FastQCFastQC Report
Thu 26 May 2016
SRR1545642_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545642_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1077941
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC47610.44167537926472783Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29950.2778445202473976No Hit
ATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG21210.1967640158413123No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT18160.1684693318094404Illumina Single End Adapter 1 (95% over 22bp)
ATGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT16460.1526985243162659No Hit
GGGACTGTCTAGGGCACTCAGTGCCTCAAGTGGCAGGCAGCATAGAGAACA15880.14731789587741814No Hit
ATGCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC14750.13683494736724924No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC14070.13052662436997944Illumina Single End Adapter 1 (95% over 21bp)
ATACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC13960.12950616035571522No Hit
ATTGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC13940.1293206214440308No Hit
ATTTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT13550.12570261266618488No Hit
ATGTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC13390.12421830137270963No Hit
ATATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC13220.1226412206233922No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC12640.11726059218454443No Hit
ATGGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC11060.10260301816147636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT302.165376E-645.00000429
CGAACTA600.045.00000433
CGTATAG302.165376E-645.0000043
CACGGAT302.165376E-645.00000422
CGCATAT302.165376E-645.00000430
GCACGGA302.165376E-645.0000041
CGACATC302.165376E-645.00000413
AGGCGAA302.165376E-645.00000419
ATCGTCC302.165376E-645.0000041
GCCTATT302.165376E-645.00000420
CGTCGAT302.165376E-645.00000430
CGAGACA302.165376E-645.00000427
CGGTAGA302.165376E-645.00000424
CGTCCGA302.165376E-645.00000434
GCTCGAT600.045.00000424
ATCGCCT600.045.0000041
ATCGACT302.165376E-645.0000041
ACGGTCG302.165376E-645.00000421
TCTGCGA700.045.02
CTTGTAT351.211829E-745.026