FastQCFastQC Report
Thu 26 May 2016
SRR1545641_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545641_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences870650
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT37180.427037271004422Illumina Single End Adapter 1 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC26970.30976856371676337Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC24920.28622293688623446Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT24650.2831218055475794Illumina Single End Adapter 1 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC24050.2762304025727904Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC22470.2580830414058462Illumina Single End Adapter 1 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC19700.22626773100557054Illumina Single End Adapter 1 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC17780.20421524148624592Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC16530.1898581519554356Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC12890.14805030724171594Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC11200.1286395221960604Illumina Single End Adapter 1 (95% over 21bp)
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT10520.12082926549129959No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT9420.10819502670418653Illumina Single End Adapter 1 (95% over 22bp)
CTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC9200.10566817894676392Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT206.310607E-446.024
TGTCACG600.046.01
TGTCAAC253.416468E-546.045
CTATGCG453.092282E-1046.01
AACCGGC253.416468E-546.035
GTACCGG206.310607E-446.017
TACCCGG253.416468E-546.045
TCGCACC206.310607E-446.030
ACCGGAC253.416468E-546.030
TACTTCG206.310607E-446.029
CTCCGAC405.6079443E-946.022
TTTACGC301.8608789E-646.06
CATGCGT253.416468E-546.012
TCCGACA206.310607E-446.029
CCCTAAG253.416468E-546.045
ACCGCGA206.310607E-446.018
GACGTTA301.8608789E-646.045
ATAATCG301.8608789E-646.025
GAGTATA253.416468E-546.027
CGTGAGC301.8608789E-646.013
Produced by FastQC (version 0.11.5)