##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545641_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	870650
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53204272669844	33.0	31.0	34.0	31.0	34.0
2	32.94839717452478	34.0	31.0	34.0	31.0	34.0
3	33.03103887899845	34.0	33.0	34.0	31.0	34.0
4	36.415874346752425	37.0	37.0	37.0	35.0	37.0
5	36.38534772870844	37.0	37.0	37.0	35.0	37.0
6	36.39298110607018	37.0	37.0	37.0	35.0	37.0
7	36.46092459656578	37.0	37.0	37.0	35.0	37.0
8	36.50349164417389	37.0	37.0	37.0	35.0	37.0
9	38.32685005455694	39.0	39.0	39.0	37.0	39.0
10	38.07925802561305	39.0	39.0	39.0	35.0	39.0
11	37.89422041003848	39.0	38.0	39.0	35.0	39.0
12	37.43236202836961	39.0	37.0	39.0	35.0	39.0
13	37.31370470338253	39.0	37.0	39.0	35.0	39.0
14	38.58301958307012	40.0	37.0	41.0	35.0	41.0
15	38.64529604318613	40.0	37.0	41.0	35.0	41.0
16	38.73594440934934	41.0	37.0	41.0	35.0	41.0
17	38.687635674496065	40.0	37.0	41.0	35.0	41.0
18	38.693301556308505	40.0	37.0	41.0	35.0	41.0
19	38.68328260495032	40.0	37.0	41.0	35.0	41.0
20	38.58312410268191	40.0	37.0	41.0	35.0	41.0
21	38.519162694538565	40.0	36.0	41.0	35.0	41.0
22	38.60233963130994	40.0	37.0	41.0	35.0	41.0
23	38.59650376155746	40.0	36.0	41.0	35.0	41.0
24	38.55826451501751	40.0	36.0	41.0	35.0	41.0
25	38.49382989720324	40.0	36.0	41.0	35.0	41.0
26	38.36699707115373	40.0	36.0	41.0	35.0	41.0
27	38.25158444840062	40.0	35.0	41.0	35.0	41.0
28	38.18982828920921	40.0	36.0	41.0	34.0	41.0
29	38.16717624763108	40.0	36.0	41.0	35.0	41.0
30	38.109579050134954	40.0	36.0	41.0	34.0	41.0
31	38.06846723712169	40.0	35.0	41.0	34.0	41.0
32	37.990445069775454	40.0	35.0	41.0	34.0	41.0
33	37.90966519267214	40.0	35.0	41.0	34.0	41.0
34	37.84171710790788	40.0	35.0	41.0	34.0	41.0
35	37.76689140297479	40.0	35.0	41.0	34.0	41.0
36	37.67565152472291	40.0	35.0	41.0	34.0	41.0
37	37.56400275656119	40.0	35.0	41.0	33.0	41.0
38	37.48522598058921	40.0	35.0	41.0	33.0	41.0
39	37.448663642106474	39.0	35.0	41.0	33.0	41.0
40	37.2975581462126	39.0	35.0	41.0	33.0	41.0
41	37.19033365876069	39.0	35.0	41.0	33.0	41.0
42	37.15165795669902	39.0	35.0	41.0	33.0	41.0
43	37.05285935795096	39.0	35.0	41.0	33.0	41.0
44	36.912444725205305	38.0	35.0	41.0	33.0	41.0
45	36.83105495893872	38.0	35.0	41.0	33.0	41.0
46	36.825924309423996	38.0	35.0	41.0	33.0	41.0
47	36.80353873542755	38.0	35.0	41.0	33.0	41.0
48	36.73843909722621	38.0	35.0	40.0	33.0	41.0
49	36.69113191293861	38.0	35.0	40.0	33.0	41.0
50	36.58309998277149	38.0	35.0	40.0	33.0	41.0
51	36.479887440418075	37.0	35.0	40.0	32.0	41.0
52	36.115730775857116	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	2.0
12	2.0
13	6.0
14	10.0
15	15.0
16	39.0
17	62.0
18	151.0
19	229.0
20	413.0
21	707.0
22	916.0
23	1327.0
24	1894.0
25	2572.0
26	3336.0
27	4091.0
28	5295.0
29	6627.0
30	8699.0
31	11134.0
32	14770.0
33	21108.0
34	49339.0
35	115635.0
36	52126.0
37	73444.0
38	123987.0
39	372164.0
40	548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.52196634698214	14.117268707287659	46.04938838798599	12.311376557744214
2	25.298225463733992	13.572043875265608	48.94492620455981	12.18480445644059
3	25.10664446103486	13.670590937805088	45.849307988284615	15.373456612875438
4	22.64859587664389	14.725320163096537	48.017687934301954	14.608396025957617
5	23.442370642623327	17.143398610233735	45.412048469534255	14.002182277608682
6	24.30333658760696	19.493481881353013	44.092000229713435	12.111181301326596
7	85.62510767817149	2.8981795210474934	6.886234422557859	4.590478378223167
8	87.10388789984495	2.946419341871016	6.633090219950612	3.316602538333429
9	79.98529834032045	4.550967667834377	8.249239074254868	7.214494917590305
10	46.097168781944525	21.011198529834033	13.511054958938724	19.38057772928272
11	37.1771664848102	20.980416929879976	23.896054671796936	17.94636191351289
12	30.60058576925286	23.614655716993052	25.36874748750933	20.41601102624476
13	21.61075058864067	27.818066961465572	32.31769367713777	18.253488772755986
14	17.540687991730316	34.40073508298398	29.35932923677712	18.699247688508585
15	16.365933497961294	23.046344685005458	40.21903175788204	20.36869005915121
16	21.102050192385	20.7363464078562	36.07695399988514	22.08464939987366
17	20.451501751564923	21.681502325848502	27.480388215700913	30.38660770688566
18	22.01918107161316	21.837248033078733	32.032045023832765	24.111525871475333
19	26.250502498133578	22.878539022569345	27.288807213001782	23.582151266295295
20	27.062654338712456	22.148165163957962	24.927812553839086	25.861367943490492
21	24.3951071038879	26.001722850743697	28.50066042611842	21.102509619249986
22	28.878768736001838	20.382932291965773	25.19324642508471	25.545052546947684
23	25.131568368460343	24.956871303049446	22.708780795957043	27.202779532533167
24	25.053236087980245	20.478952506747834	30.763452592890367	23.704358812381553
25	27.756963188422446	22.198012978808936	25.815884683856886	24.22913914891173
26	21.800953310744845	27.266180439901223	24.707057945213347	26.225808304140585
27	22.084993970022396	25.306724860736228	24.241199104117612	28.367082065123757
28	21.84459886291851	26.120714408775054	29.831045770401428	22.203640957905012
29	22.214667202664675	22.88887612703153	27.880663871819905	27.01579279848389
30	20.881755010624246	26.661230115431	25.992649170160227	26.46436570378453
31	25.14581060127491	29.14649974157239	22.91368517774077	22.79400447941193
32	28.62918509159823	26.484006202262677	21.573651869293055	23.313156836846037
33	27.258255326480217	25.69206914374318	22.45644059036352	24.593234939413083
34	21.374834893470396	28.741400103371046	24.019181071613165	25.864583931545397
35	21.963016137368633	27.358180669614658	26.480675357491528	24.19812783552518
36	28.805145580887842	24.912421753862056	22.749554930224544	23.532877735025558
37	25.16970079825418	29.78360994659163	23.658301269166714	21.38838798598748
38	22.914604031470738	27.22425773847126	26.464250847068282	23.39688738298972
39	22.3558261069316	28.747946936197096	24.08464939987366	24.811577556997648
40	25.700683397461667	23.625222534887726	25.920404295641188	24.75368977200942
41	21.89387239418825	24.20984322058232	26.199620972836385	27.69666341239304
42	22.24843507724114	25.449032332165622	24.159421122150118	28.143111468443117
43	22.832481479354506	22.345029575604432	26.92827198070407	27.894216964336987
44	26.432780106816743	23.025440762648596	25.077470855108253	25.464308275426404
45	20.615402285648653	26.319186814448976	25.281456383162006	27.783954516740366
46	23.18049732958135	26.098087635674496	24.42979383219434	26.29162120254982
47	23.651180152759434	24.365933497961294	27.485097341067018	24.497789008212255
48	22.955952449319476	22.175271348992133	30.76506058691782	24.103715614770575
49	22.07477172227646	22.50376155745707	26.395681387469132	29.025785332797337
50	20.632630793085628	25.886751277780967	28.42324699936829	25.057370929765117
51	24.03721357606386	26.054097513352094	24.68236375122035	25.226325159363693
52	21.7094125078964	23.677137770631138	27.189341296732326	27.424108424740133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	318.0
1	225.0
2	132.0
3	119.5
4	107.0
5	120.0
6	133.0
7	220.5
8	308.0
9	328.5
10	349.0
11	487.0
12	625.0
13	841.5
14	1133.0
15	1208.0
16	935.0
17	662.0
18	735.5
19	809.0
20	1007.5
21	1206.0
22	1542.0
23	1878.0
24	1938.0
25	1998.0
26	2818.0
27	3638.0
28	4517.5
29	5397.0
30	6422.5
31	7448.0
32	8609.5
33	9771.0
34	11938.0
35	14105.0
36	14758.5
37	15412.0
38	19835.0
39	32454.0
40	40650.0
41	47687.5
42	54725.0
43	57345.0
44	59965.0
45	66625.0
46	73285.0
47	81063.0
48	88841.0
49	90338.0
50	91835.0
51	82040.0
52	72245.0
53	63860.5
54	55476.0
55	52905.5
56	50335.0
57	46742.5
58	43150.0
59	39825.5
60	36501.0
61	32593.5
62	28686.0
63	27069.0
64	23012.0
65	20572.0
66	16820.5
67	13069.0
68	11728.5
69	10388.0
70	8007.0
71	5626.0
72	4673.0
73	3720.0
74	3259.5
75	2799.0
76	1956.5
77	1114.0
78	906.0
79	698.0
80	556.5
81	415.0
82	282.5
83	150.0
84	110.0
85	70.0
86	49.5
87	29.0
88	29.0
89	15.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	870650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.120746015798854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.27974619441976	11.861209386336679
2	12.116253057177252	4.148785825101562
3	5.169557207967179	2.65520027915245
4	2.8360292751261054	1.9421974765121677
5	1.6672699327604166	1.4272452529284563
6	1.0993141697219873	1.1292647214827363
7	0.774756765491142	0.9285089664196975
8	0.5315638031579697	0.7280615092047756
9	0.42709844180561973	0.6581019551307717
>10	3.214376488451919	12.089330349612135
>50	1.1933473661743141	15.138127871236401
>100	1.6779349918086532	43.87987553643147
>500	0.004698217977064229	0.5544407273375055
>1k	0.008054087960681536	2.859650143113194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT	3718	0.427037271004422	Illumina Single End Adapter 1 (95% over 23bp)
GTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2697	0.30976856371676337	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2492	0.28622293688623446	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	2465	0.2831218055475794	Illumina Single End Adapter 1 (95% over 22bp)
TTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2405	0.2762304025727904	Illumina Single End Adapter 1 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2247	0.2580830414058462	Illumina Single End Adapter 1 (95% over 21bp)
ATCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1970	0.22626773100557054	Illumina Single End Adapter 1 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1778	0.20421524148624592	Illumina Single End Adapter 1 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1653	0.1898581519554356	Illumina Single End Adapter 1 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1289	0.14805030724171594	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1120	0.1286395221960604	Illumina Single End Adapter 1 (95% over 21bp)
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT	1052	0.12082926549129959	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	942	0.10819502670418653	Illumina Single End Adapter 1 (95% over 22bp)
CTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	920	0.10566817894676392	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.44472520530638027	0.0
2	1.148567162464825E-4	0.0	0.0	0.9645667030379601	0.0
3	1.148567162464825E-4	0.0	0.0	3.4463906276919545	0.0
4	1.148567162464825E-4	0.0	0.0	3.969562970194682	0.0
5	1.148567162464825E-4	0.0	0.0	4.777005685407454	0.0
6	1.148567162464825E-4	0.0	0.0	7.415264457589157	0.0
7	1.148567162464825E-4	0.0	0.0	7.7420318153104	0.0
8	1.148567162464825E-4	0.0	0.0	8.386492850169414	0.0
9	1.148567162464825E-4	0.0	0.0	8.790903348073279	0.0
10	1.148567162464825E-4	0.0	0.0	10.054671796933325	0.0
11	1.148567162464825E-4	0.0	0.0	11.17751105495894	0.0
12	1.148567162464825E-4	0.0	0.0	11.687934301958308	0.0
13	1.148567162464825E-4	0.0	0.0	11.913857462815137	0.0
14	1.148567162464825E-4	0.0	0.0	12.063860334233045	0.0
15	1.148567162464825E-4	0.0	0.0	12.280939527938896	0.0
16	1.148567162464825E-4	0.0	0.0	12.774249124217539	0.0
17	1.148567162464825E-4	0.0	0.0	13.466260839602596	0.0
18	1.148567162464825E-4	0.0	0.0	14.283581232412565	0.0
19	1.148567162464825E-4	0.0	0.0	14.75391948544191	0.0
20	2.29713432492965E-4	0.0	0.0	15.260897030953885	0.0
21	2.29713432492965E-4	0.0	0.0	16.017228507436972	0.0
22	2.29713432492965E-4	0.0	0.0	16.58427611554586	0.0
23	2.29713432492965E-4	0.0	0.0	17.23700683397462	0.0
24	3.4457014873944756E-4	0.0	0.0	17.72664101533337	0.0
25	4.5942686498593E-4	0.0	0.0	18.173893068397174	0.0
26	4.5942686498593E-4	0.0	0.0	18.577040142422327	0.0
27	4.5942686498593E-4	0.0	0.0	18.96560041348418	0.0
28	4.5942686498593E-4	0.0	0.0	19.34589100097628	0.0
29	4.5942686498593E-4	0.0	0.0	19.758800895882388	0.0
30	4.5942686498593E-4	0.0	0.0	20.20180325044507	0.0
31	4.5942686498593E-4	0.0	0.0	20.618962843852294	0.0
32	4.5942686498593E-4	0.0	0.0	21.019238499971287	0.0
33	4.5942686498593E-4	0.0	0.0	21.412163326250504	0.0
34	4.5942686498593E-4	0.0	0.0	21.790271636133923	0.0
35	4.5942686498593E-4	0.0	0.0	22.249239074254866	0.0
36	4.5942686498593E-4	0.0	0.0	22.660081548268536	0.0
37	4.5942686498593E-4	0.0	0.0	23.037500717854478	0.0
38	4.5942686498593E-4	0.0	0.0	23.435364382932292	0.0
39	4.5942686498593E-4	0.0	0.0	23.888244415092174	0.0
40	4.5942686498593E-4	0.0	0.0	24.307471429391832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	6.310607E-4	46.0	24
TGTCACG	60	0.0	46.0	1
TGTCAAC	25	3.416468E-5	46.0	45
CTATGCG	45	3.092282E-10	46.0	1
AACCGGC	25	3.416468E-5	46.0	35
GTACCGG	20	6.310607E-4	46.0	17
TACCCGG	25	3.416468E-5	46.0	45
TCGCACC	20	6.310607E-4	46.0	30
ACCGGAC	25	3.416468E-5	46.0	30
TACTTCG	20	6.310607E-4	46.0	29
CTCCGAC	40	5.6079443E-9	46.0	22
TTTACGC	30	1.8608789E-6	46.0	6
CATGCGT	25	3.416468E-5	46.0	12
TCCGACA	20	6.310607E-4	46.0	29
CCCTAAG	25	3.416468E-5	46.0	45
ACCGCGA	20	6.310607E-4	46.0	18
GACGTTA	30	1.8608789E-6	46.0	45
ATAATCG	30	1.8608789E-6	46.0	25
GAGTATA	25	3.416468E-5	46.0	27
CGTGAGC	30	1.8608789E-6	46.0	13
>>END_MODULE