288527400999E-4	0.0	0.0	22.108998496441966	7.277628424669996E-5
40	2.183288527400999E-4	0.0	0.0	22.502499865363873	7.277628424669996E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAC	35	1.01976184E-7	46.000004	10
AATCCCG	20	6.311886E-4	46.000004	33
GATCGAC	40	5.6134013E-9	46.000004	30
CATGCGA	35	1.01976184E-7	46.000004	33
CTATCGT	20	6.311886E-4	46.000004	18
ACGTTAC	20	6.311886E-4	46.000004	46
CGTTGTA	20	6.311886E-4	46.000004	31
GACAACG	35	1.01976184E-7	46.000004	22
CGATTCT	35	1.01976184E-7	46.000004	27
CCGCTTA	20	6.311886E-4	46.000004	34
TCGATAA	35	1.01976184E-7	46.000004	40
GTAATCG	20	6.311886E-4	46.000004	41
GTAATCA	20	6.311886E-4	46.000004	35
CCGTATG	35	1.01976184E-7	46.000004	16
TACGATT	20	6.311886E-4	46.000004	12
GTCCGTT	20	6.311886E-4	46.000004	11
CGAATTT	20	6.311886E-4	46.000004	23
CGTACAT	35	1.01976184E-7	46.000004	35
CGATGTA	20	6.311886E-4	46.000004	38
ATACGAT	20	6.311886E-4	46.000004	11
>>END_MODULE